Application of single hair analysis reveals multiple administration of a drug mixture in a case of drug‐facilitated sexual assault of a child

Wiedfeld, Christopher; Skopp, Gisela; Kuepper, Uta; Mußhoff, Frank

doi:10.1002/dta.3057

Cited by 5 publications

(4 citation statements)

References 12 publications

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“…As already reported in our previous publications, a representative number of at least 10 hairs should be analyzed to consider the presence of telogen hairs and to compensate influences due to intraindividual differences in hair growth rates. 8,9 In the present case, hair number 8 might be in the telogen state due to amphetamine being detectable over its complete length, whereas no amphetamine could F I G U R E 1 Schematic timeline showing the most important time points and events. The time is indicated in days before hair sampling.…”

Section: Discussionmentioning

confidence: 78%

“…So a rate of 1.1 cm/month (0.36 mm/day) was applied being a representative growth rate 7 . As already described in our previous publications, an offset value must be considered for back calculation in single hair analysis 8,9 . Due to a reported residual hair length of 1 mm and the intradermal part of the hair (about 4 mm 12 ), a section of at least 5 mm is not covered by the analysis corresponding to a period of approximately 14 days, which must be considered.…”

Section: Discussionmentioning

confidence: 99%

“…Resolution of several weeks to 1 month can be achieved by routine methods using segments of 0.5–1 cm 7 . To enhance the temporal resolution, single hair analysis can provide further information in special cases 8 . A previously presented fully validated multi‐analyte screening method for single hair analysis 9 was applied to a doping case involving amphetamine to further evaluate the results obtained by routine segmental analysis.…”

Section: Introductionmentioning

confidence: 99%

“…7 To enhance the temporal resolution, single hair analysis can provide further information in special cases. 8 A previously presented fully validated multi-analyte screening method for single hair analysis 9 was applied to a doping case involving amphetamine to further evaluate the results obtained by routine segmental analysis. Investigative procedures gave rise to the question whether amphetamine was accidentally ingested over a longer period or taken voluntarily on several occasions.…”

mentioning

confidence: 99%

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Application of single hair analysis in a doping case involving amphetamine

Wiedfeld

Skopp

Thieme³

et al. 2022

Drug Testing and Analysis

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Hair analysis has become a valuable and widely accepted routine method in forensic and clinical toxicology, as well as in work-place drug testing. Main applications are the proof of abstinence or the evaluation of drug use during the past months. 1,2 However, hair is a rarely used specimen in doping control. So far, only blood and urine samples are considered in the Code of the World Anti-Doping Agency (WADA). 3 According to a consensus on hair testing for doping agents released by the Society of Hair Testing (SoHT), hair is not a suitable

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Section: Discussionmentioning

confidence: 78%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Application of single hair analysis in a doping case involving amphetamine

Wiedfeld

Skopp

Thieme³

et al. 2022

Drug Testing and Analysis

Self Cite

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Single hair analysis for gamma‐hydroxybutyric acid—Method optimization, validation, and application

Wiedfeld,

Skopp,

Musshoff

2024

Drug Testing and Analysis

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As gamma‐hydroxybutyric acid (GHB) underlies fast metabolization, its determination from hair may presumably offer a detection window superior to that of body fluids. Due to the wide range of endogenous concentration levels, the evidence of an exogenous ingestion is challenging. As already shown for other drugs, the temporal resolution obtained by applying single hair microanalysis provides further information. Therefore, a method for the extraction and quantification of GHB in 2‐mm hair segments (seg) was optimized and validated (limit of detection [LOD]: 2.5 pg/seg, lower limit of quantification [LLOQ]: 5 pg/seg), and five single hairs were examined, each for three non‐users and for three (alleged) users. A major challenge was the choice of appropriate extraction tubes without remains of GHB. In two samples from non‐users, GHB could not or could only be detected in trace amounts. In the third sample, concentrations between the LOD and 31.1 pg/seg (mean: 9.5, median: 8.4; each pg/seg) were detected with decreasing values towards the tips. In two samples of persons with assumed GHB intake, maximum concentrations of 6.8 and 30.7 pg/seg were measured, but no significant concentration peaks indicating a single ingestion could be observed. The third sample showed concentrations of 7.6–55.2 pg/seg (mean: 28.8, median: 29.6; each pg/seg). In this case, the obtained profiles showing at least two reproducible concentration maxima between 20 and 40 mm point to an ingestion of GHB. The concentration profiles from single hairs were reproducible in each case, reflecting the concentration course of routine 1‐cm segmental analysis. These are the first results published on GHB testing in segmented single hairs, and the results must be verified further.

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