Male fertility has been seen to be declining, prompting for more effective and accessible means of its assessment. AI methods have been effective towards predicting semen quality through a questionnaire-based information source comprising a selection of factors from the medical literature which have been seen to influence semen quality. Prior work has seen the application of supervised learning towards the prediction of semen quality, but since supervised learning hinges on the provision of data class labels it can be said to depend on an external intelligence intervention, which can translate towards further costs and resources in practical settings. In contrast, unsupervised learning methods partition data into clusters and groups based on an objective function and do not rely on the provision of class labels, and can allow for a fully automated flow of a prediction platform. In this paper we apply three unsupervised learning models with different model architectures, namely: Gaussian mixture model (GMM), K-means and spectral clustering (SC), alongside low dimensional embedding methods which include sparse autoencoder (SAE), principal component analysis (PCA) and robust principal component analysis (RPCA). The best results were obtained with a combination of the sparse autoencoder and the SC algorithm, which was likely due to its nonspecific and arbitrary cluster shape assumption. Further work would now involve the exploration of similar unsupervised learning algorithms with a similar framework to the SC to investigate the extent to which various clusters can be learned with maximal accuracy.