Application of ribotyping and IS200 fingerprinting to 
distinguish the five Salmonella serotype O6,7[ratio ]c[ratio ]1,5 groups: 
Choleraesuis sensu stricto, Choleraesuis var. Kunzendorf, 
Choleraesuis var. Decatur, Paratyphi C, and Typhisuis

Uzzau, Sergio; Hovi, Marianne; Stocker, B. A. D.

doi:10.1017/s0950268899002654

Cited by 22 publications

(23 citation statements)

References 18 publications

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“…In conclusion, Choleraesuis var. Kunzendorf appears to be monophyletic and distributed world-wide, whereas Choleraesuis non-Kunzendorf strains are mostly from South East Asia and as with the Indonesian isolates of Typhi, they show a certain degree of polymorphism by ribotype analysis [38].…”

Section: Taxonomy and Population Geneticsmentioning

confidence: 99%

“…This group of serotypes includes the swine-adapted Choleraesuis, the swine-restricted serotype Typhisuis, and the human-restricted Paratyphi C. Typhisuis has been reported to be polyphyletic by MLEE analysis, with clone Ts3 genotypically related to Decatur (also with serotype O: 6,7 : c : 1,5) and very different from Ts1 and Ts2 [24,37]. However, in a more recent study based on ribotyping, IS200 fingerprinting, and biochemical analysis, Ts3 was reclassified as Decatur [38]. Thus, Typhisuis is strongly homogeneous and supports the argument that HR serotypes are less diverse than those that are pathogenic for a wide variety of animal hosts.…”

Section: Taxonomy and Population Geneticsmentioning

confidence: 99%

“…Thus, Typhisuis is strongly homogeneous and supports the argument that HR serotypes are less diverse than those that are pathogenic for a wide variety of animal hosts. Furthermore, Typhisuis appeared to be allied to Choleraesuis and to certain clones of Paratyphi C [37,38]. It has been suggested that these serotypes evolved from a common ancestor that was already capable of systemic infection and adapted to swine and that the acquisition of the Vi antigen by Paratyphi C allowed this serotype to adapt to humans [24].…”

Section: Taxonomy and Population Geneticsmentioning

confidence: 99%

“…Uzzau and colleagues observed that Choleraesuis and its Kunzendorf variant had related but distinct ribotypes [38]. Application of ribotyping to Choleraesuis showed that Cs1 electrotype consists of Choleraesuis var.…”

Section: Taxonomy and Population Geneticsmentioning

confidence: 99%

“…Typhisuis is antigenically almost identical and genetically related to Choleraesuis [38,95]. Although these serotypes also share the same natural host, they differ significantly in several phenotypic characters and in the clinical aspects of the disease.…”

Section: Typhisuismentioning

confidence: 99%

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Host adapted serotypes of Salmonella enterica

et al. 2000

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Salmonella constitutes a genus of zoonotic bacteria of worldwide economic and health importance. The current view of salmonella taxonomy assigns the members of this genus to two species: S. enterica and S. bongori. S. enterica itself is divided into six subspecies, enterica, salamae, arizonae, diarizonae, indica, and houtenae, also known as subspecies I, II, IIIa, IIIb, IV, and VI, respectively [1]. Members of Salmonella enterica subspecies enterica are mainly associated with warm-blooded vertebrates and are usually transmitted by ingestion of food or water contaminated by infected faeces. The pathogenicity of most of the distinct serotypes remains undefined, and even within the most common serotypes, many questions remain to be answered regarding the interactions between the organism and the infected host.Salmonellosis manifests itself in three major forms: enteritis, septicaemia, and abortion, each of which may be present singly or in combination, depending on both the serotype and the host involved. Although currently over 2300 serovars of Salmonella are recognized, only about 50 serotypes are isolated in any significant numbers as human or animal pathogens [2, 3] and they all belong to subspecies enterica. Of these, most cause acute gastroenteritis characterized by a short incubation period and a severe systemic disease in man or animals, characterized by septicaemia, fever and/or abortion, and such serotypes are often associated with one or few host species [4–6].It is the intention of this review to present a summary of current knowledge of these host-adapted serotypes of S. enterica. The taxonomic relationships between the serotypes will be discussed together with a comparison of the pathology and pathogenesis of the disease that they cause in their natural host(s). Since much of our knowledge on salmonellosis is based on the results of work on Typhimurium, this serotype will often be used as the baseline in discussion. It is hoped that an appreciation of the differences that exist in the way these serotypes interact with the host will lead to a greater understanding of the complex host–parasite relationship that characterizes salmonella infections.

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Section: Taxonomy and Population Geneticsmentioning

confidence: 99%

Section: Taxonomy and Population Geneticsmentioning

confidence: 99%

Section: Taxonomy and Population Geneticsmentioning

confidence: 99%

Section: Taxonomy and Population Geneticsmentioning

confidence: 99%

Section: Typhisuismentioning

confidence: 99%

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Host adapted serotypes of Salmonella enterica

et al. 2000

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Two five-plex PCRs methods for identification of common Salmonella spp. serotypes

2010

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In Tunisia, Salmonella is the most common bacterial agent responsible for childhood diarrhoea. Currently, isolation of the bacterium by microbiological and biochemical methods and confirmation of the serotype by serological method remain as the "gold standard". This study aimed to differentiate among the most common serotypes of Salmonella spp. via two rapid five-plex PCRs assay (MPCR) to evaluate the molecular serotyping method compared with the gold standard serotyping technique. The two five-plex PCRs assays were designed for the simultaneous detection of six genetic loci from Salmonella enterica serovar Typhimurium and four from S. enterica serovar Typhi. Sixty-one Tunisian strains (46 collected from patients and 15 from food) were isolated during the period 2002-2007. The STM and STY primers were able to discriminate all tested Salmonella serotypes that represent the most common clinical and food strains of S. enterica subsp. enterica in our laboratory. All strains belonged to 19 different serotypes: 15 serotypes gave unique amplification patterns compared each other and the other 4 serotypes were grouped into two pairs that gave the same molecular profile. We resolved this problem through the addition of a monoplex PCR. Salmonella typhimurium ATCC 14028 consistently produced the same molecular profile as S. typhimurium laboratory isolates. Interestingly, seven strains of Anatum serovar produced two different PCR profiles with these primers: five strains had the same amplification pattern STM 2,4,5 and STY 2; however, two strains had another molecular profile STM 2,3,5 and STY 2; so the reproducibility of this method was reduced to 93%. The MPCR system is a rapid, specific, and cost-effective molecular method that gas been proved to have efficient discrimination in serotyping of the most common isolates of S. enterica subsp. enterica.

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