Application of proteomics in biotechnology – Microbial proteomics

Josić, Djuro; Kovač, Spomenka

doi:10.1002/biot.200700234

Cited by 27 publications

(13 citation statements)

References 227 publications

(290 reference statements)

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“…After the structural and functional genomics "explosion", proteomics has become a powerful research tool in the discovery of new proteins and the understanding of complex biochemical processes and protein interactions [29]. Proteomics help us to know how, where, when, and what for are the several hundred thousands of individual protein species produced by a living organism or a complex microbial niche, being relevant to understand those systems where two or more players (i.e.…”

Section: Trichoderma Proteomicsmentioning

confidence: 99%

Proteomic Approaches to Understand Trichoderma Biocontrol Mechanisms and Plant Interactions

Hermosa¹,

Woo²,

Lorito³

et al. 2010

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Trichoderma is an ascomycete fungal genus including species with a significant impact on agriculture and industrial processes. The first application of the proteome technology was carried out to extract and separate cell wall associated proteins of T. reesei. Following this work several studies performed on the proteome of different strains of T. atroviride, T. asperellum or T. harzianum interacting with pathogens and plants have provided many novel data that improve our understanding of the agricultural and biotechnological value of these fungi.

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Section: Trichoderma Proteomicsmentioning

confidence: 99%

Proteomic Approaches to Understand Trichoderma Biocontrol Mechanisms and Plant Interactions

Hermosa¹,

Woo²,

Lorito³

et al. 2010

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“…In these cases, in addition to toxicity from the final compound, other factors such as the expression of numerous enzymes and imbalances in metabolic intermediates may also need to be identified. The value of proteomics and other cell wide studies has been well explored for microbial production of pharmaceuticals, enzyme and other such high value commodities [146][147][148][149]. Proteomic characterization of E. coli engineered to produce polyhydroxyalkanoates (PHA) revealed that central metabolic enzymes Eda, Fba and TpiA were over produced relative to wild type strains and likely to support increased acetyl-CoA and NADPH utilization [147].…”

Section: Endogenous Stressmentioning

confidence: 99%

The Role of Proteomics in the Development of Cellulosic Biofuels

Ito¹,

Kim²,

Mukhopadhyay³

et al. 2010

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Global demand on energy combined with dwindling fuel reserves has led to record fuel prices around the world and resulted in a concerted effort to identify alternate and sustainable fuel supplies. One such alternative is to produce cellulosic biofuels through the conversion of complex sugars found in plant cell walls (plant biomass) into fuels. While the synthesis of cellulosic biofuels is currently an achievable technology, associated production costs due to biomass recalcitrance, sugar composition and ineffectual conversion make their production impractical. In order to overcome these issues significant research will be required in areas ranging from plant cell wall biosynthesis, microbial host metabolism and tolerance that enable targeted engineering of these systems. Proteomics will play a central role in implementing this strategy by identifying new targets for biofuel crop engineering, analyzing engineered biochemical pathways and characterizing plant cell wall biosynthesis. This review will examine the current use of proteomics to fast-track cellulosic biofuel production and evaluate the potential of this technology to provide significant breakthroughs in this area.

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“…However, they still contain relatively high amounts of other, co-fractionating proteins, and in contrast to their name, most impurities are still biochemically poorly characterized or not identified at all [3,4]. The idea to use proteomics for optimization of the production of therapeutic plasma proteins was proposed more than 7 years ago, when the terms ''industrial proteomics'' and ''industrial-scale proteomics'' were also coined [5,6]. Proteomic techniques are successfully used in the design and optimization of bioprocesses; the characterization of downstream processing; scale up and process characterization and validation [6,7].…”

Section: Introductionmentioning

confidence: 99%

Therapeutic plasma proteins – application of proteomics in process optimization, validation, and analysis of the final product

et al. 2011

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An overview is given on the application of proteomic technology in the monitoring of different steps during the production of therapeutic proteins from human plasma. Recent advances in this technology enable the use of proteomics as an advantageous tool for the validation of already existing processes, the development and fine tuning of new production steps, the characterization and quality control of final products, the detection of both harmful impurities and modifications of the therapeutic protein and the auditing of batch-to-batch variations. Further, use of proteomics for preclinical testing of new products, which can be either recombinant or plasma-derived, is also discussed.

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Application of proteomics in biotechnology – Microbial proteomics

Cited by 27 publications

References 227 publications

Proteomic Approaches to Understand Trichoderma Biocontrol Mechanisms and Plant Interactions

Proteomic Approaches to Understand Trichoderma Biocontrol Mechanisms and Plant Interactions

The Role of Proteomics in the Development of Cellulosic Biofuels

Therapeutic plasma proteins – application of proteomics in process optimization, validation, and analysis of the final product

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