Application of new ZnO nanoformulation and Ag/Fe/ZnO nanocomposites as water-based nanofluids to considerin vitrocytotoxic effects against MCF-7 breast cancer cells

Abstract

Novel formulations of nanocomposites derived from ZnO nanoparticles have provided potential biomedical applications as a new strategy for treatment of breast cancer. In this research, two types of ZnO nanomaterials were synthesized by sol-gel hydrothermal process and co-precipitation containing fast quenching and also surface modification methods. The cytotoxic effects on growth of the breast cancer cell lines MCF-7 were evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. Cell viability of the breast cancer cell line MCF-7 was reduced with increasing ZnO nanofluid concentrations at 48 and 72 h of treatment. The IC value of MCF-7 cells after 72 h of treatment with the first product ZnO (a) and second one ZnO

ZnO Q-dots as a potent therapeutic nanomedicine for in vitro cytotoxicity evaluation of mouth KB44, breast MCF7, colon HT29 and HeLa cancer cell lines, mouse ear swelling tests in vivo and its side effects using the animal model

“…A mixture of hexamethylenetetramine and triethanolamine in water-ethanol solution was used for amine hydrolysis under vigorous stirring in hot (80 C) and cold (0 C) fast-quenching conditions in the presence of various surfactant templates to obtain polar surface area and very fine nanoparticles. The pH was then adjusted to 8 and stirring was continued under reflux for 9 h. The obtained product was placed in an oven (80 C) for 6 d under hydrothermal process and the solvent was then completely evaporated [9,10,[25][26][27][28][29]. The resulting soya fatty acidcapped ZnO is highly reactive with energetic surfaces.…”

“…Surface modification or functionalization of ZnO QDs are additional promising and significant strategies as these compounds are not very stable and dissolve in water, making them unfavourable for application as valuable drugs. Thus, water-soluble, hydrophilic ZnO QDs ought to be focused on in accordance with surface luminescent mechanisms [9,10]. Various functional clinical supplements such as polymers, liposomes, proteins, organosilanes, fatty acids (oleic acid), folic acid (FA) [11], amines, inorganic acids, nanometal oxides, nanometals (Ag, Au and Fe), inorganic/organic hybrid materials, PEG (polyethylene glycol), dendrimers, chitosan encapsulated nanocarriers and organosiloxanes can be positioned on the surface of ZnO QDs and create smart surface reactive morphologies.…”

A greener approach towards the development of graphene–Ag loaded ZnO nanocomposites for acetone sensing applications

“…6,7 Within the large family of metal oxide nanoparticles, ZnO based nanocomposites are being used in various applications such as electronics, communication, sensors, cosmetics, environmental protection, biology and medicinal industry. [8][9][10][11][12][13][14][15] ZnO is an interesting material from several points of view. It is one of the few oxides that shows quantum connement effects in an experimentally accessible size range.…”

Atividade antimicrobiana de um nanocompósito de nanocristais baseado em óxido de zinco dopado com nanocristais de prata e óxido de prata

“…A maior eficiência na atividade bactericida das NPs é atribuída à diferença na estrutura da parede celular dos microrganismos. Tem sido demonstrado que a atividade antimicrobiana do ZnO é mais efetiva para bactérias gram-positivas, pois elas possuem uma estrutura de parede celular mais simples (17,18 (14). Outro trabalho observou a viabilidade de células T humanas primárias em concentrações tóxicas de ZnO tanto para as bactérias gram-negativas como para as bactérias gram-positivas e ainda assim também encontrou efeitos mínimos sobre a viabilidade das células (21).…”

“…Neste estudo, nanopartículas de ZnO dopadas com Ag foram sintetizadas por mecanismos mecânicos e investigadas quanto à atividade antimicrobiana. Verificou-se que tanto o aumento no teor de Ag quanto a diminuição no tamanho das partículas contribuíram significativamente para a eficiência antimicrobiana(16).A citotoxicidade das nanopartículas foram avaliadas pelo método MTT, assim como foi realizado por outros pesquisadores(17)(18)(19)(20). Hu e colaboradores (2018), verificaram a atividade citotóxica das nanopartículas de ZnO e prata sobre uma linhagem de fibroblasto e puderam 33 observar diminuição da citotoxicidade quando em combinação, tal resultado corrobora com o presente trabalho.…”