2024
DOI: 10.1007/s11686-024-00821-2
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Application of Nested-qPCR-High Resolution Melting (HRM) Technology on Strongyloides stercoralis Isolates from Iran

Zohreh Fakhrieh-Kashan,
Reza Fotouhi-Ardakani,
Farzaneh Zahabiun
et al.

Abstract: PurposeStrongyloides stercoralis is a parasite with special characteristics presenting it as a unique nematode. Iran is an endemic area for S. stercoralis. In this study, nested-qPCR-High Resolution Melting (HRM) technologies were applied on some human isolates of S. stercoralis from this country by focusing on evolutionary genetics analysis. MethodsTwelve human isolates of S. stercoralis were collected from four endemic provinces of Iran. Genomic DNA was extracted from a single lariform larva for every isolat… Show more

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