Application of NGS for studying the antibiotic resistant microbiome in Wrocław tap water

Abstract

Abstract. The NGS method was used to investigate the biodiversity of ARB dwelling in tap water, able to form biofilm on bullion media supplemented with antibiotics: β-lactam, fluoroquinolone, 3 rd generation cephalosporin and tetracycline. Biofilms formed by cold-and hot-tap water microorganisms were also examined. The presence of 10 ARGs in plasmid DNA of cultivated biomass was tested using a standard PCR. The analyses revealed that the most predominant families were Sphingomonadaceae, Paenibacillaceae, Enterobacteriaceae, Bacillaceae and Sphingomonadaceae in FQ, C3G, T, C-W and H-W samples, respectively (explanations of abbreviations in the text). The ARGs did not correlate with resistance phenotypes.

Spatiotemporal Changes of Antibiotic Resistance and Bacterial Communities in Drinking Water Distribution System in Wrocław, Poland

“…The occurrence of ARB and ARGs in tap water has been reported worldwide, as well as in DWDS in Wrocław [7,9,18,25,26,[30][31][32]36,37,39,40,42,43,[45][46][47][48][49][50][51][52][53][54][55][56][57][58][59][60]. To the authors' best knowledge, however, this is the first study characterizing spatiotemporal changes in ARB, ARGs, and bacterial communities in bulk water samples collected from a full-scale DWDS in East-Central Europe.…”

“…Many studies to date have aimed to detect ARB in drinking water [7,30,32,36,37,[39][40][41][42][43][44][45][46][47][48][49][50][52][53][54][55][56][57][58][59][60], and several of them failed to isolate resistant bacteria from tap water samples [41,44]. Nevertheless, antibiotic resistant Staphylococci, Enterobacteriaceae, Pseudomonas, Sphingomonadaceae, and Acinetobacter were found in tap water in Europe [42,43,45,49,50,[58][59][60], and antibiotic resistant Escherichia coli in Asia [36,56,57], for example.…”