Application of phage therapy against red-fleshed kiwifruit canker

Bai, Jun; Liu, Yanxi; Liu, Mengjiao; Luo, Shiting; Yu-hong, Cheng; Li, Gang; Liu, Cheng; Wen, Shixiong; Xia, Mian; He, Xiaoqing; Jin, Yi

doi:10.1016/j.biocontrol.2022.104893

Cited by 9 publications

(8 citation statements)

References 46 publications

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“…Because the available treatments for kiwifruit canker have insufficient efficacy and environmental safety, novel antibacterial agents for controlling this disease are urgently required 33 . In this study, the newly designed Jelleine‐Ic peptide exhibited stronger activity against Psa than did the parent Jelleine‐I.…”

Section: Discussionmentioning

confidence: 85%

“…The control efficiency of the peptides against Psa in kiwifruit leaves was evaluated using 6-8-week-old potted A. chinensis 'Hongyang' as previously described with modifications. 33 Wounds were made on the leaf using a syringe needle and covered with filter papers (diameter = 1 cm) containing 50 μL of Psa (10 8 CFU/mL). After 2 h, 1 MIC of each peptide or AS was added.…”

Section: Kiwifruit Leaf Inoculation Assaymentioning

confidence: 99%

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Antibacterial mechanism of the novel antimicrobial peptide Jelleine‐Ic and its efficacy in controlling Pseudomonas syringae pv. actinidiae in kiwifruit

Zhang

Wang

Tang

et al. 2023

Pest Management Science

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BACKGROUNDBacterial canker caused by Pseudomonas syringae pv. actinidiae (Psa) poses a severe threat to kiwifruit production. Because of the insufficient efficacy and environmental safety of available treatments, novel antibacterial agents should be urgently developed. Antimicrobial peptides (AMPs) can be used as antimicrobials for disease control. In this study, we designed a novel AMP, Jelleine‐Ic, and evaluated its antibacterial activity and mechanism of action against Psa.RESULTSJelleine‐Ic with a half‐maximal effective concentration of 1.67 μg/mL exhibited stronger antibacterial activity than did parent Jelleine‐I. Jelleine‐Ic targeted the Psa membrane, increased membrane permeabilization and dissipated membrane potential, resulting in calcium leakage. Electron microscopy revealed that Jelleine‐Ic disrupted cell morphology and caused intracellular alterations. Moreover, this AMP penetrated the cell membrane, bound to DNA, and reduced the expression of genes related to DNA replication and repair. Jelleine‐Ic also reduced esterase activity and induced intracellular reactive oxygen species generation. This peptide inhibited the development of Psa canker. The control efficiency of Jelleine‐Ic against Psa in the leaf discs and leaves of kiwifruit was 81.83% and 70.53%, respectively, which was superior to that of the commercial agricultural streptomycin. Furthermore, Jelleine‐Ic upregulated the expression of kiwifruit defense genes (PR‐10 and WRKY70a).CONCLUSIONJelleine‐Ic effectively controls Psa in vitro and in vivo, and may be developed as a bactericide for plant disease control. © 2023 Society of Chemical Industry.

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Section: Discussionmentioning

confidence: 85%

Section: Kiwifruit Leaf Inoculation Assaymentioning

confidence: 99%

Antibacterial mechanism of the novel antimicrobial peptide Jelleine‐Ic and its efficacy in controlling Pseudomonas syringae pv. actinidiae in kiwifruit

Zhang

Wang

Tang

et al. 2023

Pest Management Science

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“…Another study demonstrated enhanced capacity of a combination of two RNA phages from the Siphoviridae family with two DNA phages from the Podoviridae family in controlling Psa in planta , utilizing artificially inoculated A. chinensis var. rufopulpa seedlings ( Bai et al., 2022 ). In particular, the authors observed stronger lytic capacity and prolonged growth of the phages within the mixture, when compared to their action alone ( Bai et al., 2022 ).…”

Section: State-of-the-art On Sustainable Disease Management Tools To ...mentioning

confidence: 99%

“…rufopulpa seedlings ( Bai et al., 2022 ). In particular, the authors observed stronger lytic capacity and prolonged growth of the phages within the mixture, when compared to their action alone ( Bai et al., 2022 ).…”

Section: State-of-the-art On Sustainable Disease Management Tools To ...mentioning

confidence: 99%

Scientific and technological advances in the development of sustainable disease management tools: a case study on kiwifruit bacterial canker

Santos,

Nunes da Silva,

Vasconcelos

et al. 2024

Front. Plant Sci.

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Plant disease outbreaks are increasing in a world facing climate change and globalized markets, representing a serious threat to food security. Kiwifruit Bacterial Canker (KBC), caused by the bacterium Pseudomonas syringae pv. actinidiae (Psa), was selected as a case study for being an example of a pandemic disease that severely impacted crop production, leading to huge economic losses, and for the effort that has been made to control this disease. This review provides an in-depth and critical analysis on the scientific progress made for developing alternative tools for sustainable KBC management. Their status in terms of technological maturity is discussed and a set of opportunities and threats are also presented. The gradual replacement of susceptible kiwifruit cultivars, with more tolerant ones, significantly reduced KBC incidence and was a major milestone for Psa containment – which highlights the importance of plant breeding. Nonetheless, this is a very laborious process. Moreover, the potential threat of Psa evolving to more virulent biovars, or resistant lineages to existing control methods, strengthens the need of keep on exploring effective and more environmentally friendly tools for KBC management. Currently, plant elicitors and beneficial fungi and bacteria are already being used in the field with some degree of success. Precision agriculture technologies, for improving early disease detection and preventing pathogen dispersal, are also being developed and optimized. These include hyperspectral technologies and forecast models for Psa risk assessment, with the latter being slightly more advanced in terms of technological maturity. Additionally, plant protection products based on innovative formulations with molecules with antibacterial activity against Psa (e.g., essential oils, phages and antimicrobial peptides) have been validated primarily in laboratory trials and with few compounds already reaching field application. The lessons learned with this pandemic disease, and the acquired scientific and technological knowledge, can be of importance for sustainably managing other plant diseases and handling future pandemic outbreaks.

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“…actinidiae ( Psa ) is an epidemic disease during the production of kiwifruit (Vanneste, 2017 ; Froud et al, 2018 ), which often results in sudden and severe economic losses and limitations in kiwifruit cultivation worldwide (Luti et al, 2021 ). People have explored various management strategies for controlling this disease (Bai et al, 2022 ), including but not limited to the application of antibiotics or copper and the breeding of resistant varieties (Wicaksono et al, 2018 ). However, available measures for this disease remain insufficient.…”

Section: Introductionmentioning

confidence: 99%

Response of Soil Microbial Community Structure Mediated by Sulfur-Induced Resistance Against Kiwifruit Bacterial Canker

et al. 2022

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Kiwifruit bacterial canker caused by Pseudomonas syringae pv. actinidiae (Psa) is a major threat to kiwifruit worldwide, and effective control measures are still lacking. Sulfur, as a mineral, has been proved to improve plants' resistance to pathogens. It is of great significance to study the effect of sulfur on rhizosphere microorganisms in kiwifruit planting areas infected by Psa for controlling kiwifruit canker. In this study, the sulfur powder and organic fertilizer were mixed as base fertilizer to treat the soil in the area where kiwifruit bacterial canker occurs. We investigated the incidence of kiwifruit bacterial canker in 2018 and 2019 after sulfur application and the changes in microbial characteristics and community composition structure in the kiwifruit rhizosphere by using the plate-counting method and high-throughput sequencing technology. Fertilization treatments of kiwifruit roots with sulfur and organic fertilizer reduced kiwifruit bacterial canker severity. The diversity of soil microbial communities increased significantly after sulfur application in the range of 1.0~2.0 kg/m3. In particular, the bacterial genera level showed a high diversity after 2 years of sulfur application, reaching more than 516 genera. Furthermore, sulfur treatment resulted in a significant increase in specific microbial taxa, including members of the Acidothermus, norank_f__HSB_OF53-F07, and norank_f __Acidobacteriaceae__Subgroup_1. Moreover, the proportion of the dominant bacteria Acidothermus in the population showed an increasing trend. Altogether, the sulfur application is the key factor leading to microbial differences in kiwifruit rhizosphere soil. Appropriate sulfur can improve microbial structure characteristics of kiwifruit rhizosphere soil, increase bacterial diversity index, and change bacterial community composition structure.

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Application of phage therapy against red-fleshed kiwifruit canker

Cited by 9 publications

References 46 publications

Antibacterial mechanism of the novel antimicrobial peptide Jelleine‐Ic and its efficacy in controlling Pseudomonas syringae pv. actinidiae in kiwifruit

Antibacterial mechanism of the novel antimicrobial peptide Jelleine‐Ic and its efficacy in controlling Pseudomonas syringae pv. actinidiae in kiwifruit

Scientific and technological advances in the development of sustainable disease management tools: a case study on kiwifruit bacterial canker

Response of Soil Microbial Community Structure Mediated by Sulfur-Induced Resistance Against Kiwifruit Bacterial Canker

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