Application of Reverse Microdialysis in Neuropharmacological Studies

Rebuttal from Peter M. Lalley, Paul M. Pilowsky, Hubert V. Forster and Edward J. Zuperku

“…With reverse microdialysis, the drug is continuously released and targets a large volume due to diffusion in a complex interstitial space, which is not localized to a specific target (Höcht et al . ), e.g. only neurokinin‐1 receptor‐containing VRC neurons.…”

“…Microinjection studies rely on coordinates and functional properties of the preBötC (mixture of ventral respiratory column (VRC) I-and E-neurons) in addition to rapid onset tachypnoeic responses (Mustapic et al 2010) to D,L-homocysteic acid (DLH) microinjections (Schwarzacher et al 1995;Monnier et al 2003;Krolo et al 2005). With reverse microdialysis, the drug is continuously released and targets a large volume due to diffusion in a complex interstitial space, which is not localized to a specific target (Höcht et al 2013), e.g. only neurokinin-1 receptor-containing VRC neurons.…”