Application of pulsed-magnetic field enhances non-viral gene delivery in primary cells from different origins

Chapman, Sarah; Hassa, Paul O.; Koch-Schneidemann, Sabine; Rechenberg, Brigitte von; Hofmann-Amtenbrink, Margarethe; Steitz, Benedikt; Petri‐Fink, Alke; Hofmann, Heinrich; Hottiger, Michael O.

doi:10.1016/j.jmmm.2008.01.002

Cited by 22 publications

(18 citation statements)

References 70 publications

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“…red fluorescent protein ( DsRed) reporter genes to various cell lines, however, to a lesser extent, i.e. from 3-fold to 36-fold 116–119…”

Section: Applications Of Magnetic Nanoparticles In Oncologymentioning

confidence: 99%

“…In another study of the same group, at least a 6-fold increase in transfection efficiency of the EGFP reporter gene to various primary cell lines was shown when a combination of static and pulsating magnetic field was used compared to the presence of static magnetic field alone. The transfection was the lowest when the cells were exposed only to the pulsed magnetic field 119. On the other hand, the American group utilized a computer-controlled stepper motor-driven horizontally oscillating magnet array system which produced increased magnetic field strength and a gradient with no heating in comparison to electromagnets used by the Swiss group.…”

Section: Applications Of Magnetic Nanoparticles In Oncologymentioning

confidence: 99%

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Magnetic nanoparticles as targeted delivery systems in oncology

Prijic¹,

Serša²

2011

Radiology and Oncology

179

117

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BackgroundMany different types of nanoparticles, magnetic nanoparticles being just a category among them, offer exciting opportunities for technologies at the interfaces between chemistry, physics and biology. Some magnetic nanoparticles have already been utilized in clinical practice as contrast enhancing agents for magnetic resonance imaging (MRI). However, their physicochemical properties are constantly being improved upon also for other biological applications, such as magnetically-guided delivery systems for different therapeutics. By exposure of magnetic nanoparticles with attached therapeutics to an external magnetic field with appropriate characteristics, they are concentrated and retained at the preferred site which enables the targeted delivery of therapeutics to the desired spot.ConclusionsThe idea of binding chemotherapeutics to magnetic nanoparticles has been around for 30 years, however, no magnetic nanoparticles as delivery systems have yet been approved for clinical practice. Recently, binding of nucleic acids to magnetic nanoparticles has been demonstrated as a successful non-viral transfection method of different cell lines in vitro. With the optimization of this method called magnetofection, it will hopefully become another form of gene delivery for the treatment of cancer.

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“…red fluorescent protein ( DsRed) reporter genes to various cell lines, however, to a lesser extent, i.e. from 3-fold to 36-fold 116–119…”

Section: Applications Of Magnetic Nanoparticles In Oncologymentioning

confidence: 99%

Section: Applications Of Magnetic Nanoparticles In Oncologymentioning

confidence: 99%

Magnetic nanoparticles as targeted delivery systems in oncology

Prijic¹,

Serša²

2011

Radiology and Oncology

179

117

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“…32 Although MF demonstrated dose-dependent toxicity on cells, using suitable MP:DNA ratios and proper magnetic field intensities/exposure times can decrease the detrimental impacts of MF on cells, 33 resulting in successful transfection to stem cells and other difficult-to-transfect cells such as human endothelial cells, 29,34,35 neural stem cells, 36,37 neurons, 38,39 and other primary cell types. 40 …”

Section: Introductionmentioning

confidence: 99%

Magnetofection Mediated Transient NANOG Overexpression Enhances Proliferation and Myogenic Differentiation of Human Hair Follicle Derived Mesenchymal Stem Cells

Son¹,

Liang²,

Lei³

et al. 2015

Bioconjugate Chem.

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We used magnetofection (MF) to achieve high transfection efficiency into human mesenchymal stem cells (MSCs). A custom-made magnet array, matching well-to-well to a 24-well plate, was generated and characterized. Theoretical predictions of magnetic force distribution within each well demonstrated that there was no magnetic field interference among magnets in adjacent wells. An optimized protocol for efficient gene delivery to human hair follicle derived MSCs (hHF-MSCs) was established using an egfp-encoding plasmid, reaching approximately ~50% transfection efficiency without significant cytotoxicity. Then we applied the optimized MF protocol to express the pluripotency-associated transcription factor NANOG, which was previously shown to reverse the effects of organismal aging on MSC proliferation and myogenic differentiation capacity. Indeed, MF-mediated NANOG delivery increased proliferation and enhanced the differentiation of hHF-MSCs into smooth muscle cells (SMCs). Collectively, our results show that MF can achieve high levels of gene delivery to MSCs and, therefore, may be employed to moderate or reverse the effects of cellular senescence or reprogram cells to the pluripotent state without permanent genetic modification.

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“…In general, the transfection efficiency of nonviral gene delivery systems in non-dividing primary cells like DCs decreases considerably when compared with transformed cell lines. 8 A study recently done by Chapman et al 9 found that the application of permanent and pulsating magnetic fields could significantly enhance the efficiency of gene delivery in primary cells such as macrophages, synoviocytes, chondrocytes and others isolated from different organs if PEI-coated magnetic nanoparticles were used.…”

Section: Introductionmentioning

confidence: 99%

Design of magnetic polyplexes taken up efficiently by dendritic cell for enhanced DNA vaccine delivery

et al. 2013

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Dendritic cells (DC) targeting vaccines require high efficiency for uptake, followed by DC activation and maturation. We used magnetic vectors comprising polyethylenimine (PEI)-coated superparamagnetic iron oxide nanoparticles, with hyaluronic acid (HA) of different molecular weights (<10 and 900 kDa) to reduce cytotoxicity and to facilitate endocytosis of particles into DCs via specific surface receptors. DNA encoding Plasmodium yoelii merozoite surface protein 1-19 and a plasmid encoding yellow fluorescent gene were added to the magnetic complexes with various % charge ratios of HA: PEI. The presence of magnetic fields significantly enhanced DC transfection and maturation. Vectors containing a high-molecular-weight HA with 100% charge ratio of HA: PEI yielded a better transfection efficiency than others. This phenomenon was attributed to their longer molecular chains and higher mucoadhesive properties aiding DNA condensation and stability. Insights gained should improve the design of more effective DNA vaccine delivery systems.

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Application of pulsed-magnetic field enhances non-viral gene delivery in primary cells from different origins

Cited by 22 publications

References 70 publications

Magnetic nanoparticles as targeted delivery systems in oncology

Magnetic nanoparticles as targeted delivery systems in oncology

Magnetofection Mediated Transient NANOG Overexpression Enhances Proliferation and Myogenic Differentiation of Human Hair Follicle Derived Mesenchymal Stem Cells

Design of magnetic polyplexes taken up efficiently by dendritic cell for enhanced DNA vaccine delivery

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