Application of Probe Electrospray Ionization Mass Spectrometry (PESI-MS) to Clinical Diagnosis: Solvent Effect on Lipid Analysis

Mandal, Mridul Kanti; Yoshimura, Kenichi; Chen, Lee Chuin; Yu, Zhan; Nakazawa, Tadao; Katoh, Ryohei; Fujii, Hideki; Takeda, Sén; Nonami, Hiroshi; Hiraoka, Kenzo

doi:10.1007/s13361-012-0462-3

Cited by 50 publications

(38 citation statements)

References 21 publications

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“…MRM analysis revealed a4 0% decrease in signal intensity when compared with the results from an open emitter ( Figure S4). TherESI phenomenon occurs with all the types of emitters tested, including capillaries of borosilicate,q uartz, or fused silica, steel needles, [12] thetashaped capillaries, [13] and those made of porous materials like wooden tips [14] and paper. by sputter-coating al ayer of Pd/Au; Figure S3).…”

Section: Methodsmentioning

confidence: 99%

On‐Demand Ambient Ionization of Picoliter Samples Using Charge Pulses

Hollerbach

Luo

et al. 2015

Angew Chem Int Ed

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Relay electrospray ionization (rESI) from a capillary containing a sample solution (or from an array of such capillaries) is triggered by charge deposition onto the capillary. Suitable sources of primary ions, besides electrosprays, are plasma ion and piezoelectric discharge plasma sources. With no requirement for physical contact, high-throughput sample screening is enabled by rapidly addressing individual secondary (sample) capillaries. Sub-pL sample volumes can be loaded and sprayed. Polar analytes, including neurotransmitters, phosphopeptides, oligonucleotides, illicit drugs, and pharmaceutical compounds are successfully ionized by rESI with concentration sensitivities (0.1 ppb for acetylcholine) which are similar to nanoESI but absolute sensitivities are orders of magnitude better. Nonpolar analytes (steroids, alkynes) are ionized by rESI using an open-tube secondary capillary and injecting electrolytically generated metal cations from the primary electrospray.

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Section: Methodsmentioning

confidence: 99%

On‐Demand Ambient Ionization of Picoliter Samples Using Charge Pulses

Hollerbach

Luo

et al. 2015

Angew Chem Int Ed

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“…Later, Hiraoka et al reported a technique called probe electrospray ionization (PESI), 36) which uses a stainless steel needle with micrometer-sized tip as the ESI emitter. PESI has been demonstrated to be useful in applications, such as clinical diagnosis, 37) detection of illicit drugs in body uids, 38) reaction monitoring, 39) and analysis of biological samples. 40) Solvent droplets over a solid metal substrate with high voltage undergo a process similar to in traditional electrospray, in which a metal-made hollow capillary is used to direct sample solution and as the ESI emitter.…”

Section: Esi Emitter Solid Metal Substratesmentioning

confidence: 99%

Electrospray Modifications for Advancing Mass Spectrometric Analysis

Meher

Chen

2017

Mass Spectrometry

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Generation of analyte ions in gas phase is a primary requirement for mass spectrometric analysis. One of the ionization techniques that can be used to generate gas phase ions is electrospray ionization (ESI). ESI is a so ionization method that can be used to analyze analytes ranging from small organics to large biomolecules. Numerous ionization techniques derived from ESI have been reported in the past two decades.ese ion sources are aimed to achieve simplicity and ease of operation. Many of these ionization methods allow the exibility for elimination or minimization of sample preparation steps prior to mass spectrometric analysis. Such ion sources have opened up new possibilities for taking scienti c challenges, which might be limited by the conventional ESI technique. us, the number of ESI variants continues to increase. is review provides an overview of ionization techniques based on the use of electrospray reported in recent years. Also, a brief discussion on the instrumentation, underlying processes, and selected applications is also presented.

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“…A new ionization method, probe electrospray ionization (PESI)–mass spectrometry (MS), which uses a solid needle as a sample probe and an ESI emitter, was developed by Hiraoka et al and can be applied not only to liquid and surface analyses, but also to the inside of samples. As PESI usually requires no pretreatment steps, PESI–MS has been used in several fields, such as food science, plant science, studies of chemical reaction mechanisms, medical and clinical diagnosis, and metabolomics . In this study, we used PESI–MS/MS for the direct detection of MT‐45, an opioid analgesic psychoactive substance, in human tissues in an actual forensic case.…”

Section: Introductionmentioning

confidence: 99%

Direct detection of the psychoactive substance MT‐45 in human tissue samples by probe electrospray ionization‐tandem mass spectrometry

Usui

Murata

Fujita

et al. 2018

Drug Testing and Analysis

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Analyses of drugs and poisons in tissue samples are essential in forensic toxicology and pharmacology. However, current procedures for tissue analysis are laborious and time-consuming. Therefore, we assessed the utility of a newly devised probe electrospray ionization (PESI) technique with tandem mass spectrometry (MS/MS) for easy, ultra-rapid drug detection in human tissue samples. Using this system, typical pretreatment procedures, such as solid-phase extraction, liquid-liquid extraction, deproteinization, or homogenization, can be avoided. Briefly, a tissue sample of 1-2 mm was supplemented with a solution of ethanol and 10 mmol/L ammonium formate, and measurements were obtained. We demonstrated the successful application of this method in a forensic case by detecting an opioid analgesic, MT-45, in all tissue samples (liver, kidney, lung, brain, and heart). We also detected oxidized metabolites of MT-45 in the liver. Since the analysis required only 0.5 minutes per sample, PESI-MS/MS is an ultra-rapid detection method. Furthermore, for a quantitative approach, the total analysis time for the combination of PESI-MS/MS with the quick, easy, cheap, effective, rugged, and safe (QuEChERS) extraction method (from instrument start-up to extraction and PESI-analysis) was within 8 minutes. MT-45 concentrations obtained by QuEChERS-PESI-MS/MS and liquid chromatography (LC) -MS/MS were similar for all tissue samples. PESI-MS/MS cannot be used to separate isobars/isomers (ie, compounds with the same m/z value), similar to other direct introduction techniques. Further studies are needed to validate the quantitation method. However, our results indicate that PESI-MS/MS is a potentially easy and rapid technique for the analysis of drugs and poisons in human tissue samples.

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Application of Probe Electrospray Ionization Mass Spectrometry (PESI-MS) to Clinical Diagnosis: Solvent Effect on Lipid Analysis

Cited by 50 publications

References 21 publications

On‐Demand Ambient Ionization of Picoliter Samples Using Charge Pulses

On‐Demand Ambient Ionization of Picoliter Samples Using Charge Pulses

Electrospray Modifications for Advancing Mass Spectrometric Analysis

Direct detection of the psychoactive substance MT‐45 in human tissue samples by probe electrospray ionization‐tandem mass spectrometry

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