Application of protein typing in molecular epidemiological investigation of nosocomial infection outbreak of aminoglycoside-resistant Pseudomonas aeruginosa

Abstract

Pseudomonas aeruginosan has emerged as an important pathogen elated to serious infections and nosocomial outbreaks worldwide. This study was conducted to understand the prevalence of aminoglycoside (AMG)-resistant P. aeruginosa in our hospital and to provide a scientific basis for control measures against nosocomial infections. Eighty-two strains of P. aeruginosa were isolated from clinical departments and divided into AMG-resistant strains and AMG-sensitive strains based on susceptibility test results. AMG-resistant strains were typed by drug resistance gene typing (DRGT) and protein typing. Five kinds of aminoglycoside-modifying enzyme (AME) genes were detected in the AMG-resistant group. AMG-resistant P. aeruginosa strains were classified into three types and six subtypes by DRGT. Four protein peaks, namely, 9900.02, 7600.04, 9101.25 and 10,372.87 Da, were significantly and differentially expressed between the two groups. AMG-resistant P. aeruginosa strains were also categorised into three types and six subtypes at the distance level of 10 by protein typing. AMG-resistant P. aeruginosa was cloned spread in our hospital; the timely implementation of nosocomial infection prevention and control strategies were needed in preventing outbreaks and epidemic of AMG-resistant P. aeruginosa. SELDI-TOF MS technology can be used for bacterial typing, which provides a new method of clinical epidemiological survey and nosocomial infection control.

Investigation of Cephalosporin and Heavy Metal Resistance of Aeromonas hydrophila and Pseudomonas aeruginosa Strains Isolated from Hospital Sewage in Türkiye

“…Aeromonas hydrophila and P. aeruginosa, which are opportunistic Gram-negative pathogens, have recently attracted much interest. There are some studies regarding antibacterial and heavy metal resistance of A. hydrophila and P. aeruginosa isolated from different sources, such as from aquatic environments (Piotrowska & Popowska, 2014), domestic water (Hassani et al, 1992), aquaculture (Matyar, 2007), fish (Filik et al, 2021), soil (Malik & Jaiswal, 2000), and nosocomial infections (Micek et al, 2015;Song et al, 2018). The present study is the first to determine the prevalence and resistance of A. hydrophila and P. aeruginosa from hospital sewage in Turkey to cephalosporins and heavy metals.…”

<em>Pseudomonas aeruginosa</em> bacteremia among liver transplant recipients

“…The outbreak of P. aeruginosa infection is generally considered to be the product of insufficient hand hygiene practices and contact precautions. 115 The measures of terminating the outbreak of P. aeruginosa infection consist of environmental investigation, intensification of hand hygiene and isolation measures, replacing or installing contaminated reservoirs, water network hyperchlorination, excreta management, use of gloves, and the decontamination of medical utilities. 101 , 104 , 105 , 110 – 113…”

“…For environmental investigation, the use of a molecular tool such as gene-based methods, for example pulsed-field gel electrophoresis or protein-based typing method involving surface-enhanced lasers desorption/ionization-time-of-flight MS, has been proposed to provide knowledge of the clonal origin of the outbreak and to find the accurate sources and transmission pathways, which are crucial for designing optimal infection control and prevention strategies and thus reduce the apparent scale of an outbreak. 101 , 114 , 115 Enhancing general infection control measures including hand hygiene and isolation measures led to a reduction in clinical cases in most studies but often did not completely terminate outbreaks. 116 Replacing reservoirs; point-of-use filtration; and decontaminating using bleach, hydrogen peroxide vapor, acetic acid, hot water, UV light, ozonation, and aldehyde-based disinfectants, were effective to end the outbreak.…”