Application of Pig Manure Compost with Different Biochar Modifies the Antibiotic Resistome and Bacterial Community in Agriculture Soil

Zhang, Yuan; Chen, Minglong; Bao, Canxin; Wu, Jinping; Zhou, Jie; He, Wencheng; Shi, Xincheng; Li, Gang

doi:10.1007/s11270-022-05582-0

Cited by 10 publications

(2 citation statements)

References 53 publications

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“…Moreover, some genera were environment related, including Gelidibacter , associated with decomposition of pig carcasses ( 63 ); and Sulfuricurvum and Desulfatiferula , sulphur-reducing genera ( 64 ). On the other hand, two beneficial bacteria were detected, Anaerofustis , positively correlated with crude fibre digestibility ( 65 ), and Arenimonas , considered a soil probiotic ( 66 ).…”

Section: Discussionmentioning

confidence: 99%

Swine farm environmental microbiome: exploring microbial ecology and functionality across farms with high and low sanitary status

Marin,

Migura-García,

Rodríguez

et al. 2024

Front. Vet. Sci.

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IntroductionStringent regulations in pig farming, such as antibiotic control and the ban on certain additives and disinfectants, complicate disease control efforts. Despite the evolution of microbial communities inside the house environment, they maintain stability over the years, exhibiting characteristics specific to each type of production and, in some cases, unique to a particular company or farm production type. In addition, some infectious diseases are recurrent in specific farms, while other farms never present these diseases, suggesting a connection between the presence of these microorganisms in animals or their environment. Therefore, the aim of this study was to characterise environmental microbiomes of farms with high and low sanitary status, establishing the relationships between both, health status, environmental microbial ecology and its functionality.MethodsFor this purpose, 6 pig farms were environmentally sampled. Farms were affiliated with a production company that handle the majority of the pigs slaughtered in Spain. This study investigated the relationship among high health and low health status farms using high throughput 16S rRNA gene sequencing. In addition, to identify ecologically relevant functions and potential pathogens based on the 16S rRNA gene sequences obtained, functional Annotation with PROkaryotic TAXa (FAPROTAX) was performed.Results and DiscussionThis study reveals notable differences in microbial communities between farms with persistent health issues and those with good health outcomes, suggesting a need for protocols tailored to address specific challenges. The variation in microbial populations among farms underscores the need for specific and eco-friendly cleaning and disinfection protocols. These measures are key to enhancing the sustainability of livestock farming, ensuring safer products and boosting competitive edge in the market.

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Section: Discussionmentioning

confidence: 99%

Swine farm environmental microbiome: exploring microbial ecology and functionality across farms with high and low sanitary status

Marin,

Migura-García,

Rodríguez

et al. 2024

Front. Vet. Sci.

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“…Meanwhile, three sulfonamide resistance genes:sul1, sul2, sul3, five tetracyclic resistance genes: tetA, tetC, tetG, tetX, tetM, three quinolone resistance genes: qnrA, qnrB, qnrS and type I integron intl1 abundance were selected for detection in agricultural soils. The primer sequences and annealing temperatures were referred to previous literature (Fan, et al 2019;Yang, et al 2018;Zhang, et al 2022a;Zhang, et al 2022b;Zou, et al 2022).…”

Section: Detection and Quantification Of Target Genesmentioning

confidence: 99%

Spread of Plasmids Carrying Antibiotic Resistance Genes in Soil-Lettuce-Snail Food Chain

Zhang,

Ji,

Tang

et al. 2023

Preprint

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Agricultural activities such as fertilization change the composition of antibiotic resistance genes(ARGs) and their host bacteria in agricultural fields, while complex microbial activities contribute to the transfer of ARGs and their host bacteria into crops and their transmission to humans through the consumption of agricultural products. The spread of plasmid-mediated ARGs in the farm food chain is unknown, as is the risk of their transfer to human pathogenic bacteria. Therefore, this study constructed a farmland food chain with soil-lettuce-snail as a typical structure, added genetically engineered Pseudomonas fluorescens containing multidrug-resistant plasmid RP4 to track its spread in the farmland food chain, and used different fertilization methods (no fertilization, chemical fertilizer application and manure application) to explore its influence on the spread and diffusion of ARGs and intl1 in the farmland food chain. It was found that exogenous Pseudomonas can enter plants from soil and pass into snails' intestines, and there is horizontal gene transfer phenomenon of RP4 plasmid in bacteria. The addition of exogenous drug-resistant bacteria had different effects on the total abundance of ARGs and intl1 at different interfaces of the food chain, but fertilization, especially manure, not only promoted the spread of Pseudomonas aeruginosa and the transfer of RP4 plasmid levels, but also significantly increased the total abundance of ARGs and intl1 at all interfaces of the food chain. The main ARGs host bacteria in the food chain include Proteobacteria, Bacteroides and Firmicutes, while Flavobacterium of Bacteroides is the unique potential host bacteria of RP4 plasmid, and fertilization obviously increases the abundance of Bacteroides. To sum up, this study provides reference for the risk assessment of ARGs spreading to human body through food chain.

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Effects of magnesium-modified biochar on antibiotic resistance genes and microbial communities in chicken manure composting

Liu,

Shi,

Liu

et al. 2023

Environ Sci Pollut Res

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Application of Pig Manure Compost with Different Biochar Modifies the Antibiotic Resistome and Bacterial Community in Agriculture Soil

Cited by 10 publications

References 53 publications

Swine farm environmental microbiome: exploring microbial ecology and functionality across farms with high and low sanitary status

Swine farm environmental microbiome: exploring microbial ecology and functionality across farms with high and low sanitary status

Spread of Plasmids Carrying Antibiotic Resistance Genes in Soil-Lettuce-Snail Food Chain

Effects of magnesium-modified biochar on antibiotic resistance genes and microbial communities in chicken manure composting

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