Application of quartz tuning forks for detection of endotoxins and Gram-negative bacterial cells by monitoring of Limulus Amebocyte Lysate coagulation

Chałupniak, Andrzej; Waszczuk, K.; Halubek-Gluchowska, Katarzyna; Piasecki, Tomasz; Gotszalk, Teodor; Rybka, Jacek

doi:10.1016/j.bios.2014.02.048

Cited by 21 publications

(6 citation statements)

References 27 publications

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“…Lipopolysaccharide (LPS) detection in the exosomes LPS in the P. aeruginosa-derived exosomes was detected by Limulus Amebocyte Lysate (LAL) test according to the previous study 17 in Supplemental Table S1.…”

Section: Animalsmentioning

confidence: 99%

Pseudomonas aeruginosa-derived exosomes ameliorates allergic reactions via inducing the Treg response in asthma

et al. 2018

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Section: Animalsmentioning

confidence: 99%

Pseudomonas aeruginosa-derived exosomes ameliorates allergic reactions via inducing the Treg response in asthma

et al. 2018

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“…Table 1 shows the comparison of our results with the methods used in the industry as well as those new approaches under development by different laboratories. [15][16][17]19,20,31 The significant improvement of our approach in detection sensitivity can be attributed to the PC-TIR's capability for ultrasensitive measurements of the RI changes in the analyte solutions caused by LAL reactions to the minute amount of endotoxin. The sharp resonance bandwidth (2 nm) of the PC-TIR sensor shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Due to the limitations of the current LAL test, as well as the increasing demand of endotoxin testing in the pharmaceutical industry, refinements of pyrogen tests, including using Quartz sensors, 15,16 electrochemical feedback sensors, 17 nanoparticle suspensions, 18 piezoelectric sensors, 19 and surface-plasmonresonance (SPR)-based sensors, 20 are constantly being made. In addition, alternative pyrogen test compounds, such as the beta glucan only test, Glucatell, 21 the monoclonal antibody test, 3,22 recombinant factor C, 4 and nanomolecular imprinted polymers, 20 are constantly being engineered.…”

Section: Introductionmentioning

confidence: 99%

Limulus amoebocyte lysate test via an open-microcavity optical biosensor

Scudder

2018

J. Biomed. Opt.

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Almost since its discovery, Limulus amoebocyte lysate (LAL) testing has been an important part of the pharmaceutical quality control toolkit. It allows for in vitro endotoxin testing, which has replaced tests using animals, such as using rabbits' thermal response to judge pyrogenicity of test samples, thus leading to a less expensive and faster test of parenteral pharmaceuticals and medical devices that contact blood or cerebrospinal fluid. However, limited by the detection mechanisms of the LAL assays currently used in industry, further improvement in their performance is challenging. To address the growing demand on optimizing LAL assays for increased test sensitivity and reduced assay time, we have developed an LAL assay approach based on a detection mechanism that is different from those being used in industry, namely, gel-clot, turbidimetric, and chromogenic detection. Using a unique open-microcavity photonic-crystal biosensor to monitor the change in the refractive index due to the reaction between LAL regents and endotoxins, we have demonstrated that this approach has improved the LAL assay sensitivity by 200 times compared with the commercial standard methods, reduced the time needed for the assay by more than half, and eliminated the necessity to incubate the test samples. This study opens up the possibility of using the significantly improved LAL assays for a wide range of applications.

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“…The fourth setup example that can be found in some QTF studies are specially designed devices which consist of a function generator and again some analog circuits like transimpedance amplifier, voltage amplifier, filters. [32]. Moreover, there are also some more additional circuits which may be analog or digital like windowing, peak detector, zero crossing.…”

Section: Introductionmentioning

confidence: 99%

Design of a portable and low-cost mass-sensitive sensor with the capability ofmeasurements on various frequency quartz tuning forks

Ünal¹,

Kocum²,

Serdaroğlu³

2019

Turk J Elec Eng & Comp Sci

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Recently, sensor and biosensor applications have become widespread and are now significant tools in the biomedical field and other areas. Since quartz tuning fork (QTF) resonance frequency depends on the mass adsorbed to its prongs, it is generally used to measure minor mass change and detect target analyte in picogram levels. This study is undertaken to design and fabricate a sensor device for the measurement of QTF transducers. When QTF sensor studies were investigated, it was found that explanations on the details of instrumentation part were limited, and in addition, there was no compact commercial products. In this study, a novel, low-cost, portable quartz tuning fork sensor device with ability to work with any kind of display is presented in detail. Moreover, the effect of the ambient temperature during the measurements was checked. Six other QTF transducer types, 32, 32.768, 40, 65.536, 75, and 100 kHz, have been studied as the other novel part of the research. As a result, a QTF sensor device was fabricated that has further advanced features when compared with its alternatives and works with a more accurate measurement method. Owing to this advancement, using biosensor/chemical sensor which consists of QTF transducers with features that can take sensitive analyte measurement in picogram level will be able to spread.

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Application of quartz tuning forks for detection of endotoxins and Gram-negative bacterial cells by monitoring of Limulus Amebocyte Lysate coagulation

Cited by 21 publications

References 27 publications

Pseudomonas aeruginosa-derived exosomes ameliorates allergic reactions via inducing the Treg response in asthma

Pseudomonas aeruginosa-derived exosomes ameliorates allergic reactions via inducing the Treg response in asthma

Limulus amoebocyte lysate test via an open-microcavity optical biosensor

Design of a portable and low-cost mass-sensitive sensor with the capability ofmeasurements on various frequency quartz tuning forks

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