Application of principal component analysis to multispectral-multimodal optical image analysis for malaria diagnostics

Omucheni, Dickson L; Kaduki, Kenneth A.; Bulimo, Wallace; Angeyo, H. K.

doi:10.1186/1475-2875-13-485

Cited by 31 publications

(16 citation statements)

References 32 publications

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“…The DNA and RNA dye measurements typically done in malaria cytometry require less sensitivity, and could be implemented in similar apparatus. A recent report on spectral imaging of malaria‐infected cells in a microscope illuminated by multiple LEDs suggests that Hz may be detected efficiently by transmission measurements in the 630–700 nm range without a need to use polarized light; we have also found evidence for this. In addition to its substantially lower cost and complexity, a multiparameter imager for malaria cytometry would offer the considerable advantage of being able to analyze slides.…”

Section: Discussionmentioning

confidence: 99%

Light depolarization measurements in malaria: A new job for an old friend

et al. 2015

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The use of flow cytometry in malaria research has increased over the last decade. Most approaches use nucleic acid stains to detect parasite DNA and RNA and require complex multi-color, multi-parameter analysis to reliably detect infected red blood cells (iRBCs). We recently described a novel and simpler approach to parasite detection based on flow cytometric measurement of scattered light depolarization caused by hemozoin (Hz), a pigment formed by parasite digestion of hemoglobin in iRBCs. Depolarization measurement by flow cytometry was described in 1987; however, patent issues restricted its use to a single manufacturer's hematology analyzers until 2009. Although we recently demonstrated that depolarization measurement of Hz, easily implemented on a bench top flow cytometer (Cyflow), provided useful information for malaria work, doubts regarding its application and utility remain in both the flow cytometry and malaria communities, at least in part because instrument manufacturers do not offer the option of measuring depolarized scatter. Under such circumstances, providing other researchers with guidance as to how to do this seemed to offer the most expeditious way to resolve the issue. We accordingly examined how several commercially available flow cytometers (CyFlow SL, MoFLo, Attune and Accuri C6) could be modified to detect depolarization due to the presence of free Hz on solution, or of Hz in leukocytes or erythrocytes from rodent or human blood. All were readily adapted, with substantially equivalent results obtained with lasers emitting over a wide wavelength range. Other instruments now available may also be modifiable for Hz measurement. Cytometric detection of Hz using depolarization is useful to study different aspects of malaria. Adding additional parameters, such as DNA content and base composition and RNA content, can demonstrably provide improved accuracy and sensitivity of parasite detection and characterization, allowing malaria researchers and eventually clinicians to benefit from cytometric technology. V C 2015 International Society for Advancement of Cytometry Key terms Key terms: polarization; depolarized side scatter; hemozoin; flow cytometry; malaria; light depolarization MALARIA remains one of the most important parasitic diseases, killing around 700,000 people each year (1). Reliable detection of parasites and identification of the species are crucial for clinical diagnosis, as well as for many research applications. Both may require differentiation of asexual and sexual forms, determination of different maturation stages, and reliable quantification of parasite density (numbers per unit volume of blood or percentage of infected red blood cells). Antimalarial drug development and assessment of resistance to drugs may additionally require evaluation of parasite metabolic state and viability. Cytometry could potentially provide an alternative to microscopy of Giemsa stained smears (2), the accepted method for diagnosis for over a century.Malaria parasites growing inside infected ...

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Section: Discussionmentioning

confidence: 99%

Light depolarization measurements in malaria: A new job for an old friend

et al. 2015

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“…The Raman and UV‐visible absorption spectrum of single RBCs have been reported in Refs. . During it's intra‐erythrocytic stage, malarial parasite feeds on Hb , thereby reducing Hb concentration within the Erythrocyte.…”

Section: Optofluidic Single‐cell Absorption Measurementmentioning

confidence: 99%

Optofluidic single-cell absorption flow analyzer for point-of-care diagnosis of malaria

et al. 2015

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In this work, an optofluidic flow analyzer, which can be used to perform malaria diagnosis at the point-of-care is demonstrated. The presented technique is based on quantitative optical absorption measurements carried out on a single cell level for a given population of Human Red Blood Cells (RBCs). By measuring the optical absorption of each RBC, the decrease in the Hemoglobin (Hb) concentration in the cytoplasm of the cell due to the invasion of malarial parasite is detected. Cells are assessed on a single cell basis, as they pass through a microfluidic channel. The proposed technique has been implemented with inexpensive off-the-shelf components like laser diode, photo-detector and a micro-controller. The ability of the optofluidic flow analyzer to asses about 308,049 cells within 3 minutes has been demonstrated. The presented technique is capable of detecting very low parasitemia levels with high sensitivity.

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“…The features are listed based on the feature weight [Jeyachidra, 2014]. Principal component analysis (PCA) can be used to extract the features (Wimmer, 2017;Omucheni, 2014). In order to extract some features from the original data set, PCA equation is applied, standardized data set equation.…”

Section: Introductionmentioning

confidence: 99%

Feature Extraction Performance Verification based on Ultra-wideband Imaging and Artificial Neural Network

Vijayasarveswari¹,

Jusoh²,

Sabapathy³

et al. 2019

Proceedings of the Proceedings of the 2nd International Conference on Advance and Scientific Innovation, ICASI 2019, 18 July, B

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Breast cancer is a second leading case among the women. Therefore, an efficiency breast cancer system is very important for accurate early detection. The selection of number of features is very important to avoid the system complexity and large processing time. This paper proposed a modified feature selection method for early breast cancer detection. Ultra-wideband (UWB) signals are transmitted and received using a pair of antenna. 1632 features are extracted from the received UWB signal and four statistical features (mean, median, maximum and minimum numbers) are selected from the extracted 1632 features. These features are fed into feed forward backpropagation neural network for breast cancer detection. The proposed features selection method is able to use to detect the breast cancer in terms of existence, location and size with average accuracy of 86.28%. The proposed feature selection method is able to increase the system performance.

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Application of principal component analysis to multispectral-multimodal optical image analysis for malaria diagnostics

Cited by 31 publications

References 32 publications

Light depolarization measurements in malaria: A new job for an old friend

Light depolarization measurements in malaria: A new job for an old friend

Optofluidic single-cell absorption flow analyzer for point-of-care diagnosis of malaria

Feature Extraction Performance Verification based on Ultra-wideband Imaging and Artificial Neural Network

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