Application of phage peptide display technology for the study of food allergen epitopes

Abstract

Phage peptide display technology has been used to identify IgE-binding mimotopes (mimics of natural epitopes) that mimic conformational epitopes. This approach is effective in the characterization of those epitopes that are important for eliciting IgE-mediated allergic responses by food allergens and those that are responsible for cross-reactivity among allergenic food proteins. Application of this technology will increase our understanding of the mechanisms whereby food allergens elicit allergic reactions, will facilitate the discovery of diagnostic reagents and may lead to mimotope-based immunotherapy.

A comprehensive analysis of the allergenicity and IgE epitopes of myosinogen allergens in Scylla paramamosain

“…An alternative approach to analyse the allergic epitope is to screen a phage display library with allergen specific IgE antibodies to identify mimics of allergenic IgE epitopes, called mimotopes. This latter method can overcome the high cost and time‐consuming nature of peptide micro‐arrays, and also mitigate the limitations of crystal structure analysis, which is complicated and time‐consuming …”

“…This latter method can overcome the high cost and time-consuming nature of peptide micro-arrays, and also mitigate the limitations of crystal structure analysis, which is complicated and time-consuming. [21][22][23] In the present study, we screened 30 subjects with AC, confirming their anaphylaxis reactions by BAT. Moreover, IgE from AC was used as target protein.…”

Can alternative epitope mapping approaches increase the impact of B‐cell epitopes in food allergy diagnostics?

“…By using random libraries, peptides recognized by allergen-specific antibodies have been shown to consist of sequences mimicking a continuous or discontinuous epitope of the allergen; such peptides are called mimotopes and can be mapped on the 3D structure of the allergen by predictive webtools like EpiSearch. 10,56 A large drawback of these approaches is their failure to detect alterations in allergens caused by post-translational modifications or processing, and specific antibodies can only be detected against single epitopes and never in combination with others. Additionally, the assignment of mimotopes to surface patches of an allergen is solely based on in silico approaches and thus hampers the reliability of the outcome.…”

“…(aa [46][47][48][49][50][51][52][53][54][55][56][57][58][59][60] and Ara h3_140 (aa 418-432), which were recognized by few allergic patients. 72 In comparison, the sensitivity and specificity of intact Ara h 2 were defined as, respectively, 60%-100% and 60%-96%, showing no advantage using these two key marker epitopes.…”

Epitope Mapping of Major Ragweed Allergen Amb a 1

“…As such, they may be employed for the development of safer immunotherapies either based on carrier-bound peptide vaccines or based on hypoallergenic recombinant allergens. 22,23 In this study, we screened phage display libraries of random peptides using Amb a 1-specific IgG to define peptides mimicking Amb a 1 epitopes. Best mimotope candidates were tested for their IgE reactivity with sera of ragweed-allergic patients.…”