Application of proteomics for comparison of proteome of Neospora caninum and Toxoplasma gondii tachyzoites

Lee, Eung-goo; Kim, Jae Hoon; Shin, Yoo In; Shin, Gee-Wook; Kim, Youngrim; Palaksha, K.J.; Kim, Dae-Yong; Yamane, Ichirō; Kim, Yonghwan; Kim, Gon-sup; Suh, Myung-deuk; Jung, Taehun

doi:10.1016/j.jchromb.2004.08.048

Cited by 30 publications

(18 citation statements)

References 46 publications

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“…These genotypes have highly different phenotypes in the laboratory mice, with type I strains being acutely virulent, type II strains intermediate virulence, and type III strains essentially avirulent [38]. PDI is highly expressed in T. gondii and acts as chaperones to aid protein folding by forming a bridge between two cysteines [39], as well as modulates the tachyzoite-host cell interaction [22], [23]. In addition, PDI is a conserved protein in evolution of Toxoplasma as the alignment of the amino acid sequence of PDI revealed a 100% homology among three strains of T. gondii (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…They functionality modulate cellular interactions with a number of pathogens [21]. For instance, PDIs from T. gondii and N. caninum have been identified at the surface of tachyzoites, and they modulate the tachyzoite-host cell interaction [22], [23]. Interestingly, 81% of individuals display an anti- T. gondii IgA antibody in their tears that can recognise T. gondii PDI (TgPDI) [24].…”

Section: Introductionmentioning

confidence: 99%

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Toxoplasma gondii Protein Disulfide Isomerase (TgPDI) Is a Novel Vaccine Candidate against Toxoplasmosis

Wang

Yin

et al. 2013

PLoS ONE

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Toxoplasma gondii is a ubiquitous protozoan parasite that can infect all warm-blooded animals, including both mammals and birds. Protein disulfide isomerase (PDI) localises to the surface of T. gondii tachyzoites and modulates the interactions between parasite and host cells. In this study, the protective efficacy of recombinant T. gondii PDI (rTgPDI) as a vaccine candidate against T. gondii infection in BALB/c mice was evaluated. rTgPDI was expressed and purified from Escherichia coli. Five groups of animals (10 animals/group) were immunised with 10, 20, 30, 40 μg of rTgPDI per mouse or with PBS as a control group. All immunisations were performed via the nasal route at 1, 14 and 21 days. Two weeks after the last immunisation, the immune responses were evaluated by lymphoproliferative assays and by cytokine and antibody measurements. The immunised mice were challenged with tachyzoites of the virulent T. gondii RH strain on the 14th day after the last immunisation. Following the challenge, the tachyzoite loads in tissues were assessed, and animal survival time was recorded. Our results showed that the group immunised with 30 μg rTgPDI showed significantly higher levels of specific antibodies against the recombinant protein, a strong lymphoproliferative response and significantly higher levels of IgG2a, IFN-gamma (IFN-γ), IL-2 and IL-4 production compared with other doses and control groups. While no changes in IL-10 levels were detected. After being challenged with T. gondii tachyzoites, the numbers of tachyzoites in brain and liver tissues from the rTgPDI group were significantly reduced compared with those of the control group, and the survival time of the mice in the rTgPDI group was longer than that of mice in the control group. Our results showed that immunisation with rTgPDI elicited a protective immune reaction and suggested that rTgPDI might represent a promising vaccine candidate for combating toxoplasmosis.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Toxoplasma gondii Protein Disulfide Isomerase (TgPDI) Is a Novel Vaccine Candidate against Toxoplasmosis

Wang

Yin

et al. 2013

PLoS ONE

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“…Analysis of protein profiles is another way to search for variability and could be valuable for understanding biological processes including development, evolution and pathogenicity of these organisms. Proteomics involves the systematic analysis of gene expression at the protein level (Sperling 2001, Lee et al 2005). Proteomic techniques are powerful tools for comparing different isolates of the same species , Regidor-Cerrillo et al 2012 or morphospecies (Chan et al 2005), and assessing genetic variability (Mosquera et al 2003) and differences in virulence among isolates or strains of the same species (Regidor-Cerrillo et al 2012).…”

Section: Introductionmentioning

confidence: 99%

Cell proteome variability of protistan mollusc parasite Perkinsus olseni among regions of the Spanish coast

Fernández-Boo

Villalba²,

Cao³

2015

Dis. Aquat. Org.

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We evaluated the proteome variability of in vitro-cultured Perkinsus olseni cells deriving from 4 regions of the Spanish coast: the rías of Arousa and Pontevedra (Galicia, N W Spain), Carreras River in Huelva (Andalusia, SW Spain) and Delta de l'Ebre (Catalonia, N E Spain). P. olseni in vitro clonal cultures were produced starting from parasite isolates from 4 individual clams from each region. Those clonal cultures were used to extract cell proteins, which were separated by 2-dimensional (2D) electrophoresis. Qualitative comparison of P. olseni protein expression profiles among regions was performed with PD Quest software. Around 700 protein spots from parasites derived from each region were considered, from which 141 spots were shared by all the regions. Various spots were found to be exclusive to each region. Higher similarity was found among the proteomes of P. olseni from the Atlantic regions than between those from the Mediterranean and the Atlantic. A total of 54 spots were excised from the gels and sequenced. Nineteen proteins were annotated after searching in databases, 13 being shared by all the regions and 6 exclusive to 1 region. Most of the identified proteins were involved in glycolysis, oxidation/ reduction, metabolism and response to stress. No direct evidence of P. olseni variability associated with virulence was found within the protein set analysed, although the differences in metabolic adaptation and stress response could be connected to pathogenicity. KEY WORDS: Ruditapes decussatus · Ruditapes philippinarum · Mollusc · 2D electrophoresis · In vitro cultureResale or republication not permitted without written consent of the publisher

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“…Studies on proteomics have been carried out in certain diseases, including malignant tumors and neuropathy (13)(14)(15)(16)(17). A specific marker for a particular disease can be identified by comparing the protein spectrum of a patient with the disease with that of a healthy subject or with the protein spectrum from the gene bank (18).…”

Section: Introductionmentioning

confidence: 99%

Comparative analysis of serum proteomes: Identification of proteins associated with sciatica due to lumbar intervertebral disc herniation

et al. 2014

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Lumbar intervertebral disc herniation (LDH) is one of the most common orthopedic conditions that can cause lower back pain and sciatica. However, the pathogenesis of LDH is poorly understood. The aim of the present study was to use proteomic analysis of blood samples to establish whether there are serum proteins associated with LDH, which may be useful in elucidating LDH pathogenesis. The ultimate aim was to develop a simple technique for the diagnosis of LDH based on the blood samples of patients with sciatica. The study used comparative analysis of serum proteomes associated with sciatica due to LDH. A total of 30 LDH patients with sciatica, receiving treatment between August and December 2007, were selected as the experimental group (or LDH group). A total of 2 ml of blood was obtained from each of the 30 patients in the LDH group and from 30 healthy volunteers, who constituted the control group. Two-dimensional electrophoresis of the blood samples was conducted, distinct protein spots were identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and proteins associated with LDH were detected. An enzyme-linked immunosorbent assay (ELISA) was developed to screen for the LDH proteins and was tested on the sera of a second test and control group that included 10 patients with LDH and 10 healthy subjects, respectively. Based on signal intensity, the expression levels of 6 proteins on the dielectrophoretogram were found to be significantly associated with LDH. The identities of the LDH proteins were upregulated apolipoprotein-L1 (APO-L1) and two types of serum albumin precursors, and downregulated apolipoprotein M (APO-M), tetranectin (TN) and immunoglobulin light chain (IGL). Further ELISA experiments confirmed that there were increased serum levels of 4 out of the 6 proteins in patients with sciatica due to LDH, which was statistically different compared to the healthy subjects. In conclusion, these results suggest that serum APO-L1, TN, APO-M and IGL may serve as LDH biomarkers.

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Application of proteomics for comparison of proteome of Neospora caninum and Toxoplasma gondii tachyzoites

Cited by 30 publications

References 46 publications

Toxoplasma gondii Protein Disulfide Isomerase (TgPDI) Is a Novel Vaccine Candidate against Toxoplasmosis

Toxoplasma gondii Protein Disulfide Isomerase (TgPDI) Is a Novel Vaccine Candidate against Toxoplasmosis

Cell proteome variability of protistan mollusc parasite Perkinsus olseni among regions of the Spanish coast

Comparative analysis of serum proteomes: Identification of proteins associated with sciatica due to lumbar intervertebral disc herniation

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