H ERE in focus on the nano technology and biotechnology as new technique knock for textile multifunctional finishing process with highly effective and recent research concentrate and restrict on using this promising technology concomitant also advanced highly effective tools namely sonication, plasma, microwave for finding out how to reduce conventional techniques utilities. Nanotechnology is revolutionizing the way in which the functional properties are imparted to the textile materials. Cotton, the king of natural fibers, requires nanotechnological interventions to achieve the functionalities like antibacterial, UV protecting, super hydrophobic and flame retardancy. Nano-finishing also helps to impart these properties without affecting the inherent comfort nature of cotton. In addition, the amount of material required to impart the functional property is relatively less due to its nano-size range. Enzymes have become an indispensable part of textile processing and are utilized for a wide variety of applications in the modern textile industry. Various established enzyme-based processes that are currently used in the textile industry demonstrate the potential of industrial biotechnology to successfully provide 'greener' alternatives