Application of simplified Fuhrman grading system in clear‐cell renal cell carcinoma

Hong, Sung Kyu; Jeong, Chang Wook; Park, Ji H.; Kim, Hyung S.; Kwak, Cheol; Choe, Gheeyoung; Kim, Hyeon H.; Lee, Sangeun

doi:10.1111/j.1464-410x.2010.09561.x

Cited by 30 publications

(26 citation statements)

References 22 publications

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“…The study cohort consisted of 123 patients who underwent radical nephrectomy or partial nephrectomy for ccRCC between July and September 2012 at the Department of Urology, Peking University First Hospital (Beijing, China). General patient characteristics, including age, gender and tumor features, such as location, T stage and Fuhrman grade (7,8), were collected. The present study was approved by the institutional review board of Peking University First Hospital.…”

Section: Methodsmentioning

confidence: 99%

Association between FBP1 and hypoxia-related gene expression in clear cell renal cell carcinoma

Ning¹,

Teng²,

Gong³

et al. 2016

Oncology Letters

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Abstract. Fructose-1,6-bisphosphatase 1 (FBP1) is a rate-limiting enzyme in gluconeogenesis. Recently, the catalytic activity-independent function of FBP1, hypoxia-induced factor (HIF) repression in the nucleus, was identified. The aim of the present study was to investigate the association between FBP1 and hypoxia-related gene expression in clear cell renal cell carcinoma (ccRCC). The protein expression levels of FBP1, HIF-1α, HIF-2α, erythropoietin (EPO) and carbonic anhydrase IX (CA9) were assessed by immunohistochemical staining of ccRCC paraffin blocks from 123 patients using the tissue microarray technique. The expression level of FBP1 was then correlated with various clinicopathological factors, and the protein expression levels of HIF-1α, HIF-2α, EPO and CA9. Clinicopathological factors, including age, gender, T stage and Fuhrman grade, were not significantly different between patients with low and high FBP1 expression in ccRCC (P>0.05). FBP1 protein expression level was significantly correlated with the expression levels of HIF-1α (P=0.005) and EPO (P= 0.010), but not significantly correlated with the expression levels of HIF-2α (P=0.123) and CA9 (P=0.513) in ccRCC tissues. The current findings confirm the association between FBP1 and hypoxia-related gene expression, and may facilitate understanding of the mechanisms of ccRCC tumorigenesis.

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Section: Methodsmentioning

confidence: 99%

Association between FBP1 and hypoxia-related gene expression in clear cell renal cell carcinoma

Ning¹,

Teng²,

Gong³

et al. 2016

Oncology Letters

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“…The study was approved by the Ethics Committee of The Second Affiliated Hospital of Xi'an Jiaotong University. The tumors were graded according to the criteria of the Fuhrman grading system of malignant tumors, and the clinical tumor stage was classified according to the tumor, node, metastasis classification system (25,26). RCC specimens and corresponding normal healthy kidney tissues located as far as possible from the tumor were obtained.…”

Section: Methodsmentioning

confidence: 99%

TPX2 in human clear cell renal carcinoma: Expression, function and prognostic significance

et al. 2016

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Abstract. Targeting protein for Xenopus kinesin-like protein 2 (TPX2) is a microtubule-associated protein. TPX2 is considered to be an important gene in tumorigenesis; however, the particular function of TPX2 in the development of human renal cell carcinoma (RCC) is unknown. In the present study, the expression, function and prognostic significance of TPX2 in human RCC was analyzed. A total of 286 tissue samples from patients with RCC who had undergone nephrectomies were utilized. Subsequently, the expression of TPX2 protein was investigated using immunohistochemistry and western blotting, and TPX2 mRNA expression was examined using reverse transcription-quantitative polymerase chain reaction. To establish the effect of TPX2 on the proliferation and invasion of the RCC cells, TPX2 expression was increased by stable transfection with a TPX2 vector and TPX2 expression was decreased using small interfering RNA. Proliferation of the RCC cells was analyzed using a WST-1 assay and an animal xenograft model with BALB/c nude mice, whilst invasion of the RCC cells was examined using a Matrigel-coated invasion chamber. It was demonstrated that TPX2 expression was significantly higher in the RCC tissues compared with normal kidney tissues (P<0.05). Furthermore, TPX2 expression was associated with tumor size, histological grade and tumor stage (P<0.05), and was observed to markedly increase the proliferation and invasion of the RCC cells. It may be concluded that the expression of TPX2 is significantly upregulated in RCC tissue, subsequently increasing the proliferative and invasive ability of RCC cells. Therefore, the protein may serve as a therapeutic target and independent prognostic factor in the treatment of human RCC.

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“…The histological cell type of all specimen slices was determined by experienced pathologists and all samples were diagnosed as conventional CCRCC. The clinical tumor stages and characteristics were classified according to the tumor node metastasis (TNM) classification system (41), and the nuclear grade was evaluated according to the Fuhrman grading system of malignant tumors (42). RCC tissue samples and corresponding healthy kidney tissues located at a maximal distance from the tumor were collected immediately following surgical resection.…”

Section: Methodsmentioning

confidence: 99%

Forkhead box J1 expression is upregulated and correlated with prognosis in patients with clear cell renal cell carcinoma

et al. 2015

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Abstract. The forkhead box (FOX) family of transcription factors are considered to have a role in tumorigenesis. FOXJ1 is a member of the FOX family; however, its function in human renal cell carcinoma (RCC) has remained to be elucidated. Therefore, the present study evaluated the expression of FOXJ1 in human clear cell RCC and the effect of FOXJ1 on the proliferative ability of RCC cells. The RCC specimens analyzed in the present study were obtained from 286 patients with RCC who underwent nephrectomy. FOXJ1 mRNA expression levels were determined using reverse transcription-quantitative polymerase chain reaction, and FOXJ1 protein expression levels were determined using immunohistochemistry and western blot analysis. To determine the effect of FOXJ1 on the proliferative ability of RCC cells, the expression of FOXJ1 was decreased using small interfering (si)RNA, and a FOXJ1 vector was stably transfected into RCC cell lines. The proliferative ability of RCC cells was then examined using a WST-1 assay and xenograft experiments with BALB/c nude mice, where the association between FOXJ1 expression and patient survival was determined using Kaplan-Meier analysis. FOXJ1 expression was significantly higher in RCC tissues compared with that of healthy renal tissues. Furthermore, FOXJ1 expression was associated with tumor stage, histologic grade and size. In addition, FOXJ1 significantly enhanced the proliferation of RCC cells in vitro and in vivo. The present study identified that FOXJ1 expression was upregulated in RCC and enhanced the proliferative ability of RCC cells. Therefore, FOXJ1 may serve as an independent prognostic marker and a therapeutic target for the treatment of patients with RCC.

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Application of simplified Fuhrman grading system in clear‐cell renal cell carcinoma

Cited by 30 publications

References 22 publications

Association between FBP1 and hypoxia-related gene expression in clear cell renal cell carcinoma

Association between FBP1 and hypoxia-related gene expression in clear cell renal cell carcinoma

TPX2 in human clear cell renal carcinoma: Expression, function and prognostic significance

Forkhead box J1 expression is upregulated and correlated with prognosis in patients with clear cell renal cell carcinoma

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