Application of <scp>DNA</scp> markers for discrimination between Japanese and Australian Wagyu beef

Kawaguchi, Fuki; Kitamura, Yuto; Nakajima, Ryuji; Takahashi, Masayoshi; Goto, Hiroaki; Washida, Yuichi; Yamamoto, Yoshikazu; Sasazaki, Shinji; Mannen, Hideyuki

doi:10.1111/asj.12938

Cited by 5 publications

(3 citation statements)

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“…Western blot analysis. Western blot analysis was performed as previously described 11,24,32 . Briefly, cells or homogenized liver samples were lysed with lysis buffer (62.5 mM tris at pH 6.8, 3% SDS, protease inhibitor [Roche, 05056489001]).…”

Section: Methodsmentioning

confidence: 99%

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MCPIP1 reduces HBV-RNA by targeting its epsilon structure

Que

Fukano

et al. 2020

Sci Rep

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Hepatitis B virus (HBV) is the major causative factor of chronic viral hepatitis, liver cirrhosis, and hepatocellular carcinoma. We previously demonstrated that a proinflammatory cytokine IL-1β reduced the level of HBV RNA. However, the mechanism underlying IL-1β-mediated viral RNA reduction remains incompletely understood. In this study, we report that immune regulator Monocyte chemotactic protein-1-induced protein 1 (MCPIP1) can reduce HBV RNA in hepatocytes. MCPIP1 expression level was higher in the liver tissue of HBV-infected patients and mice. Overexpression of MCPIP1 decreased HBV RNA, whereas ablating MCPIP1 in vitro enhanced HBV production. The domains responsible for RNase activity or oligomerization, were required for MCPIP1-mediated viral RNA reduction. The epsilon structure of HBV RNA was important for its antiviral activity and cleaved by MCPIP1 in the cell-free system. Lastly, knocking out MCPIP1 attenuated the anti-HBV effect of IL-1β, suggesting that MCPIP1 is required for IL-1β-mediated HBV RNA reduction. Overall, these results suggest that MCPIP1 may be involved in the antiviral effect downstream of IL-1β.

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Section: Methodsmentioning

confidence: 99%

“…CRISPR/Cas9-mediated gene targeting. MCPIP1 knockout cells were established as described previously 32 . The MCPIP1 CRISPR plasmids (Santa Cruz, sc-401790-NIC, sc-40190-NIC-2) were transfected into Hep38.7-Tet cells.…”

Section: Methodsmentioning

confidence: 99%

MCPIP1 reduces HBV-RNA by targeting its epsilon structure

Que

Fukano

et al. 2020

Sci Rep

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“…As a rule, these methods are directed towards revealing a relationship between the analyzed samples and the indicators that characterize one or another region. These indicators include the quantity or ratio of micro-, macro-elements, heavy metals, radioelements, stable isotopes, comparison of DNA (Lo and Shaw, 2018; Kawaguchi et al, 2018), microflora of a sample (Nguen et al, 2008) and a reference. The differences in the content of 90 Sr in cheeses produced in different European countries were found.…”

Section: Introductionmentioning

confidence: 99%

Study on the meat isotopic composition for origin identification

Чернуха

Yurchak

Кузьмина

2018

Potr. S. J. F. Sci.

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Russian consumer and governmental authorities are equally concerned to know where food products come from. This requires more accurate and specialized methods for the evaluation of geographical location. The following methods are used: chemometrics, histological and histochemical, genomic and proteomic, microbiological, immunochemical and mass spectrometric. Method of stable isotope analysis is becoming increasingly promising nowadays for the identification of meat and meat products' place of origin. The isotope ratios of the four elements -carbon, nitrogen, oxygen and hydrogen, are mainly determined. The method is successfully used to identify a country of origin of wines, juices and water. The aim of the research was to study the stable isotope ratios for pork and beef samples purchased in Moscow supermarkets (Russian Federation). The country of production of meat samples was determined according to specifications and/or labels. The geography of countries of meat samples origin includes Europe, both America continents and Australia. Databases collected by the All-Russian Scientific Research Institute of the Brewing, Non-Alcoholic and Wine Industrywere used for the analysis and interpretation of the results. Values of 13 С/ 12 С, δ 13 С, 18 / 16 , δ 18 , 2 / 1 , δ 2 for 30 pork and beef samples from 13 countries were obtained. Differences in stable isotope ratios were found depending on place of origin. The data correlated with the oxygen isotope characteristics for wine, which were in the range from 2.5 to 4.5 ppm. According to the 13 С/ 12 С, δ 13 С results, the assumption was made about a false indication of the region for the beef sample. Despite the fact that beef was labeled as a product of Lithuania, the region of origin was most probably defined as Germany. The studies carried out showed the possibility to identify the region of raw meat origin by the stable isotope ratio.

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