Application of real-time PCR for detection of Lawsonia intracellularis and Brachyspira hyodysenteriae in fecal samples from pigs

Żmudzki, J.; Szczotka, A.; Podgórska, Katarzyna; Nowak, Agnieszka; Grzesiak, Anna; Dors, Arkadiusz; Pejsak, Z.

doi:10.2478/v10181-011-0144-2

Cited by 9 publications

(5 citation statements)

References 13 publications

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“…In terms of fecal samples, 7/162 (4.32%) were positive by qPCR, from 4/20 (20%) of the evaluated farms (Table 2). Zmudzki et al (2012) investigated the efficiency of qPCR, using the same primer as the present study, and found good sensitivity and specificity values in the detection of L. intracellularis in swine feces, demonstrating the possibility of using the technique in the diagnosis of proliferative enteropathy. These animals, which were positive in molecular biology, originated from four out of the seven (4/7) farms with positive serology, indicating the circulation of the agent on them.…”

Section: Resultsmentioning

confidence: 53%

Occurrence of Lawsonia intracellularis in horses raised in three regions of the state of Paraná, Brazil

Caleffo

Dahm

Santos

et al. 2021

Semina: Ciênc. Agrár.

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Lawsonia intracellularis is a bacterium already described in several species and most prevalent in pigs, in which it causes enteric problems. Horses can also be affected, developing a disease known as equine proliferative enteropathy, which results from the proliferation of intestinal crypt cells in response to infection by the bacterium. Despite the existence of reports of the disease in several countries, including Brazil, there are still no reports of the disease or epidemiological studies of its occurrence in symptomatic or asymptomatic horses in the state of Paraná. Thus, the present study was conducted to examine the occurrence of L. intracellularis in asymptomatic horses raised in the west, northwest and north regions of Paraná by means of serological testing and the real-time polymerase chain reaction (qPCR) technique. In the serological approach, the immunoperoxidase monolayer assay (IPMA) technique was employed. Feces were processed and subjected to qPCR. In total, samples were collected from 162 animals from 20 farms. Of these, 9/162 (5.55%) showed specific antibodies against L. intracellularis. Real-time PCR, on the other hand, identified 7/162 (4.32%) fecal samples positive for the presence of the bacterium. When the techniques were compared, none of the samples was positive by both, demonstrating that, for a better diagnosis, they must be performed together. In contrast to most reports in horses, the present study describes higher serological and molecular occurrence in animals older than two years. These results are of great epidemiological relevance, as they indicate that the bacterium is present in the sampled regions of the state of Paraná. Therefore, the disease must be included in the differential diagnosis of diseases with similar clinical manifestations.

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Section: Resultsmentioning

confidence: 53%

Occurrence of Lawsonia intracellularis in horses raised in three regions of the state of Paraná, Brazil

Caleffo

Dahm

Santos

et al. 2021

Semina: Ciênc. Agrár.

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“…Pooled fecal samples were homogenized in single-use plastic containers and total genomic DNA was extracted using a commercial isolation kit (Genomic Mini, A&A Biotechnology, Gdynia, Poland), according to the manufacturer's recommenda- Real-time PCR amplification of total DNA extracted from fecal samples for detection of L. intracellularis and B. hyodysenteriae was carried out according to the method described previously (Zmudzki et al 2012).…”

Section: Pcr Techniquesmentioning

confidence: 99%

Prevalence and risk factors for Lawsonia intracellularis, Brachyspira hyodysenteriae and Salmonella spp. in finishing pigs in Polish farrow-to-finish swine herds

Dors¹,

Pomorska‐Mól²,

Czyzewska³

et al. 2015

Polish Journal of Veterinary Sciences

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The aim of the study was to estimate the herd-level, within-herd prevalence, the frequency of mixed infections and risk factors for L. intracellularis, B. hyodysenteriae and Salmonella spp. in selected farrow-to-finish Polish pig herds. A total of 254 pooled fecal samples were collected from 9 to 24 week-old pigs in 70 herds. Real time PCR for detection of L. intracellularis and B. hyodysenteriae was performed. For Salmonella spp. bacteriological examination was performed. The herd-level prevalences of L. intracellularis, B. hyodysenteriae and Salmonella spp. among examined herds were 65.7%, 1.4% and 8.6%, respectively. The within-herd prevalences (in positive herds) for L. intracellularis, B. hyodysenteriae and Salmonella spp. were 51.5%, 75.0% and 30.4%, respectively. All herds with diarrhea observed during sampling were infected with L. intracellularis and 60% of herds with no diarrhea at the moment of sampling were infected with L. intracellularis (p=0.035). In herds with more than 200 sows the prevalence of Salmonella spp. was significantly higher compared to herds with less than 200 sows (p=0.027). In herds where all-in/all-out (AIAO) was respected, prevalence of L. intracellularis was significantly lower than in herds where this rule was not kept (p=0.024). Obtained results confirm that L. intracellularis is the major cause of bacterial diarrhea in finishing pigs. The present study identified AIAO and herd size as a risk factor, at the herd level, for L. intracellularis and Salmonella spp., respectively.

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“…Next, slides were analysed by in situ hybridization (ISH) for PCV2 identification ( 16 ) and were haematoxylin and eosin stained for histopathological examination. The faeces and scrapings were subjected to a real-time PCR for identification of Lawsonia intracellularis and Brachyspira hyodysenteriae ( 25 ). Additionally, parasitological analyses of faeces and intestinal content collected from the colon were performed with the use of flotation in a saturated NaCl solution supplemented with sugar with specific gravity of 1.3 ( 22 ) and with decantation ( 24 ).…”

Section: Methodsmentioning

confidence: 99%

Balantidium coli in pig farms suspected of porcine circovirus type 2 (PCV2) associated enteritis

Szczotka-Bochniarz

Karamon

Nowak

et al. 2021

Journal of Veterinary Research

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Introduction Diarrhoea in growing-finishing pigs is a common problem of commercial pig farms. Among many causative factors, porcine circovirus type 2 (PCV2) is one considered an important pathogen in modern pig production. The aim of the study was to verify if PCV2 was responsible for antibiotic non-responsive diarrhoea and wasting in pigs. Material and Methods A total of 13 dead pigs aged between 12 and 15 weeks from three Polish farms with persistent herd symptoms suggestive of PCV2 infection were provided for evaluation. Sections of lymph nodes and intestines were analysed by in situ hybridization (ISH) for PCV2 and histopathological examination. Faeces and intestinal scrapings were tested for Lawsonia intracellularis and Brachyspira hyodysenteriae by real-time PCR and for parasitic infection by flotation and decantation. Results ISH and histopathological examination showed that all pigs were PCV2 systemic disease negative. Swine dysentery was confirmed by real-time PCR on two farms, and proliferative enteropathy on one farm. In histological examinations, erosions of the caecal and colonic mucosa were found, together with cysts and trophozoites of Balantidium coli. The protozoa were present in the intestinal lumen and mucosa. B. coli cysts were identified in faeces from all examined pigs. Conclusion These results suggest that monitoring of B. coli infections should be an additional measure of control and prevention of gastrointestinal tract disorders in modern swine husbandry.

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Application of real-time PCR for detection of Lawsonia intracellularis and Brachyspira hyodysenteriae in fecal samples from pigs

Cited by 9 publications

References 13 publications

Occurrence of Lawsonia intracellularis in horses raised in three regions of the state of Paraná, Brazil

Occurrence of Lawsonia intracellularis in horses raised in three regions of the state of Paraná, Brazil

Prevalence and risk factors for Lawsonia intracellularis, Brachyspira hyodysenteriae and Salmonella spp. in finishing pigs in Polish farrow-to-finish swine herds

Balantidium coli in pig farms suspected of porcine circovirus type 2 (PCV2) associated enteritis

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