Application of nanoscale secondary ion mass spectrometry to plant cell research

Kilburn, Matt R.; Jones, Davey L.; Clode, Peta L.; Cliff, John; Stockdale, E.; Herrmann, Anke M.; Murphy, Daniel V.

doi:10.4161/psb.5.6.11775

Cited by 30 publications

(17 citation statements)

References 21 publications

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“…NanoSIMS does enable simultaneous imaging and isotopic discrimination of stable isotopes (e.g. 13 C vs 12 C and 15 N vs 14 N) at a subcellular spatial scale (c. 100 nm resolution) (Kilburn et al, 2010). NanoSIMS techniques have been applied to investigate 15 N flow through microbes, protozoa, and animal cells (Foster et al, 2011;Pernice et al, 2012;Woebken et al, 2012), as well as rhizosphere bacteria and plant tissue (Clode et al, 2009;Jones et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Exploring the transfer of recent plant photosynthates to soil microbes: mycorrhizal pathway vs direct root exudation

et al. 2014

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Plants rapidly release photoassimilated carbon (C) to the soil via direct root exudation and associated mycorrhizal fungi, with both pathways promoting plant nutrient availability. This study aimed to explore these pathways from the root's vascular bundle to soil microbial communities.Using nanoscale secondary ion mass spectrometry (NanoSIMS) imaging and 13C-phospho- and neutral lipid fatty acids, we traced in-situ flows of recently photoassimilated C of 13CO2-exposed wheat (Triticum aestivum) through arbuscular mycorrhiza (AM) into root- and hyphae-associated soil microbial communities.Intraradical hyphae of AM fungi were significantly 13C-enriched compared to other root-cortex areas after 8 h of labelling. Immature fine root areas close to the root tip, where AM features were absent, showed signs of passive C loss and co-location of photoassimilates with nitrogen taken up from the soil solution. A significant and exclusively fresh proportion of 13C-photosynthates was delivered through the AM pathway and was utilised by different microbial groups compared to C directly released by roots.Our results indicate that a major release of recent photosynthates into soil leave plant roots via AM intraradical hyphae already upstream of passive root exudations. AM fungi may act as a rapid hub for translocating fresh plant C to soil microbes.

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Section: Introductionmentioning

confidence: 99%

Exploring the transfer of recent plant photosynthates to soil microbes: mycorrhizal pathway vs direct root exudation

et al. 2014

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“…Here, we use in vivo nanoscale secondary ion mass spectroscopy (NanoSIMS) analysis to provide quantitative high‐resolution spatial imaging of elements (Kilburn et al,2010) in the 24 hr after partial injury to a white matter tract of the CNS. The high‐energy ion beam of the NanoSIMS destroys bonds between atoms in the sample, so valency is unknown, so the term Ca ions is used when referring to NanoSIMS analyses.…”

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confidence: 99%

Early in vivo changes in calcium ions, oxidative stress markers, and ion channel immunoreactivity following partial injury to the optic nerve

Wells

Kilburn

Shaw

et al. 2011

J of Neuroscience Research

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CNS injury is often localized but can be followed by more widespread secondary degenerative events that usually result in greater functional loss. Using a partial transection model in rat optic nerve (ON). we recently demonstrated in vivo increases in the oxidative stress-associated enzyme MnSOD 5 min after injury. However, mechanisms by which early oxidative stress spreads remain unclear. In the present study, we assessed ion distributions, additional oxidative stress indicators, and ion channel immunoreactivity in ON in the first 24 hr after partial transection. Using nanoscale secondary ion mass spectroscopy (NanoSIMS), we demonstrate changes in the distribution pattern of Ca ions following partial ON transection. Regions of elevated Ca ions in normal ON in vivo rapidly decrease following partial ON transection, but there is an increasingly punctate distribution at 5 min and 24 hr after injury. We also show rapid decreases in catalase activity and later increases in immunoreactivity of the advanced glycation end product carboxymethyl lysine in astrocytes. Increased oxidative stress in astrocytes is accompanied by significantly increased immunoreactivity of the AMPA receptor subunit GluR1 and aquaporin 4 (AQP4). Taken together, the results indicate that Ca ion changes and oxidative stress are early events following partial ON injury that are associated with changes in GluR1 AMPA receptor subunits and altered ionic balance resulting from increased AQP4.

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“…Except for the rare gases, NanoSIMS is able to analyze all the elements or isotopes from hydrogen to uranium. Nowadays, NanoSIMS has been widely used in a variety of fields, such as materialogy (Hong et al, 2012;Lozano-Perez et al, 2008), cosmochemistry (Gyngard et al, 2010;Hoppe et al, 2013), phytoecology (Kilburn et al, 2010;Moore et al, 2010, 2012), geology (McLoughlin et al, 2011Wacey et al, 2008) and edaphology (Heister et al, 2012;Mueller et al, 2012).…”

Section: Principle and Featuresmentioning

confidence: 99%

A critical review of NanoSIMS in analysis of microbial metabolic activities at single-cell level

Gao

Huang

2015

Critical Reviews in Biotechnology

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Over 3.8 billion years of evolution has enabled many microbial species a versatile metabolism. However, limited by experimental methods, some unique metabolism remains unknown or unclear. A major obstacle is to attribute the incorporation of certain nutrients into a noncultivable species out of a complex microbial community. Such difficulty could be solved if we are able to directly observe substrate uptake at the single-cell level. Nanoscale secondary ion mass spectrometry (NanoSIMS) is a powerful tool for revealing element distribution in nanometer-scale resolution in the fields such as material sciences, geosciences and astronomy. In this review, we focus on another applicability of NanoSIMS in microbiology. In such fields, physiological properties and metabolic activities of microorganisms can be revealed with a single-cell scale resolution by NanoSIMS solely or in combination with other techniques. This review will highlight the features of NanoSIMS in analyzing the metabolic activities of carbon, nitrogen, metal irons by mixed-culture assemblies. Some values of NanoSIMS in environmental microbiology are expected to be discussed via this review.

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Application of nanoscale secondary ion mass spectrometry to plant cell research

Cited by 30 publications

References 21 publications

Exploring the transfer of recent plant photosynthates to soil microbes: mycorrhizal pathway vs direct root exudation

Exploring the transfer of recent plant photosynthates to soil microbes: mycorrhizal pathway vs direct root exudation

Early in vivo changes in calcium ions, oxidative stress markers, and ion channel immunoreactivity following partial injury to the optic nerve

A critical review of NanoSIMS in analysis of microbial metabolic activities at single-cell level

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