Application of principal component regression to luminescence data for the screening of ciprofloxacin and enrofloxacin in animal tissues

Abstract

A new screening method for the analysis of enrofloxacin and ciprofloxacin in edible animal tissues is described. The method is based on the application of principal component regression to luminescence measurements after reaction of quinolones with terbium(III) in a micellar medium. The method was used, first, to discriminate between quinolone-containing or quinolone-not-containing samples (concentration below the detection limit) and, then, to quantify the sum of both analytes. Standards in a pure-water matrix, using the first three principal components, were used for the determination. RRMSE range from 4 to 10% depending on the analyte. Calibration was successfully applied to the analysis of spiked chicken and trout muscle at concentrations between 10 and 50 micrograms kg-1.

Multiresidue determination of fluoroquinolones in milk by column liquid chromatography with fluorescence and ultraviolet absorbance detection

“…Limitations include the analysis of only one or two FQs, relatively low recoveries, tedious sample preparation, and/or the incomplete separation of all FQs in one run [21,22]. Other non-routine techniques such as terbium(III)-sensitised luminescence [23,24], capillary isotachophoresis [25,26] or immunoaffinity chromatography [27] have also been applied. The analysis of the antimicrobials in bovine, porcine and poultry tissue and fish has been studied [17,20,[22][23][24]28].…”

“…Other non-routine techniques such as terbium(III)-sensitised luminescence [23,24], capillary isotachophoresis [25,26] or immunoaffinity chromatography [27] have also been applied. The analysis of the antimicrobials in bovine, porcine and poultry tissue and fish has been studied [17,20,[22][23][24]28]. However, only a few methods have focused on the determination of FQ residues in milk [14,19,20].…”

1, 2‐Dihydroisoquinoline‐N‐Acetic Acid Derivatives as New Carriers for Specific Brain Delivery I: Synthesis and Estimation of Oxidation Kinetics Using Multivariate Calibration Method

“…A convenient method for resolving the mixtures, which can in principle be applied to the present case, is the least squares analysis. Multivariate calibration methods applied to spectral data are increasingly used for pharmaceutical analysis [14][15][16][17][18][19]. Classical least squares (CLS) analysis is one of the simplest multivariate methods that can be performed with easily accessible statistical software.…”

Chemometrics Assisted Spectrophotometric Method for Simultaneous Determination of Certain Fluoroquinolones and Their Decarboxylated Degradants

“…Multivariate calibration methods applied to spectral data are being increasingly used for pharmaceutical analysis. [28][29][30][31][32][33][34][35] Classical least squares (CLS) analysis is one of the simplest multivariate methods that can be performed with easily accessible statistical software. In the present work we discuss the possibility of simultaneous determination of either LF or NF as representative examples of the commercial fluoroquinolones in presence of their decarboxylated degradation products by a simple, rapid and precise procedure, and subsequent application of the method to the analysis of laboratory prepared binary mixtures, laboratory degraded drug samples and some used and expired commercial formulations.…”