Application of scanning ion conductance microscope in cell characterizations

Lang, Jinxin; Li, Yabei; Yang, Yo-Lun; Jiao, Yangbohan; Wang, Meng; Zhuang, Jian; Li, Fēi

doi:10.1360/n032018-00266

Cited by 1 publication

(1 citation statement)

References 59 publications

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“…After detecting the imaging point information, the probe retracts to a preset height, moves forward to the next imaging point, and then repeats the downward detection to complete the scan. Throughout the scanning process, the ion current fluctuates within a very small range, approximating a straight line, known as the “ion current change curve” [ 13 ], as shown in Figure 1 c.…”

Section: Sicm Imaging Temporal Resolution and Noise Analysismentioning

confidence: 99%

Research on an SICM Scanning Image Resolution Enhancement Algorithm

Quan,

Xu,

Liao

et al. 2024

Sensors

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Scanning ion conductance microscopy (SICM) enables the non-invasive three-dimensional imaging of live cells and other structures in physiological environments. However, when imaging complex samples, SICM faces challenges such as having a low temporal resolution during slow scanning and a reduced signal-to-noise ratio during fast scanning, making it difficult to simultaneously improve both temporal and spatial resolution. To address these issues, this paper proposes an algorithm for enhancing image resolution under high-speed scanning. Firstly, scanning images are preprocessed using a median filtering algorithm to remove the salt-and-pepper noise generated during high-speed scanning. Next, the Canny edge detection algorithm is employed to extract the edges of the image targets. To avoid blurring the edges, the new edge-directed interpolation (NEDI) algorithm is then used to fill the edges, while non-edge areas are filled using bilinear interpolation, thereby enhancing the image resolution. Finally, the peak signal-to-noise ratio (PSNR) and structural similarity index (SSIM) are used to analyze the imaging of articular chondrocytes. The results show that under a scanning speed of 480 nm/ms, the proposed algorithm improves the temporal resolution of imaging by 60% compared to traditional 2× resolution imaging, increases the peak signal-to-noise ratio of the scanning images by 7 dB, and achieves a structural similarity of 0.97. Therefore, the proposed algorithm effectively removes noise during high-speed scanning and improves the SICM scanning imaging resolution, thereby avoiding the reduction in temporal resolution when scanning larger resolution samples and effectively enhancing the performance of SICM scanning imaging.

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Section: Sicm Imaging Temporal Resolution and Noise Analysismentioning

confidence: 99%