Application of size-exclusion chromatography–inductively coupled plasma mass spectrometry for fractionation of element species in seeds of legumes

Koplík, Richard; Borková, Markéta; Mestek, Oto; Komínková, Jana; Suchánek, Miloslav

doi:10.1016/s1570-0232(02)00296-9

Cited by 29 publications

(23 citation statements)

References 12 publications

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“…The analysis of the standards used for species identification (Figure 3) showed that iodide, T3 and T4 are not separated strictly according to their hydrodynamic volumes, and they are retarded by other interactions with the stationary phase (the dependence of retention time t on molecular weight M is described by equation log M = −0.2287t + 9.5895). This phenomenon has been observed previously by other authors [42,43] working with Superdex columns. It is caused by the negatively charged groups bound on the stationary phase surface that are not sufficiently shaded when a mobile phase with a low ionic strength is used.…”

Section: Speciation Analysis Using Secsupporting

confidence: 87%

Analysis of iodine and its species in animal tissues

Kaňa

Hrubá

Vosmanská

et al. 2015

Chemical Speciation & Bioavailability

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A method allowing the determination of the total iodine content and iodine species in samples of animal tissues using inductively coupled plasma mass spectrometry (ICP-MS) as an elementspecific detector was developed. The total iodine content was determined after microwave digestion with 25% (w/w) water solution of tetramethylammonium hydroxide. The detection limit was 26.9 μg kg −1 I, and the accuracy of the determination was proven through the analysis of SRM "Non-Fat Milk Powder, " porcine liver, and Atlantic Cod muscle samples using standard addition methods. The extracts for the speciation analysis were prepared through sample dispersion in water using an Ultra-Turrax® T10. The extraction yields ranged from 46 to 84% for different types of tissues. The determination of the inorganic iodine species was performed using ion-exchange chromatography (PRP X100, mobile phase 100 mmol L −1 ammonium nitrate, pH 7.4) coupled to ICP-MS. A detection limit of 1.1 μg kg −1 I was obtained for both species. The organic iodine species were separated using size-exclusion chromatography (Superdex 75 column, mobile phase 20 mmol L −1 Tris-HCl, pH 7.5) and also detected using ICP-MS. Samples of porcine muscle, liver, kidney and thyroid gland, chicken muscle and liver and Atlantic Cod muscle were analyzed. The porcine thyroid gland and Atlantic Cod muscle samples were the richest in iodine (a more than 10× greater content of iodine than the other samples). With respect to the inorganic species, only iodide was found in the sample extracts. Conversely, many organic iodine species were found in the extracts.

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Section: Speciation Analysis Using Secsupporting

confidence: 87%

Analysis of iodine and its species in animal tissues

Kaňa

Hrubá

Vosmanská

et al. 2015

Chemical Speciation & Bioavailability

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“…Cabrera et al 11 reported Fe, Zn and Cu contents in kidney bean of 6.44, 4.69 and 3 mg per 100 g sample respectively. Koplik et al 12 found Cu and Zn contents in kidney bean of 0.67 and 3.18 mg per 100 g sample respectively as determined by inductively coupled plasma (ICP) spectroscopy after microwave‐assisted digestion with 3 mL of HNO 3 and 1 mL of H 2 O 2 .…”

Section: Resultsmentioning

confidence: 99%

Optimisation of microwave digestion for determination of Fe, Zn, Mn and Cu in various legumes by flame atomic absorption spectrometry

2005

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Fe, Zn, Mn and Cu levels in three Turkish legumes, kidney bean (Phaseolus vulgaris L.), lentil (Lens esculenta) and chickpea (Cicer arietinum), were determined by flame atomic absorption spectrometry. Dissolution conditions in the microwave-assisted wet digestion method were studied by investigating several variables, including type of acid mixture, acid volume, digestion time, microwave power input and sample weight. Comparison with conventional wet acid digestion was also made. In order to check the element losses during digestion and the accuracy of the results, all tests were repeated after the addition of a spiked standard element solution to the legume sample. The microwave-assisted digestion procedure optimised for kidney bean was adapted for lentil and chickpea. Fe, Zn, Mn and Cu concentrations (mg per 100 g sample) were determined in kidney bean as 6.27 ± 0.94, 2.23 ± 0.36, 1.64 ± 0.14 and 0.99 ± 0.19, in lentil as 8.24 ± 1.11, 2.46 ± 0.06, 1.17 ± 0.19 and 1.01 ± 0.28 and in chickpea as 6.00 ± 1.40, 2.21 ± 0.14, 1.60 ± 0.43 and 0.58 ± 0.18 respectively.

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“…Other elements targeted in species analysis in marine organisms include selenium [21][22][23], copper, zinc and cadmium [24]. The distribution of selenium between fractions with different MW has been presented in different fish species [25,26], while some investigations of soluble element species in food (such as soybean flour, pea and lentil seeds and white bean seeds) were reported by Koplík et al [27,28], in which data were presented on the fractionation of Mn, Fe, Co, Ni, Cu, Zn, Se, Mo and P species in different MW ranges.…”

Section: Introductionmentioning

confidence: 99%