Application of <scp>MALDI</scp>‐<scp>TOF</scp> mass spectrometry for in vitro identification of wood decay polypores

Pristaš, Peter; Kvasnová, Simona; Gáperová, Svetlana; Gašparcová, T.; Gáper, Ján

doi:10.1111/efp.12352

Cited by 11 publications

(7 citation statements)

References 8 publications

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“…In the forests of Slovakia, G. applanatum was also found growing predominantly on Fagus sylvatica L. (38% off all findings). However, compared to urban areas, it was recorded only on 11 species of host trees in forests (Gašparcová et al, 2017). Although more records of this species are known from the forest environment (Gašparcová et al, 2017), G. applanatum is the second most widespread species in urban areas of Slovakia (Fig.…”

Section: Resultsmentioning

confidence: 99%

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Host preferences of wood-decaying fungi of the genus Ganoderma in the urban areas of Slovakia

et al. 2018

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Ganoderma (Basidiomycota, Polyporales) is a cosmopolitan genus with the greatest diversity in the tropics. It causes white rot of a wide range of woody plants all over the world. In Europe, 7 species of the genus Ganoderma grow: Ganoderma adspersum, G. applanatum, G. carnosum, G. lucidum, G. pfeifferi, G. resinaceum and G. valesiacum. All of them also occur in Slovakia. The aim of the present study was to identify the presence of Ganoderma species in the urban areas of Slovakia and to find out their host preferences and topology of the basidiocarps on the colonised trees. A total 263 findings of Ganoderma obtained by own field research and processing of records from herbarium items located in natural museums in Bratislava (Slovakia) and Prague (Czech Republic) were analysed to detect their ecological characteristics. The occurrence of all 7 Ganoderma species was recorded in the urban areas of Slovakia. The most common species is G. adspersum (39% of all records) with the widest range of host woody plants (24 species). G. applanatum is second most widespread species in the urban areas of Slovakia growing mostly on a decomposing wood of unknown trees (31% of all findings) and also on beeches (9% of all findings). G. resinaceum is third most widespread in the urban areas and it grew mainly on Quercus. G. lucidum was most often found on unknown trees (65% of all findings) and also on oaks (16% of all findings). The rarest Ganoderma species are G. valesiacum (only 1 record on Larix sp.), G. pfeifferi (4 records, mainly on Fagus sylvatica) and G. carnosum (4% of all records, mainly on a decomposing wood of unknown trees).

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Section: Resultsmentioning

confidence: 99%

“…It can also colonise living trees, mostly the bases of the trunks (Sokół, 2000;Bernicchia, 2005;Ryvarden, Melo, 2014). In the forests of Slovakia, it was found to grow mainly on trunks and less often on stumps (Gašparcová et al, 2017).…”

Section: Resultsmentioning

confidence: 99%

Host preferences of wood-decaying fungi of the genus Ganoderma in the urban areas of Slovakia

et al. 2018

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“…Species-specific VOCs have already been defined for a few polypore species (Marshall 1970;Cowan 1973;McAfee & Taylor 1999;Rapior et al 2000;Rosecke et al 2000;Ziegenbein et al 2010;Konuma et al 2015). More generally, this confirms that direct mass spectrometry allows for a reliable species identification of wood decaying polypores, including a discrimination between F. fomentarius and Fomes inzengae (Pristas et al 2017).…”

Section: Volatile Organic Compoundsmentioning

confidence: 53%

How to resolve cryptic species of polypores: an example in Fomes

et al. 2019

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Species that cannot be easily distinguished based on morphology, but which form distinct phylogenetic lineages based on molecular markers, are often referred to as cryptic species. They have been proposed in a number of fungal genera, including the basidiomycete genus Fomes. The main aim of this work was to test new methods for species delimitation in cryptic lineages of polypores, and to define useful characters for species identification. A detailed examination of a number of different Fomes strains that had been collected and isolated from different habitats in Italy and Austria confirmed the presence of distinct lineages in the Fomes fomentarius clade. Our zero hypothesis was that the Mediterranean strains growing on Quercus represent a species which can be delimited based on morphological and physiological characters when they are evaluated in statistically relevant numbers. This hypothesis was tested based on phylogenetic analysis of the rDNA ITS region, morphological characters of basidiomes and pure cultures, growth rates and optimum growth temperature experiments, mycelial confrontation tests, enzyme activity tests and volatile organic compound (VOC) production. The Mediterranean lineage can unambiguously be delimited from F. fomentarius. A syntype of an obscure and previously synonymized name, Polyporus inzengae, represents the Mediterranean lineage that we recognize as Fomes inzengae, a distinct species. The rDNA ITS region is useful for delimitation of Fomes species. Moreover, also a variety of morphological characters including hymenophore pore size, basidiospore size, and diameter of skeletal hyphae are useful delimiting characters. The ecology is also very important, because the plant host appears to be a central factor driving speciation. Physiological characters turned also out to be species-specific, e.g. daily mycelial growth rates or the temperature range of pure cultures. The production of VOCs can be considered as a very promising tool for fast and reliable species delimitation in the future.

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“…(Reddel et al 2019 ). Moreover, this technique was also found effective in the identification and discrimination of eight polypore species of genre Fomitopsis, Rhodofomes, Fomes , and Ganoderma in, Czech Republic (Pristaš et al 2017 ). It has also successfully aided in the identification of two closely related Vibrio sp., V. furnissii and V. fluvialis, both representing human pathogen associated with the consumption of contaminated seafood and contaminated water (Schirmeister et al 2014 ).…”

Section: Microbial Ecologymentioning

confidence: 99%

MALDI-TOF MS: application in diagnosis, dereplication, biomolecule profiling and microbial ecology

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Nisa

2022

Proc.Indian Natl. Sci. Acad.

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Application of MALDI‐TOF mass spectrometry for in vitro identification of wood decay polypores

Cited by 11 publications

References 8 publications

Host preferences of wood-decaying fungi of the genus Ganoderma in the urban areas of Slovakia

Host preferences of wood-decaying fungi of the genus Ganoderma in the urban areas of Slovakia

How to resolve cryptic species of polypores: an example in Fomes

MALDI-TOF MS: application in diagnosis, dereplication, biomolecule profiling and microbial ecology

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