Application of self-assembly techniques in the design of biocompatible protein microarray surfaces

Abstract

This review focuses on the application of novel technologies for generating biocompatible surfaces for high-throughput screening (HTS) of proteins. Various methods of coupling and spotting proteins on self-assembled monolayer (SAM) surfaces will be described along with the protein chip challenges pertaining to spot homogeneity, morphology, biocompatibility and reproducibility.

Surface Plasmon Resonance Imaging of Biomolecular Interactions on a Grating-Based Sensor Array

“…44,45 Similar self-assembly techniques have also been used to construct protein microarrays. 46 We chose MUA, MUL, and MUN to construct modified gold surfaces. MUA is a convenient choice for covalent coupling to proteins since NHS activation can be used to create a surface that will readily react with pendant primary amines.…”

Development of a fluorescent and colorimetric detection methods-based protein microarray for serodiagnosis of TORCH infections

“…However, some intrinsic shortcomings in fabrication technologies potentially affect developments and applications of protein microarray because it is difficult to maintain active forms of captured proteins on a solid surface in such a way that their three-dimensional structures, functionalities and binding sites are retained intact and the biochemical diversity and the sheer number of proteins are such that an equivalent analysis is much more complex and thus difficult to accomplish Schaeferling et al, 2002). To minimize the disadvantages, various chemical surfaces on glass slides were tested with a number of coupling chemistries, such as amine (Duburcq et al, 2004a), aldehyde (Zammatteo et al, 2000), poly-l-lysine (Haab et al, 2001), epoxy , agarose (Afanassiev et al, 2000), polyacrylamide (Angenendt et al, 2002) and nitrocellulose (Qiu et al, 2004).…”

Synthesis of Thiol-Functionalized Ionic Liquids and Formation of Self-Assembled Monolayer on Gold Surfaces: Effects of Alkyl Group and Anion on the Surface Wettability

“…Controlling wettability of the solid surfaces by surface modification has intensively been studied due to many technologically important applications. [16][17][18] Among the surface modification methods, the formation of self-assembled monolayers (SAMs) proved to be a simple and practical technique for controlling wettability, [19][20][21][22][23][24][25] corrosion, [26][27][28] and (bio)adhesion [29][30][31][32][33][34] of solid surfaces. On the basis of SAMs, the wettability of solid surfaces could be controlled in various ways, i.e., environmental changes (solvents, [35][36][37] pH, 38 temperature, [39][40][41] and surface pressure 42 ) or external stimuli (i.e., light, 23,24 charge, 25 and oxidation-reduction 43 ).…”