Application of Plant Biotechnology

Bhatia, Saurabh

doi:10.1016/b978-0-12-802221-4.00005-4

Cited by 17 publications

(21 citation statements)

References 112 publications

(132 reference statements)

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“…Despite the reality of crop intensification, including genetic engineering, the possible consequences of the presence of transgenes for the in vitro performance of populations are poorly documented. In this study, we present results that suggest detrimental consequences for the in vitro culture performance of wild cotton populations in the presence of transgenes, which calls for monitoring transgenes in the plants to be micropropagated for conservation or future genetic improvement, as has been suggested by other authors, as conservation strategies and protocols (Bhatia, 2015). Moreover, it is worth noting that in the present study, with a minimal investment of three primer sets for transgene detection, we were able to identify 23 out of 33 transformation events reported for cotton populations in Mexico (ISAAA, 2018).…”

Section: Discussionmentioning

confidence: 76%

In vitro performance in cotton plants with different genetic backgrounds: the case of Gossypium hirsutum in Mexico, and its implications for germplasm conservation

Hernández‐Terán

Wegier

Benítez

et al. 2019

PeerJ

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One of the best ex situ conservation strategies for wild germplasm is in vitro conservation of genetic banks. The success of in vitro conservation relies heavily on the micropropagation or performance of the species of interest. In the context of global change, crop production challenges and climate change, we face a reality of intensified crop production strategies, including genetic engineering, which can negatively impact biodiversity conservation. However, the possible consequences of transgene presence for the in vitro performance of populations and its implications for biodiversity conservation are poorly documented. In this study we analyzed experimental evidence of the potential effects of transgene presence on the in vitro performance of Gossypium hirsutum L. populations, representing the Mexican genetic diversity of the species, and reflect on the implications of such presence for ex situ genetic conservation of the natural variation of the species. We followed an experimental in vitro performance approach, in which we included individuals from different wild cotton populations as well as individuals from domesticated populations, in order to differentiate the effects of domestication traits dragged into the wild germplasm pool via gene flow from the effects of transgene presence. We evaluated the in vitro performance of five traits related to plant establishment (N = 300): propagation rate, leaf production rate, height increase rate, microbial growth and root development. Then we conducted statistical tests (PERMANOVA, Wilcoxon post-hoc tests, and NMDS multivariate analyses) to evaluate the differences in the in vitro performance of the studied populations. Although direct causality of the transgenes to observed phenotypes requires strict control of genotypes, the overall results suggest detrimental consequences for the in vitro culture performance of wild cotton populations in the presence of transgenes. This provides experimental, statistically sound evidence to support the implementation of transgene screening of plants to reduce time and economic costs in in vitro establishment, thus contributing to the overarching goal of germplasm conservation for future adaptation.

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Section: Discussionmentioning

confidence: 76%

In vitro performance in cotton plants with different genetic backgrounds: the case of Gossypium hirsutum in Mexico, and its implications for germplasm conservation

Hernández‐Terán

Wegier

Benítez

et al. 2019

PeerJ

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“…The callus (plural calli) is a group of plant cells, unorganized in a tissue form, which, given the right hormonal signals, can regenerate into different organs and even into a whole plant. 32 Calli are widely used in plant tissue culture as a critical step for plant genome editing; therefore, their growth is highly important. Two types of hydrogels, IdA 1 /CS 1 and UdA 1 / CS 1 , were used for callus growth, and their performance was compared with the commercial callus-inducing medium (CIM).…”

Section: ■ Results and Discussionmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Nucleoside-Based Cross-Linkers for Hydrogels with Tunable Properties

Itzhakov

Tworowski

Sadot

et al. 2023

ACS Appl. Mater. Interfaces

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Herein, we report bioderived cross-linkers to create biopolymer-based hydrogels with tunable properties. Nucleosides (inosine and uridine) and ribose (pentose sugar lucking the nitrogenous base) were partially oxidized to yield inosine dialdehyde (IdA), uridine dialdehyde (UdA), and ribose dialdehyde (RdA). The dialdehydes were further used as crosslinkers with polysaccharide chitosan to form hydrogels. Depending on the cross-linker type and concentration, the hydrogels showed tunable rheological, mechanical, and liquid holding properties allowing the preparation of injectable, soft, and moldable hydrogels. Computational modeling and molecular dynamics simulations shed light on hydrogel formation and revealed that, in addition to covalent bonding, noncovalent interactions (π−π stacking, cation−π, and H-bonding) also significantly contributed to the cross-linking process. To demonstrate various application possibilities, the prepared hydrogels were used as a growth platform for plant cells, as injectable inks for layer-by-layer 3D printing applications, and as moldable hydrogels for soft lithography to replicate the microstructure of the plant. These findings suggest that the obtained tunable biocompatible hydrogels have the potential to be good candidates for various biotechnological applications.

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“…Although sucrose is the most popular sugar used in tissue cultures (Romano et al, 1995;Cuenca and Vieitez, 2000;Ovono et al, 2009;Yaseen et al, 2013), it sometimes inhibits chlorophyll formation and photosynthesis, making autotrophic growth less feasible (George et al, 2008;Bhatia, 2015). In comparison, fructose and raffinose were less effective for S.…”

Section: Discussionmentioning

confidence: 99%

Effects of the Culture Medium, pH Level, and Type of Sugar on the Growth of Sphagnum cuspidatulum Müll. Hal.

Sitthichoptham,

Wongkantrakorn,

Kraichak

et al. 2023

HST

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Owing to habitat modifications and climate change, Sphagnum moss has gradually disappeared from many tropical areas. In Thailand, Sphagnum cuspidatulum Müll. Hal., which was one of the most abundant species in the past, experienced a significant decline in 2022. The in vitro conservation of this species in an axenic culture in the laboratory has not been attempted, and therefore our work focused on the gametophytic cultivation of S. cuspidatulum using the tissue culture technique. After collecting samples in the field, a gametophyte of S. cuspidatulum was prepared by growing the moss in a growth chamber for three months to induce shoot tip elongation. The explants were cultured for six weeks in different formulas of culture media, initial pHs of the treatments, sugar types, and sugar concentrations. The results showed that the highest dry weight was produced with the 1/6MS treatment, followed by the 1/8MS and 1/4MS treatments. Different initial pHs did not affect the dry weight or total chlorophyll content. It was also found that the pHs of all of the treatments gradually declined during cultivation to less than 3.0. When we supplemented the media with sugar, the 1/4MS treatment supplemented with 1.5% to 2% glucose had the highest dry weight. This study was the first to use MS media for Sphagnum cultivation rather than traditional media such as Knop and B5. The results demonstrated the potential of using micropropagation for the mass production and ex situ conservation of S. cuspidatulum in Thailand.

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Application of Plant Biotechnology

Cited by 17 publications

References 112 publications

In vitro performance in cotton plants with different genetic backgrounds: the case of Gossypium hirsutum in Mexico, and its implications for germplasm conservation

In vitro performance in cotton plants with different genetic backgrounds: the case of Gossypium hirsutum in Mexico, and its implications for germplasm conservation

Nucleoside-Based Cross-Linkers for Hydrogels with Tunable Properties

Effects of the Culture Medium, pH Level, and Type of Sugar on the Growth of Sphagnum cuspidatulum Müll. Hal.

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