Application of salicylic acid induces antioxidant defense responses in the phloem of Picea abies and inhibits colonization by Ips typographus

Krajnc, Andreja Urbanek; Kristl, Janja; Ivančič, Anton

doi:10.1016/j.foreco.2010.10.027

Cited by 24 publications

(8 citation statements)

References 69 publications

(126 reference statements)

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“…Interestingly, treatment of susceptible tobacco with SA causes a profound reduction in TMV accumulation. This effect occurs with no macroscopically visible cell death, and associated with ROS mediated signaling (Chivasa et al 1997;Urbanek Krajnc et al 2011) . Enhanced levels of SA and decreased levels of JA were observed in all transgenic plant lines that overexpress MP, which suggests that at least this viral protein is sufficient to modulate the stress and defense responses in tobacco plants.…”

Section: Discussionmentioning

confidence: 95%

Transgenic Expression ofTobacco mosaic virusCapsid and Movement Proteins Modulate Plant Basal Defense and Biotic Stress Responses inNicotiana tabacum

Conti¹,

Rodríguez²,

Manacorda³

et al. 2012

MPMI

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Plant viruses cause metabolic and physiological changes associated with symptomatic disease phenotypes. Symptoms involve direct and indirect effects, which result in disruption of host physiology. We used transgenic tobacco expressing a variant of Tobacco mosaic virus (TMV) coat protein (CP(T42W)) or movement protein (MP), and a hybrid line (MP×CP(T42W)) that coexpresses both proteins, to study the plant response to individual viral proteins. Findings employing microarray analysis of MP×CP(T42W) plants and silenced mp×cp(T42W)* controls revealed that altered transcripts were mostly downregulated, suggesting a persistent shut-off due to MP×CP(T42W) expression. Next, we showed that MP triggered reactive oxygen species (ROS) accumulation, reduction of total ascorbate, and expression of ROS scavenging genes. These effects were enhanced when both proteins were coexpressed. MP and MP×CP(T42W) plants showed increased levels of salicylic acid (SA) and SA-responsive gene expression. Furthermore, these effects were partially reproduced in Nicotiana benthamiana when GMP1 transcript was silenced. CP(T42W) seems to be playing a negative role in the defense response by reducing the expression of PR-1 and RDR-1. MP and MP×CP(T42W) transgenic expression promoted a recovery-like phenotype in TMV RNA infections and enhanced susceptibility to Pseudomonas syringae and Sclerotinia sclerotiorum. The individual effects of viral proteins may reflect the ability of a virus to balance its own virulence.

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Section: Discussionmentioning

confidence: 95%

Transgenic Expression ofTobacco mosaic virusCapsid and Movement Proteins Modulate Plant Basal Defense and Biotic Stress Responses inNicotiana tabacum

Conti¹,

Rodríguez²,

Manacorda³

et al. 2012

MPMI

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“…SA potentiates the oxidative burst and facilitates programmed cell death in several plant–pathogen interactions (Durrant & Dong, ). However, enzymatic and non‐enzymatic antioxidants are also activated during the development of SA‐induced SAR (Fodor et al , ; Urbanek Krajnc et al , ) but down‐regulated in a transgenic SA‐deficient tobacco that fails to develop SAR following virus infection (Király et al , ).…”

Section: Discussionmentioning

confidence: 99%

Seed treatment with salicylic acid invokes defence mechanism ofHelianthus annuusagainstOrobanche cumana

Yang

Ali

et al. 2016

Annals of Applied Biology

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The root holoparasitic angiosperm sunflower broomrape (Orobanche cumana) specifically affects sunflower (Helianthus annuus) growth and causes severe damage all over the world. This investigation was designed to examine the protective effects of salicylic acid (SA) treatment to the seeds of an O. cumana-susceptible cultivar of sunflower (TK0409). Sunflower seeds were pretreated with different concentrations (0, 0.5 and 1 mM) of SA and inoculated with O. cumana for 4 weeks. O. cumana infection resulted in reduction in plant biomass, endogenous SA level, and the expression of SA-related genes including pal, chs and NPR1. By contrast, O. cumana infection enhanced the production of reactive oxygen species, activities of antioxidant enzymes as well as contents of phenolics and lignin. Seed treatment with 1 mM SA increased sunflower biomass in terms of plant height, fresh weight and dry weight by 10%, 13% and 26%, respectively, via reducing the number and biomass of established O. cumana. The increase of hydrogen peroxide contents by 14% in the 1 mM SA treated sunflower plants appeared to be because of the inhibition of ascorbate peroxidase and catalase by exogenous SA. The enhanced expression of pathogenesis-related genes (PR3 and PR12, encoding chitinase and defensin, respectively) after 4 weeks of inoculation indicated that systemic acquired resistance was induced in the SA treated sunflower in which the level of endogenous SA was also elevated in a dose-dependent manner. The increased expression of a hypersensitive-responsive (HR) gene hsr indicated that the resistance of sunflowers might be associated with a hypersensitive reaction which was activated by exogenous SA treatment.(H 2 O 2 ) in O. cumana-infected sunflowers promote an oxidative burst during the interaction of sunflower and O. cumana . Rapid production of ROS is seen as an indicator of successful recognition of infection by plants, leading to the activation of the plants' defence mechanism (Torres et al., 2006;Torres, 2010). Accumulation of H 2 O 2 in peas infected by Orobanche crenata was observed by using both biochemical and cytochemical techniques (Pérez-de-Luque et al., 2006;Mabrouk et al., 2007). However, Mor et al. (2008) found that ROS detected at the interaction site on Arabidopsis thaliana infected by Orobanche aegyptiaca probably originated from the parasite, which was important for 408 Ann Appl Biol 169 (2016) 408-422

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“…Miller et al ( 2005 ) have demonstrated that feeding by the white pine weevil ( Pissodes strobi ) results in the induction of genes leading to JA in Sitka spruce ( Picea sitchensis ) by RNA-blot experiments. Urbanek-Krajnc et al ( 2011 ) showed that application of salicylate induces antioxidant defense responses in the phloem of P. abies and inhibits colonization by I. typographus .…”

Section: Introductionmentioning

confidence: 99%

Induction of isoprenyl diphosphate synthases, plant hormones and defense signalling genes correlates with traumatic resin duct formation in Norway spruce (Picea abies)

et al. 2011

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Norway spruce (Picea abies) defends itself against herbivores and pathogens by formation of traumatic resin ducts filled with terpenoid-based oleoresin. An important group of enzymes in terpenoid biosynthesis are the short-chain isoprenyl diphosphate synthases which produce geranyl diphosphate (C10), farnesyl diphosphate (C15), and geranylgeranyl diphosphate (C20) as precursors of monoterpenes, sesquiterpenes, and diterpene resin acids, respectively. After treatment with methyl jasmonate (MJ) we investigated the expression of all isoprenyl diphosphate synthase genes characterized to date from Norway spruce and correlated this with formation of traumatic resin ducts and terpene accumulation. Formation of traumatic resin ducts correlated with higher amounts of monoterpenes, sesquiterpenes and diterpene resin acids and an upregulation of isoprenyl diphosphate synthase genes producing geranyl diphosphate or geranylgeranyl diphosphate. Among defense hormones, jasmonate and jasmonate-isoleucine conjugate accumulated to higher levels in trees with extensive traumatic resin duct formation, whereas salicylate did not. Jasmonate and ethylene are likely to both be involved in formation of traumatic resin ducts based on elevated transcripts of genes encoding lipoxygenase and 1-aminocyclopropane-1-carboxylic acid oxidase associated with resin duct formation. Other genes involved in defense signalling in other systems, mitogen-activated protein kinase3 and nonexpressor of pathogenesis-related gene1, were also associated with traumatic resin duct formation. These responses were detected not only at the site of MJ treatment, but also systemically up to 60 cm above the site of treatment on the trunk.Electronic supplementary materialThe online version of this article (doi:10.1007/s11103-011-9832-7) contains supplementary material, which is available to authorized users.

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Application of salicylic acid induces antioxidant defense responses in the phloem of Picea abies and inhibits colonization by Ips typographus

Cited by 24 publications

References 69 publications

Transgenic Expression ofTobacco mosaic virusCapsid and Movement Proteins Modulate Plant Basal Defense and Biotic Stress Responses inNicotiana tabacum

Transgenic Expression ofTobacco mosaic virusCapsid and Movement Proteins Modulate Plant Basal Defense and Biotic Stress Responses inNicotiana tabacum

Seed treatment with salicylic acid invokes defence mechanism ofHelianthus annuusagainstOrobanche cumana

Induction of isoprenyl diphosphate synthases, plant hormones and defense signalling genes correlates with traumatic resin duct formation in Norway spruce (Picea abies)

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