Application of preserved boar semen for artificial insemination: Past, present and future challenges

Waberski, Dagmar; Riesenbeck, A; Schulze, Martin; Weitze, K. F.; Johnson, Lawrence A.

doi:10.1016/j.theriogenology.2019.05.030

Cited by 139 publications

(153 citation statements)

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“…Artificial insemination (AI) is one of the major breakthroughs of pig reproductive biotechnology and has become the main technique for the breeding of this species worldwide, being an essential tool to achieve productivity challenges in swine industry [1]. Although AI can be performed using both frozen-thawed and liquid-stored sperm at 17 • C, the latter is used in the vast majority of pig AI [2,3].…”

Section: Introductionmentioning

confidence: 99%

Exploring Seminal Plasma GSTM3 as a Quality and In Vivo Fertility Biomarker in Pigs—Relationship with Sperm Morphology

et al. 2020

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Glutathione S-transferases Mu 3 (GSTM3) is an essential antioxidant enzyme whose presence in sperm has recently been related to sperm cryotolerance, quality and fertility. However, its role in seminal plasma (SP) as a predictor of the same sperm parameters has never been investigated. Herein, cell biology and proteomic approaches were performed to explore the presence, origin and role of SP-GSTM3 as a sperm quality and in vivo fertility biomarker. GSTM3 in SP was quantified using a commercial Enzyme-Linked Immunosorbent Assay (ELISA) kit specific for Sus scrofa, whereas the presence of GSTM3 in testis, epididymis and accessory sex glands was assessed through immunoblotting analysis. Sperm quality and functionality parameters were evaluated in semen samples at 0 and 72 h of liquid-storage, whereas fertility parameters were recorded over a 12-months as farrowing rate and litter size. The presence and concentration of GSTM3 in SP was established for the first time in mammalian species, predominantly synthesized in the epididymis. The present study also evidenced a relationship between SP-GSTM3 and sperm morphology and suggested it is involved in epididymal maturation rather than in ejaculated sperm physiology. Finally, the data reported herein ruled out the role of this antioxidant enzyme as a quality and in vivo fertility biomarker of pig sperm.

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Section: Introductionmentioning

confidence: 99%

Exploring Seminal Plasma GSTM3 as a Quality and In Vivo Fertility Biomarker in Pigs—Relationship with Sperm Morphology

et al. 2020

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“…Antibiotics are a standard ingredient in diluents for boar semen, and are one of the key factors for preserving the vitality of spermatozoa and the overall fertilization ability of the insemination dose (Althouse, 2005;Althouse, 2008, Waberski et al, 2019. The long-term use of antibiotics in diluents for boar semen often results in the development of bacterial resistance, so antibiotics need to be changed periodically.…”

Section: ____________________________________________________________mentioning

confidence: 99%

Effects of Antibiotic Diluent Additives on the Motility Parameters and Morphological Integrity of Boar Sperm During Six Days of Storage

Jotanović

Peno

Mandić

et al. 2019

Contemporary Agriculture

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Summary The purpose of this paper is to examine the effect of antibiotic diluent additives on the motility and morphological integrity of diluted fresh boar semen during a six-day storage period. A total of 60 insemination doses, originating from two Landrace boars, were examined and allocated to control (C, n=30, diluted with BTS) and experimental groups (E, n=30, diluted with BTS upon antibiotic addition). The treatment applied exerted positive effects on the preservation of progressive motility, percentage of live sperm and HOS test results (70.24 vs. 66.53%, 71.54 vs. 69.77%, 67.35 vs. 64.17% and 64.10 vs. 54.26%;91.15 vs. 90.02%, 88.38 vs. 85.55%, 81.50 vs. 76.13% and 74.53 vs. 68.72%; and 93.35 vs. 92.40%, 91.04 vs. 88.02 %, 84.67 vs. 78.15% and 77.27 vs. 69.44% HOS+ sperm for the 1st, 3rd, 5th and 6th day of storage, respectively). The results obtained indicate that the treatment applied has a favourable effect on preserving the quality parameters of diluted fresh boar semen during storage, resulting most likely from a reduction of bacterial contamination.

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“…As reported for many species, antimicrobial resistance is also increasing within the swine production industry [4]. Artificial insemination (AI) is the most common breeding procedure in pigs, used to fecundate more than 90% of the sows in the world's primary pork-producing countries [5,6]. Liquid semen refrigerated at 16 ± 1 • C, and preserved up to seven days in long-term extenders, is routinely used in pig farms and breeding centers around the world.…”

Section: Introductionmentioning

confidence: 99%

“…Liquid semen refrigerated at 16 ± 1 • C, and preserved up to seven days in long-term extenders, is routinely used in pig farms and breeding centers around the world. To control bacterial growth, antimicrobial agents are essential components of semen extenders [5]. In the European Union, these regulations are outlined in Council Directive 90/429/EEC [7].…”

Section: Introductionmentioning

confidence: 99%

“…Seventy to 90% of the volume of the semen dose is retrograde eliminated from the uterus after AI [8], thus considerable amounts of antibiotic and potentially resistant bacteria enter the manure. The progressive ban of certain antibiotics for veterinary use [9], reinforces the need for alternative antimicrobial strategies in boar semen extenders [5]. During the last decades, physical approaches were developed such as bacteria removal by colloid centrifugation [10] and microfiltration of seminal plasma [11], or the use of antimicrobial peptides [12,13].…”

Section: Introductionmentioning

confidence: 99%

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Preliminary Assessment of the Mucosal Toxicity of Tea Tree (Melaleuca alternifolia) and Rosemary (Rosmarinus officinalis) Essential Oils on Novel Porcine Uterus Models

Bertocchi

Rigillo

Elmi

et al. 2020

IJMS

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Antimicrobial resistance, an ever-growing global crisis, is strongly linked to the swine production industry. In previous studies, Melaleuca alternifolia and Rosmarinus officinalis essential oils have been evaluated for toxicity on porcine spermatozoa and for antimicrobial capabilities in artificial insemination doses, with the future perspective of their use as antibiotic alternatives. The aim of the present research was to develop and validate in vitro and ex vivo models of porcine uterine mucosa for the evaluation of mucosal toxicity of essential oils. The in vitro model assessed the toxicity of a wider range of concentrations of both essential oils (from 0.2 to 500 mg/mL) on sections of uterine tissue, while the ex vivo model was achieved by filling the uterine horns. The damage induced by the oils was assessed by Evans Blue (EB) permeability assay and histologically. The expression of ZO-1, a protein involved in the composition of tight junctions, was assessed through immunohistochemical and immunofluorescence analysis. The results showed that low concentrations (0.2–0.4 mg/mL) of both essential oils, already identified as non-spermicidal but still antimicrobial, did not alter the structure and permeability of the swine uterine mucosa. Overall, these findings strengthen the hypothesis of a safe use of essential oils in inseminating doses of boar to replace antibiotics.

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Application of preserved boar semen for artificial insemination: Past, present and future challenges

Cited by 139 publications

References 40 publications

Exploring Seminal Plasma GSTM3 as a Quality and In Vivo Fertility Biomarker in Pigs—Relationship with Sperm Morphology

Exploring Seminal Plasma GSTM3 as a Quality and In Vivo Fertility Biomarker in Pigs—Relationship with Sperm Morphology

Effects of Antibiotic Diluent Additives on the Motility Parameters and Morphological Integrity of Boar Sperm During Six Days of Storage

Preliminary Assessment of the Mucosal Toxicity of Tea Tree (Melaleuca alternifolia) and Rosemary (Rosmarinus officinalis) Essential Oils on Novel Porcine Uterus Models

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