Application of Polarimetry to the Determination of the Purity of a Sugar

Abstract

The purpose of the present research work was the application of polarimetry in a sugar sample in order to determine the purity, when submitting the sample to an analytical analysis it was necessary to perform a dilution of the sample in water, which was treated and filter to avoid turbidity and thus guarantee polarimetric measurement, the result of the SAC-i-ATAGO equipment reading was + 6.351 ° at a temperature of 20.6 ° C and a λ of 589 nm this determination is analytical for determination of the amounts of purity before the inversion of the sugar, after the measurement carried out, the sample was treated with HCl with the intention of determining the angle of rotation in the absolute configuration of the sugar after the inversion of the same, with this a measured from -0.056 ° at a temperature of 21.9 ° C and a λ of 589 nm, from these data an analytical calculation was carried out to determine the concentration of sucrose and glucose in relevance to Data tabulated at temperatures of 20 ° C, analytical calculations suggest that the inversion of the rotation angle is in relation to the treatment in acid medium of the sample, in addition that the concentrations expressed were analyzed in a solution expressed in grams per 100 ml , estimated concentration for the determination of the angle before and after the inversion. For this reason it is possible to determine the concentration or purity of a sugar thanks to the absolute configuration that it presents in the polarized light deviation since that estimates the concentration of the solution within the variations of the turn rotation.