Application of quinidine on rat sciatic nerve decreases the amplitude and increases the latency of evoked responses

Cheng, Kuang‐I; Lin, I‐Ling; Chang, Lin‐Li; Jou, I‐Ming; Lai, Chung-Sheng; Wang, Jhi-Joung; Wang, Hung-Chen; Kwan, Aij‐Lie

doi:10.1007/s00540-013-1754-x

Cited by 3 publications

(1 citation statement)

References 22 publications

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“…These results are in agreement with data on human subjects, and partially, with their lipid solubility; i.e., the octanol/buffer partition coefficient of lidocaine is the lowest, which might lead to its lower potency (Strichartz et al, 1990). Since other class 1 antiarrhythmic drugs such as quinidine also inhibited the evoked compound muscle action potentials, we suppose that our model might be capable to display some intermediate degree of actions mediated by Na v s (Cheng et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

In vivo potency of different ligands on voltage-gated sodium channels

Safrany-Fark

Petrovszki

Kekesi

et al. 2015

European Journal of Pharmacology

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The Ranvier nodes of thick myelinated nerve fibers contain almost exclusively voltage-gated sodium channels (Navs), while the unmyelinated fibers have several receptors (e.g., cannabinoid, transient receptor potential vanilloid receptor 1), too. Therefore, a nerve which contains only motor fibers can be an appropriate in vivo model for selective influence of Navs. The goals were to evaluate the potency of local anesthetic drugs on such a nerve in vivo; furthermore, to investigate the effects of ligands with different structures (arachidonic acid, anandamide, capsaicin and nisoxetine) that were proved to inhibit Navs in vitro with antinociceptive properties. The marginal mandibular branch of the facial nerve was explored in anesthetized Wistar rats; after its stimulation, the electrical activity of the vibrissae muscles was registered following the perineural injection of different drugs. Lidocaine, bupivacaine and ropivacaine evoked dose-dependent decrease in electromyographic activity, i.e., lidocaine had lower potency than bupivacaine or ropivacaine. QX-314 did not cause any effect by itself, but its co-application with lidocaine produced a prolonged inhibition. Nisoxetine had a very low potency. While anandamide and capsaicin in high doses caused about 50% decrease in the amplitude of action potential, arachidonic acid did not influence the responses. We proved that the classical local anesthetics have high potency on motor nerves, suggesting that this method might be a reliable model for selective targeting of Navs in vivo circumstances. It is proposed that the effects of these endogenous lipids and capsaicin on sensory fibers are not primarily mediated by Navs.

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Section: Discussionmentioning

confidence: 99%