Application of oligonucleotide array technology for the rapid detection of pathogenic bacteria of foodborne infections

Hong, Bo; Jiang, Lifang; Y, Hu; Fang, D.Z.; Guo, Huizhen

doi:10.1016/j.mimet.2004.05.005

Cited by 66 publications

(45 citation statements)

References 31 publications

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“…The oligonucleotide microarray technology with nitrocellulose membrane as the carrier and nano gold coloring, which showed high sensitivity and specificity for E . c o l i , S a l m o n e l l a , S h i g e l l a , V. c h o l e r a e , V. parahaemolyticus, proteus, Listeria monocytogenes, Bacillus cereus List Rand, Clostridium botulinum, and Campylobacter jejuni (Hong et al 2005). Rapid detection of E. coli using an integrated handheld Spreeta TM SPR sensor was conducted, and then the colloid gold antibody as the secondary antibody was introduced; it expanded the detection signal and extended the binding process of the colloid gold antibody with microorganisms, which increased the detection precision from 10 6 to 10 1 CFU/mL (Yin et al 2005).…”

Section: Detection Of Diseasesmentioning

confidence: 99%

Nanotechnology in agriculture, livestock, and aquaculture in China. A review

Huang

Wang

Liu

et al. 2014

Agron. Sustain. Dev.

258

120

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Nanoscience emerged in the late 1980s and is developed and applied in China since the middle of the 1990s. Although nanotechnologies have been less developed in agronomy than other disciplines, due to less investment, nanotechnologies have the potential to improve agricultural production. Here, we review more than 200 reports involving nanoscience in agriculture, livestock, and aquaculture. The major points are as follows: (1) nanotechnologies used for seeds and water improved plant germination, growth, yield, and quality. (2) Nanotechnologies could increase the storage period for vegetables and fruits. (3) For livestock and poultry breeding, nanotechnologies improved animals immunity, oxidation resistance, and production and decreased antibiotic use and manure odor. For instance, the average daily gain of pig increased by 9.9-15.3 %, the ratio of feedstuff to weight decreased by 7.5-10.3 %, and the diarrhea rate decreased by 55.6-66.7 %. (4) Nanotechnologies for water disinfection in fishpond increased water quality and increased yields and survivals of fish and prawn. (5) Nanotechnologies for pesticides increased pesticide performance threefold and reduced cost by 50 %. (6) Nano urea increased the agronomic efficiency of nitrogen fertilization by 44.5 % and the grain yield by 10.2 %, versus normal urea. (7) Nanotechnologies are widely used for rapid detection and diagnosis, notably for clinical examination, food safety testing, and animal epidemic surveillance. (8) Nanotechnologies may also have adverse effects that are so far not well known.

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Section: Detection Of Diseasesmentioning

confidence: 99%

Nanotechnology in agriculture, livestock, and aquaculture in China. A review

Huang

Wang

Liu

et al. 2014

Agron. Sustain. Dev.

258

120

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“…The selected variable regions of the alignment were checked for self-binding, GC content, secondary structure, and melting temperature (Tm) by the Vector NTI (Invitrogen Corporation, Carlsbad, CA, US) software and screened for homology with other bacterial sequences using National Center for Biotechnology BLAST (Basic Local Alignment Search Tool). To detect 23S rDNA gene, the primers were designed from the conservation region of the two ends of this gene fragment as follows: 23S-F 5'-ACCAGGATTTTGGCTTAGAAG-3' (corresponding to E. coli 1051 -1071 nucleotide sequence of 23S rDNA gene) and 23S-R 5'-digoxigenin-CACTTACCCC-GACAAGGAAT-3' (corresponding to E. coli 1938 -957 nucleotide sequence of 23S rDNA gene) (11). The forward primer was labeled with a digoxigenin dye (Metabion, Germany).…”

Section: Primers and Probesmentioning

confidence: 99%

“…The oligonucleotide probes were fixed on the membrane, and cross linked by UV crosslinker for 30 seconds to allow binding of probes onto the nylon membrane. After cross linking, any unbounded oligonucleotides were removed by two times washing in 0.5x SSC, 0.1% sodium dodecyl sulfate (SDS) for two minutes at 37˚C (5,11). The strips were dried and stored at room temperature.…”

Section: Preparation Of the Oligonucleotide Arraymentioning

confidence: 99%

“…Over the past ten years, several molecular methods were increasingly used to improve the sensitivity and speed of diagnosis in clinical microbiology. Most of them have several advantages over traditional microbiological methods including shorter time needed for data analysis, low detection limits, higher specificity and sensitivity (2,10,11). DNA hybridization using oligonucleotide microarray is a specific technique to detect microbial pathogens.…”

Section: Introductionmentioning

confidence: 99%

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Simultaneous Detection of Escherichia coli, Salmonella enterica, Listeria monocytogenes and Bacillus cereus by Oligonucleotide Microarray

Sarshar¹,

Shahrokhi²,

Ranjbar³

et al. 2015

Int J Enteric Pathog

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Background:Traditional laboratory methods to detect pathogenic bacteria are time consuming and laborious. Therefore, it is essential to use powerful and reliable molecular methods for quick and simultaneous detection of microbial pathogens. Objectives: The current study aimed to evaluate the capability and efficiency of 23S rDNA sequence for rapid and simultaneous detection of four important food-borne pathogens by an oligonucleotide microarray technique. Materials and Methods:The 23S rDNA sequences of Escherichia coli, Salmonella enterica, Listeria monocytogenes and Bacillus cereus were obtained from GenBank databases and used to design the oligonucleotide probes and primers by Vector NTI software. Oligonucleotide probes were placed on a nylon membrane and hybridization was performed between probes and 23S rDNA digoxigenin-labeled polymerase chain reaction (PCR) products. Hybridization signals were visualized by NBT/BCIP color development. Results: Positive hybridization color was produced for Escherichia coli, Salmonella enterica, Listeria monocytogenes and Bacillus cereus. The oligonucleotide microarray detected all bacterial strains in a single reaction in less than five hours. The sensitivity of the performed microarray assay was 10 3 cfu/mL for each species of pathogen. No cross reaction was found between the tested bacterial species. Conclusions: The obtained results indicated that amplification of 23S rDNA gene followed by oligonucleotide microarray hybridization is a rapid and reliable method to identify and discriminate foodborne pathogens tested under the study.

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“…Techniques like pathogenic-specific antibody coated magnetic beads [23,24] or hybridization of DNA fragments of bacteria [25], improves the analysis time, but still needs complex equipment and takes several hours to carry out. This could be improved by using IA.…”

Section: Introductionmentioning

confidence: 99%

Combined dielectrophoretic and impedance system for on‐chip controlled bacteria concentration: Application to Escherichia coli

et al. 2015

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Abbreviations: Impedance analysis (IA), escherichia coli (E. coli), digital lock-in (DLIA), analog-to-digital converter (ADC), finite element method (FEM). 2 AbstractThe present paper reports a bacteria autonomous controlled concentrator prototype with a userfriendly interface for bench-top applications. It is based on a micro-fluidic lab-on-a-chip and its associated custom instrumentation, which consists in a dielectrophoretic actuator, to preconcentrate the sample, and an impedance analyser, to measure concentrated bacteria levels.The system is composed by a single micro-fluidic chamber with interdigitated electrodes and custom electronics instrumentation. The prototype is supported by a real-time platform connected to a remote computer which automatically controls the system and displays impedance data which is used to monitor bacteria accumulation status on-chip. The system automates the whole concentrating operation. Performance has been studied for controlled volumes of Escherichia Coli (E. coli) samples injected into the micro-fluidic chip at constant flow rate of 10 µl/min. A media conductivity correcting protocol has been developed, as so as the preliminary results shown the distortion of impedance analyser measurement produced by bacterial media conductivity variations through time. With the correcting protocol, the measured impedance values were related to the quantity of bacteria concentrated with a correlation of 0.988 and a coefficient of variation of 3.1%. Feasibility of E. coli on-chip automated concentration, using the miniaturized system, has been demonstrated and impedance monitoring protocol adjusted and optimized, to handle electrical properties changes of the bacteria media over time.3

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Application of oligonucleotide array technology for the rapid detection of pathogenic bacteria of foodborne infections

Cited by 66 publications

References 31 publications

Nanotechnology in agriculture, livestock, and aquaculture in China. A review

Nanotechnology in agriculture, livestock, and aquaculture in China. A review

Simultaneous Detection of Escherichia coli, Salmonella enterica, Listeria monocytogenes and Bacillus cereus by Oligonucleotide Microarray

Combined dielectrophoretic and impedance system for on‐chip controlled bacteria concentration: Application to Escherichia coli

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