Background: Dharoi Reservoir, located in Gujarat, India, is a vital freshwater resource supporting agriculture, industry, and local communities. Chl-a, a key indicator of water quality, reflects the trophic state and ecological balance of aquatic systems. Objective(s): This study aims to provide comprehensive insights into the water quality dynamics of Dharoi Reservoir, offering valuable information for environmental management and sustainable water resource planning. Methods: This study employs high-resolution Sentinel-2 satellite imagery to analyze Chl-a concentrations in the reservoir during October 2020. The Chl-a index, calculated by dividing Sentinel-2 bands B5 and B4, reveals a spatial distribution of Chl-a concentrations. Results: The Chl-a index ranges from 73.78 to 100. The mean Chl-a index is 91.6 with a standard deviation of 3.27, indicating elevated and variable Chl-a concentrations. Conclusions: The findings contribute to the understanding of the reservoir’s ecological health and assist in making informed decisions for water quality management. This research exemplifies the integration of remote sensing technology and environmental stewardship, promoting sustainable water management practices in the region. Policy recommendations: No policy recommendations are explicitly stated in the abstract, but they could be inferred from the conclusions. For example, one possible policy recommendation is to monitor and regulate the sources of nutrient inputs into the reservoir, such as agricultural runoff, sewage, and industrial effluents, to reduce the risk of eutrophication and algal blooms. Another possible policy recommendation is to implement adaptive management strategies that consider the seasonal and spatial variability of Chl-a concentrations and their impacts on water quality and availability.