Application of Scale-Up Criterion of Constant Oxygen Mass Transfer Coefficient (kLa) for Production of Itaconic Acid in a 50 L Pilot-Scale Fermentor by Fungal Cells of Aspergillus terreus

Shin, Woo-Shik

doi:10.4014/jmb.1307.07084

Cited by 33 publications

(29 citation statements)

References 28 publications

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“…Petruccioli et al reported the maximum IA production of 19.8 g/L in 3 L stirred tank bioreactor using corn starch. In a previous study, Shin et al performed fermentation in 5 L stirred tank bioreactor and obtained the highest IA production under the operating condition of 200 rpm and 1.5 vvm. The IA production obtained in bioreactor was lower than that from flask level in this study.…”

Section: Discussioncontrasting

confidence: 51%

Optimization and scale up of itaconic acid production from potato starch waste in stirred tank bioreactor

Bafana

Sivanesan

Pandey

2019

Biotechnology Progress

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Present study used Aspergillus terreus strain C1 isolated from mangrove soil for itaconic acid (IA) production from potato starch waste. Fermentation parameters were optimized by classical one factor approach and statistical experimental designs, such as Plackett-Burman and response surface designs. Anionic deionization of potato waste was found to be a very effective, economic, and easy way of improving IA production. The increase in IA production by deionization was found to correlate with removal of phosphate. In our knowledge, this is the first report on application of deionization of potato waste to enhance IA production. Other parameters like inoculum development conditions, pH, presence of peptone and certain salts in the medium also significantly affected IA production. IA production by strain C1 increased 143-fold during optimization when compared with the starting condition. The optimized IA level (35.75 g/L) was very close to the maximum production predicted by RSM (38.88 g/L). Bench scale production of IA was further optimized in 3-L stirred tank reactor by varying parameters like agitation and aeration rate. The maximum IA production of 29.69 g/L was obtained under the agitation speed of 200 rpm and aeration rate of 0.25 vvm. To the best of our knowledge, it is the first report on IA production from potato starch waste at bioreactor level.

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Section: Discussioncontrasting

confidence: 51%

Optimization and scale up of itaconic acid production from potato starch waste in stirred tank bioreactor

Bafana

Sivanesan

Pandey

2019

Biotechnology Progress

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“…It is also known that the microbial physiology of filamentous fungal cells is significantly influenced by the DO concentration in suspended cultures, and it has been suggested that the critical DO concentration for fungal cells in culture should be greater than around 20% of the saturation DO value [45,46]. In this study, no effort was made to maintain the DO above 20% in order to investigate the effect of DO on enzyme production by the fungus T. reesei MUM 97.53.…”

Section: Influence Of Aeration and Agitation Rates In The Enzymatic Pmentioning

confidence: 98%

Production of Biomass-Degrading Enzymes by Trichoderma reesei Using Liquid Hot Water-Pretreated Corncob in Different Conditions of Oxygen Transfer

et al. 2019

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Enzymatic hydrolysis accounts for 20% of the total cost in the conversion process of lignocellulosic biomass into bioethanol. Therefore, production of biomass-degrading enzymes by using lignocellulosic residue as a fermentation substrate may be an alternative to decrease the production costs. In this study, corncob (CC) has been pretreated by liquid hot water (LHW) at 200°C for 30 min and used as inducer source for production of biomass-degrading enzymes by Trichoderma reesei MUM 97.53. The pretreatment was used to increase the cellulose content and the accessibility to lignocellulosic material. Although the filamentous fungus secreted a broad range of cellulolytic and hemicellulolytic enzymes when grown on untreated CC, higher enzyme productions were obtained when cultured on LHW-pretreated CC in a 2-L stirred tank bioreactor (STB). Besides, the effects of aeration (2 and 4 vvm) and agitation (150 and 250 rpm) rates on enzyme production were studied by submerged fermentation in a batch STB and correlated with the volumetric oxygen transfer coefficient (k L a). Maximal cellulase, xylanase, and βxylosidase productions were found at 150 rpm and 4 vvm, while the highest β-glucosidase levels were obtained at 150 rpm and 2 vvm, that corresponded to k L a values of 32.50 h −1 and 16.41 h −1 , respectively. At higher agitation, a lower enzymatic production was observed probably due to the high shear stress in the fungal hyphae.

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“…When the impeller tip speed was 94.2 cm s −1 at a fixed aeration rate of 0.5 vvm, IA production was 3.6 and 1.6 times higher than those in the impeller speed of 62.8 and 125.7 cm s −1 , respectively. Shin et al [81] applied the scale-up criterion of constant oxygen mass transfer coefficient (k L a) for production of IA from glucose in 5 L and 50 L scale fermenters by A. terreus cells. The highest IA production (0.71 g g −1 glucose) was obtained under operating conditions of 200 rpm and 1.5 vvm in a 5 L fermenter, and 180 rpm and 0.5 vvm in a 50 L fermenter.…”

Section: Production Of Itaconic Acid By Aspergillus Terreusmentioning

confidence: 99%

Emerging biotechnologies for production of itaconic acid and its applications as a platform chemical

Saha¹

2017

Journal of Industrial Microbiology and Biotechnology

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Recently, itaconic acid (IA), an unsaturated C5-dicarboxylic acid, has attracted much attention as a biobased building block chemical. It is produced industrially (>80 g L) from glucose by fermentation with Aspergillus terreus. The titer is low compared with citric acid production (>200 g L). This review summarizes the latest progress on enhancing the yield and productivity of IA production. IA biosynthesis involves the decarboxylation of the TCA cycle intermediate cis-aconitate through the action of cis-aconitate decarboxylase (CAD) enzyme encoded by the CadA gene in A. terreus. A number of recombinant microorganisms have been developed in an effort to overproduce it. IA is used as a monomer for production of superabsorbent polymer, resins, plastics, paints, and synthetic fibers. Its applications as a platform chemical are highlighted. It has a strong potential to replace petroleum-based methylacrylic acid in industry which will create a huge market for IA.

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Application of Scale-Up Criterion of Constant Oxygen Mass Transfer Coefficient (kLa) for Production of Itaconic Acid in a 50 L Pilot-Scale Fermentor by Fungal Cells of Aspergillus terreus

Cited by 33 publications

References 28 publications

Optimization and scale up of itaconic acid production from potato starch waste in stirred tank bioreactor

Optimization and scale up of itaconic acid production from potato starch waste in stirred tank bioreactor

Production of Biomass-Degrading Enzymes by Trichoderma reesei Using Liquid Hot Water-Pretreated Corncob in Different Conditions of Oxygen Transfer

Emerging biotechnologies for production of itaconic acid and its applications as a platform chemical

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