Application of real-time PCR, DGGE fingerprinting, and culture-based method to evaluate the effectiveness of intrinsic bioremediation on the control of petroleum-hydrocarbon plume

Kao, C. L.; Chen, Colin S.; Tsa, Fu Yu; Yang, Kai; Chien, Chih-Ching; Liang, Shih Hsiung; Yang, Chin an; Chen, Ssu Ching

doi:10.1016/j.jhazmat.2010.01.096

Cited by 50 publications

(23 citation statements)

References 25 publications

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“…A higher concentration of methane was also detected in the area with a higher concentration of CO 2 , reflecting that mixed anaerobic biodegradation process occurred in this area (Kao et al, 2010). Moreover, high CO 2 concentrations were observed in the plume, which suggests that significant microbial activity and natural bioremediation occurred in this area.…”

Section: Evaluation Of Intrinsic Remediation By Realtime Pcrmentioning

confidence: 76%

“…The purpose of this paper is to provide a review of the past work performed in the petroleum contaminated site (Kao et al, 2010;Chen et al, 2011). A series of study include the following tasks: (1) …”

Section: Toxic Chemical Substances By Taiwan Environmentalmentioning

confidence: 99%

“…The measured groundwater temperature in the surficial aquifer varies from 17 to 29 o C. Fig. 1 illustrates the locations of representative monitor wells, and groundwater flow direction in the early stage of the studies (Kao et al, 2010). Groundwater samples were collected from four representative monitor wells CT-2, CT-4, which were located at the background area, source area, midplume area, and down gradient area, respectively.…”

Section: Gasoline Contaminated Sitementioning

confidence: 99%

“…It also provided a baseline to evaluate the impact of some strategies regarding enhanced bioremediation including the injection of oxygen-releasing materials (ORMs), multi-phase extraction, or in situ biosparging (Kao et al, 2010).…”

Section: Evaluation Of Intrinsic Remediation By Realtime Pcrmentioning

confidence: 99%

“…A phylogenetic tree of these sequences was constructed in order to describe the relationships between all the sequences (Kao et al, 2010 Li t al., 2002). Thus, the dominant microorganisms involving petroleum degradation could be exploited and isolated for their application on the bioremediation of gasoline-contaminated sites.…”

Section: The Bacterial Community At the Contaminated Sitementioning

confidence: 99%

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Evaluation of Intrinsic Bioremediation of Methyl Tert-butyl Ether (MTBE) Contaminated Groundwater

Chen¹,

Tien²,

Zhan³

2014

Journal of Soil and Groundwater Environment

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This paper reported the use of real-time polymerase chain reaction (PCR), denaturing gradient gel electrophoresis (DGGE), and the culture-based method in the intrinsic bioremediation study at a petroleum contaminated site. The study showed that phenol hydroxylase gene was detected in groundwater contaminated with benzene, toluene, ethylbenzene, xylene isomers (BTEX) and methyl tert-butyl ether (MTBE). This indicated that intrinsic bioremediation occurred at the site. DGGE analyses revealed that the petroleum-hydrocarbon plume caused the variation in microbial communities. MTBE degraders including Pseudomonas sp. NKNU01, Bacillus sp. NKNU01, Klebsiella sp. NKNU01, Enterobacter sp. NKNU01, and Enterobacter sp. NKNU02 were isolated from the contaminated groundwater using the cultured-based method. Among these five strains, Enterobacter sp. NKNU02 is the most effective stain at degrading MTBE without the addition of pentane. The MTBE biodegradation experiment indicated that the isolated bacteria were affected by propane. Biodegradation of MTBE was decreased but not totally inhibited in the mixtures of BTEX. Enterobacter sp. NKNU02 degraded about 60% of MTBE in the bioreactor study. Tert-butyl alcohol (TBA), acetic acid, 2-propanol, and propenoic acid were detected using gas chromatography/mass spectrometry during MTBE degraded by the rest cells of Enterobacter sp. NKNU02. The effectiveness of bioremediation of MTBE was assessed for potential field-scale application.

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Section: Evaluation Of Intrinsic Remediation By Realtime Pcrmentioning

confidence: 76%

Section: Toxic Chemical Substances By Taiwan Environmentalmentioning

confidence: 99%

Section: Gasoline Contaminated Sitementioning

confidence: 99%

Section: Evaluation Of Intrinsic Remediation By Realtime Pcrmentioning

confidence: 99%

Section: The Bacterial Community At the Contaminated Sitementioning

confidence: 99%

See 3 more Smart Citations

Evaluation of Intrinsic Bioremediation of Methyl Tert-butyl Ether (MTBE) Contaminated Groundwater

Chen¹,

Tien²,

Zhan³

2014

Journal of Soil and Groundwater Environment

Self Cite

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An In Situ Bioreactor for the Treatment of Petroleum Hydrocarbons in Groundwater

Key

Sublette

Johannes

et al. 2013

Remediation Journal

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Two pilot tests of an aerobic in situ bioreactor (ISBR) have been conducted at field sites contaminated with petroleum hydrocarbons. The two sites differed with respect to hydrocarbon concentrations. At one site, concentrations were low but persistent, and at the other site concentrations were high enough to be inhibitory to biodegradation. The ISBR unit is designed to enhance biodegradation of hydrocarbons by stimulating indigenous microorganisms. This approach builds on existing Bio-Sep ® bead technology, which provides a matrix that can be rapidly colonized by the active members of the microbial community and serves to concentrate indigenous degraders.Oxygen and nutrients are delivered to the bioreactor to maintain conditions favorable for growth and reproduction, and contaminated groundwater is treated as it is circulated through the bed of Bio-Sep ® beads. Groundwater moving through the system also transports degraders released from Bio-Sep ® beads away from the bioreactor, potentially increasing biodegradation rates throughout the aquifer.Groundwater sampling, Bio-Traps, and molecular biological tools were used to assess ISBR performance during the two pilot tests. Groundwater monitoring indicated that contaminant concentrations decreased at both sites, and the microbial data suggested that these decreases were due to degradation by indigenous microorganisms rather than dilution or dispersion mechanisms.Taken together, these lines of evidence showed that the ISBR system effectively increased the number and activity of indigenous microbial degraders and enhanced bioremediation at the test sites. O

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Indigenous PAH-Degrading Bacteria in Oil-Polluted Marine Sediments from Patagonia: Diversity and Biotechnological Properties

Isaac

Bourguignon

Maizel

et al. 2016

Biology and Biotechnology of Patagonian Microorganisms

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Application of real-time PCR, DGGE fingerprinting, and culture-based method to evaluate the effectiveness of intrinsic bioremediation on the control of petroleum-hydrocarbon plume

Cited by 50 publications

References 25 publications

Evaluation of Intrinsic Bioremediation of Methyl Tert-butyl Ether (MTBE) Contaminated Groundwater

Evaluation of Intrinsic Bioremediation of Methyl Tert-butyl Ether (MTBE) Contaminated Groundwater

An In Situ Bioreactor for the Treatment of Petroleum Hydrocarbons in Groundwater

Indigenous PAH-Degrading Bacteria in Oil-Polluted Marine Sediments from Patagonia: Diversity and Biotechnological Properties

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