Application of Polypyrrole-Based Electrochemical Biosensor for the Early Diagnosis of Colorectal Cancer

Zhang, Xindan; Tan, Xuecai; Wang, Ping; Qin, Jieling

doi:10.3390/nano13040674

Cited by 15 publications

(6 citation statements)

References 169 publications

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“…To circumvent the density/thickness limitations of poly(PD) films, we alternatively modified Pt electrodes with thiophene- and pyrrole-based polymers. These polymers have been widely used in the field of electrochemistry, ,, and they are known to grow linearly from the electrode surface, thereby preventing the dense cross-linking seen with poly(PD) films. By using monomers with either a free amine or carboxylic acid side chain, we were able to make use of carbodiimide coupling for downstream tethering of functional groups (Figure A).…”

Section: Results and Discussionmentioning

confidence: 99%

Survey of Conductive Polymers for the Fabrication of Conformation Switching Nucleic Acid-Based Electrochemical Biosensors

Shaver,

Mallires,

Harris

et al. 2023

ACS Appl. Polym. Mater.

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Electrochemical biosensors are a continuously evolving technology with great potential for applications in human health. With the continuous glucose monitor as an example, these sensors are capable of accurately determining molecular concentrations directly in the human body. A specific class of biosensors, termed conformation switching nucleic acid-based electrochemical sensors (NBEs), relies on the affinity of oligonucleotides for molecular recognition and their conformational dynamics upon target binding for signal generation. Currently, most NBEs are fabricated via the self-assembly of alkylthiol monolayers on Au electrodes. However, this architecture is limited in terms of stability and the breadth of supporting materials with which it is compatible. Here, to explore alternative material options for the fabrication of NBE sensors, we form conductive polymers of aromatic amines, thiophenes, and pyrroles on platinum electrodes. Altering many parameters throughout the study, we determine the extent to which the polymers passivate the electrode surface and then couple redox reporters or reporter-modified nucleic acids. We discuss the limitations and benefits of each polymer for the formation of NBE sensors and provide future directions to continue the quest for alternative sensor materials.

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Section: Results and Discussionmentioning

confidence: 99%

Survey of Conductive Polymers for the Fabrication of Conformation Switching Nucleic Acid-Based Electrochemical Biosensors

Shaver,

Mallires,

Harris

et al. 2023

ACS Appl. Polym. Mater.

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“…MNPs coated with conductive polymers (e.g., PANI and PPy) can be used for biosensing applications [53]. For example, DNA or aptamer probes immobilized on polypyrrole or polyaniline-modified transducers/nanoparticles [73][74][75] may offer a conductive coating with high stability and biocompatibility for biorecognition.…”

Section: Organic Coatings/ligandsmentioning

confidence: 99%

“…polyaniline-modified transducers/nanoparticles [73][74][75] may offer a conductive coating with high stability and biocompatibility for biorecognition.…”

Section: Chitosanmentioning

confidence: 99%

Synthesis and Modification of Magnetic Nanoparticles for Biosensing and Bioassay Applications: A Review

Carinelli,

Luis-Sunga,

González-Mora

et al. 2023

Chemosensors

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Biosensors are analytical devices that use biological interactions to detect and quantify single molecules, clinical biomarkers, contaminants, allergens, and microorganisms. By coupling bioreceptors with transducers, such as nucleic acids or proteins, biosensors convert biological interactions into electrical signals. Electrochemical and optical transductions are the most widely used methods due to their high detection capability and compatibility with miniaturization. Biosensors are valuable in analytical chemistry, especially for health diagnostics, as they offer simplicity and sensitivity. Despite their usefulness, challenges persist in immobilizing biorecognition elements on the transducer surface, leading to issues such as loss of sensitivity and selectivity. To address these problems, the introduction of nanomaterials, in particular magnetic nanoparticles (MNPs) and magnetic beads, has been implemented. MNPs combine their magnetic properties with other interesting characteristics, such as their small size, high surface-to-volume ratio, easy handling, and excellent biocompatibility, resulting in improved specificity and sensitivity and reduced matrix effects. They can be tailored to specific applications and have been extensively used in various fields, including biosensing and clinical diagnosis. In addition, MNPs simplify sample preparation by isolating the target analytes via magnetic separation, thus reducing the analysis time and interference phenomena and improving the analytical performance of detection. The synthesis and modification of MNPs play a crucial role in adjusting their properties for different applications. This review presents an overview of the synthesis and surface modifications of magnetic nanoparticles and their contributions to the development of biosensors and bioassays for their applications across different areas. The future challenges of MNP synthesis and integration in assays are focused on their stability, multiplex detection, simplification and portability of test platforms, and in vivo applications, among other areas of development.

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“…Compared with the conventional PPy, nanostructured PPy possesses enhanced electrochemical activity, improved electrical conductivity and biocompatibility, facilitating its utilization in fabrication electrochemical biosensors [165]. Based on the PPy film interacted with graphene via the π-π stacking, the detection of DA in high sensitivity and excellent selectivity was achieved [166].…”

Section: Conductive Polymers Modified Graphene Biosensorsmentioning

confidence: 99%

Recent advances on the development of graphene‐based field‐effect transistors, electrochemical biosensors and electrochemiluminescence biosensors

Zhang

Chen

et al. 2023

Electroanalysis

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Graphene, a honeycomb lattice of carbon material with single‐atom‐layer structure, demonstrates extraordinary mechanical, thermal, chemical and electronic properties. Thus, it has sparked tremendous interests in various fields, such as energy storage and conversion devices, field‐effect transistors (FET), chemical sensors and biosensors. In this review, we will first focus on the synthesis method of graphene and the fabrication strategy of graphene‐based materials. Subsequently, the construction of graphene‐based biosensors are introduced, in which three kinds of biosensors are discussed in details, including the FET, electrochemical biosensors and electrochemiluminescence (ECL) biosensors. The performances of the state‐of‐the‐art biosensors on the detection of biomolecules are also displayed. Finally, we also highlight some critical challenges remain to be solved and the development in this field for further research.

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Application of Polypyrrole-Based Electrochemical Biosensor for the Early Diagnosis of Colorectal Cancer

Cited by 15 publications

References 169 publications

Survey of Conductive Polymers for the Fabrication of Conformation Switching Nucleic Acid-Based Electrochemical Biosensors

Survey of Conductive Polymers for the Fabrication of Conformation Switching Nucleic Acid-Based Electrochemical Biosensors

Synthesis and Modification of Magnetic Nanoparticles for Biosensing and Bioassay Applications: A Review

Recent advances on the development of graphene‐based field‐effect transistors, electrochemical biosensors and electrochemiluminescence biosensors

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