Application of Quorum Quenching to Inhibit Biofilm Formation

Paul, Diby; Kim, Young Sam; Ponnusamy, Kannan; Kweon, Ji Hyang

doi:10.1089/ees.2008.0392

Cited by 83 publications

(50 citation statements)

References 33 publications

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“…The activity of the coating was monitored spectrophotometrically via titrating the liberation of primary amines with TNBS. While N-acetyl-L-methionine is not a quorum sensing substrate, N-butyryl-L-homoserine lactone is used by bacteria for QS (Paul et al, 2009;Vikstr€ om et al, 2006). Although the activity retention of the immobilized enzyme was low (5% as measured by the more sensitive fluorescence assay), the retained activity was comparable to that for other enzymes that were similarly immobilized via multipoint covalent attachment in waterborne polyurethane coatings (Drevon and Russell, 2000).…”

Section: Preparation and Activity Of Acylase-containing Coatingsmentioning

confidence: 95%

“…Similar results were reported by Sio et al (2006), albeit with a different acylase, who found that acylase from P. aeruginosa PAO1 was inactive against C4-LHL and C6-LHL, but highly active against 3-oxo-C12-LHL. Notably, C4-LHL and 3-oxo-C12-LHL are the primary QS molecules responsible for the formation of biofilms by P. aeruginosa (Paul et al, 2009;Singh et al, 2000;Sio et al, 2006;Vikstr€ om et al, 2006), making the activity of the coatings towards these substrates critical.…”

Section: Degradation Of Qs Moleculesmentioning

confidence: 99%

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Acylase‐containing polyurethane coatings with anti‐biofilm activity

Grover

Plaks

Summers

et al. 2016

Biotech & Bioengineering

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Due to the prevalence of biofilm-related infections, which are mediated by bacterial quorum sensing, there is a critical need for materials and coatings that resist biofilm formation. We have developed novel anti-biofilm coatings that disrupt quorum sensing in surface-associated bacteria via the immobilization of acylase in polyurethane films. Specifically, acylase from Aspergillus melleus was covalently immobilized in biomedical grade polyurethane coatings via multipoint covalent immobilization. Coatings containing acylase were enzymatically active and catalyzed the hydrolysis of the quorum sensing (QS) molecules N-butyryl-L-homoserine lactone (C4-LHL), N-hexanoyl-L-homoserine lactone (C6-LHL), and N-(3-oxododecanoyl)-L-homoserine lactone (3-oxo-C12-LHL). In biofilm inhibition assays, immobilization of acylase led to an approximately 60% reduction in biofilm formation by Pseudomonas aeruginosa ATCC 10145 and PAO1. Inhibition of biofilm formation was consistent with a reduction in the secretion of pyocyanin, indicating the disruption of quorum sensing as the mechanism of the coating activity. Scanning electron microscopy further showed that acylase-containing coatings contained far fewer bacterial cells than control coatings that lacked acylase. Moreover, acylase-containing coatings retained 90% activity when stored dry at 37°C for 7 days and were more stable than the free enzyme in physiological conditions, including artificial urine. Ultimately, such coatings hold considerable promise for the clinical management of catheter-related infections as well as the prevention of infections in orthopedic applications (i.e., on hip and knee prostheses) and on contact lenses. Biotechnol. Bioeng. 2016;113: 2535-2543. © 2016 Wiley Periodicals, Inc.

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Section: Preparation and Activity Of Acylase-containing Coatingsmentioning

confidence: 95%

Section: Degradation Of Qs Moleculesmentioning

confidence: 99%

Acylase‐containing polyurethane coatings with anti‐biofilm activity

Grover

Plaks

Summers

et al. 2016

Biotech & Bioengineering

View full text Add to dashboard Cite

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“…[23][24][25] QS enzymes had also been found in some animals such as mice, rats and zebrafish. [26] In addition, plant extracts had been found to act as QSIs because of their similar chemical structure to those of QS signals and also because of their ability to degrade signal receptors. [27,28] Although naturally occurring QSIs had been widely reported, the low concentrations in organisms limited their application in the environment.…”

Section: Discussionmentioning

confidence: 99%

The influence of bacterial quorum-sensing inhibitors against the formation of the diatom-biofilm

Yang

Song

Zhu

et al. 2016

Chemistry and Ecology

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Quorum-sensing inhibitor (QSI) is one of the most promising and environmentally friendly agents for marine antifouling. In this study, the activities of three kinds of QSIs 3,4-dibromo-2(5)Hfuranone, 4-nitropyridine-N-oxide and indole were evaluated on the growth of two marine diatoms Cylinthrotheca sp. and Nitzschia closterium. At the same time, the effects of QSIs on the formation of the diatom-biofilm were also discussed. All the results showed that QSIs significantly inhibited the growth, and the effects depended on the dose and diatom species. The extracellular polymeric substance contents in the diatom-biofilm were significantly reduced by QSIs. However, the contents of polysaccharide in culture mediums were increased, which might result in the destruction of diatom cells. Combined with the results of crystal violet staining-biofilm and images of scanning electron microscopy, it was further demonstrated that QSIs inhibited the biofilm formation of Cylindrotheca sp., and the inhibitory effect of 4-nitropyridine-N-oxide was superior to that of 3,4-dibromo-2(5)H-furanone and indole.ARTICLE HISTORY

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“…Kim et al [38] observed the occurrence of AHL and AI-2 from the fouled reverse osmosis membrane. Paul et al [107] found that biofilm formation by bacterial strains A. hydrophila and Pseudomonas putida isolated from fouled RO membrane was reduced with the addition of acylase 1 at a concentration of 60 g/ml. Paul et al [107] further observed gradual decrease in biofilm with increasing concentration (5-60 g/ml) of acylase 1.…”

Section: Degradation By Free Enzymesmentioning

confidence: 99%