Application of sodium silicate retards apple softening by suppressing the activity of enzymes related to cell wall degradation

Ge, Yonghong; Duan, Bin; Li, Canying; Wei, Meilin; Chen, Yanru; Li, Xue; Tang, Qi

doi:10.1002/jsfa.9376

Cited by 43 publications

(21 citation statements)

References 39 publications

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“…Fruit firmness is a vital factor affecting the shelf life of fresh fruit (Ge, Duan, et al, ). Generally, flesh firmness decreases due to water loss, and cell degradation during storage with the action of cell wall‐degrading enzymes.…”

Section: Discussionmentioning

confidence: 99%

“…Crude enzymes were extracted according to the method described by Ge, Duan, et al (). Three grams of flesh tissues were ground in 5.0‐ml 95% ethanol then centrifuged for 15 min at 4°C (12,000× g ).…”

Section: Methodsmentioning

confidence: 99%

“…The method of Ge, Duan, et al () was employed to assay PME and PG activities. The activity of PME and PG was expressed as U.…”

Section: Methodsmentioning

confidence: 99%

“…Cellulase activity was determined following Ge, Duan, et al () method and the result was expressed as U, where 1 U represented the production of 1 μg of glucose g −1 FW/hr. The method of Wei et al () with slight modifications was applied to determine β‐glu activity.…”

Section: Methodsmentioning

confidence: 99%

“…Loss of fruit firmness was due to pectin, cellulose, and hemicellulose degradation under the action of enzymes including pectin methylesterase (PME), polygalacturonase (PG), pectin lyase (PL), cellulase, and β‐glucosidase (β‐glu). Studies have demonstrated that decrease in flesh firmness could be effectively delayed by the application of heat treatment (Martinez & Civello, ), UV‐C (Barka, Kalantari, Makhlouf, & Arul, ; Wei et al, ), sodium silicate (Ge, Duan, et al, ), 1‐methylcyclopropene (Tatsuki, Endo, & Ohkawa, ), nitric oxide (NO) fumigation (Zhu, Sun, & Jie, ), chitosan coating (Xing et al, ), and calcium chloride (Ranjbar, Rahemi, & Ramezanian, ). The most fundamental mechanism is that these exogenous treatments inhibited cell wall‐degrading enzymes activities and delayed the decomposition of cell walls during storage.…”

Section: Introductionmentioning

confidence: 99%

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Postharvest acibenzolar‐S‐methyl treatment maintains storage quality and retards softening of apple fruit

Zhang

et al. 2020

J Food Biochem

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“Golden delicious” apples were dipped in 100 mg/L acibenzolar‐S‐methyl (ASM) to investigate the fruit quality and softening during 12 days of storage. Weight loss, flesh firmness, ethylene release, respiratory rate, content of total soluble solids and titratable acid, the activity of pectinase, cellulase, and β‐glucosidase, and water‐insoluble pectin and water‐soluble pectin contents were investigated. The results demonstrated that ASM treatment inhibited ethylene release and respiratory rate, reduced titratable acidity, and enhanced total soluble solids content in apples. Moreover, application of ASM suppressed the reduction of flesh firmness, activity of pectin methylesterase, and polygalacturonase. Cellulase, β‐glucosidase, and degradation of protopectin in apple fruit were also suppressed by ASM treatment during storage. In conclusion, ASM could maintain fruit quality by regulating cell wall‐degrading enzymes during storage. Practical applications Application of acibenzolar‐S‐methyl after harvest has the potential of delaying fruit softening by regulating cell wall‐degrading enzymes, thus retain fruit quality.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…The method of Ge, Duan, et al () was employed to assay PME and PG activities. The activity of PME and PG was expressed as U.…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Postharvest acibenzolar‐S‐methyl treatment maintains storage quality and retards softening of apple fruit

Zhang

et al. 2020

J Food Biochem

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Cell wall modification and lignin biosynthesis involved in disease resistance against Diaporthe citri in harvested pummelo fruit elicited by carvacrol

et al. 2021

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BACKGROUND Phomopsis stem‐end rot caused by Diaporthe citri, causes significant commercial postharvest losses of pummelo fruit during storage. Carvacrol (CVR) is a known generally recognized as safe and has the ability to prolong the preservation of harvested fruits. In the present study, the inhibitory effects of CVR treatment at the appropriate concentration on Phomopsis stem‐end rot development of harvested pummelo fruit inoculated with D. citri were evaluated by the amounts of cell wall components, the activities and gene expressions of related enzymes involved in cell wall modification and lignin biosynthesis. RESULTS Results indicated that CVR completely inhibited D. citri growth in vitro at 200 mg L−1 and significantly controlled Phomopsis stem‐end rot development in harvested pummelo. The CVR treatment delayed peel softening and browning, and retarded electrolyte leakage, superoxide radical (O2•−) production, and malondialdehyde content. The CVR‐treated fruit maintained higher amounts of cell wall material, protopectin, hemicelluloses, and cellulose, but exhibited lower water‐soluble pectin amount. Moreover, in D. citri‐inoculated fruit, CVR treatment suppressed the activities and gene expressions of cell wall disassembling‐enzymes, including pectin methylesterase, polygalacturonase, cellulase, and β‐galactosidase, while the development of cell wall degradation was reduced. Meanwhile, the CVR treatment enhanced the lignin biosynthesis by increasing the activities and up‐regulating the gene expressions of phenylalanine ammonialyase, cinnamic alcohol dehydrogenase, and peroxidase accompanied with elevated level of lignin in pummelo fruit. CONCLUSION The disease resistance to D. citri in pummelo fruit elicited by CVR treatment is related to delaying cell wall degradation and enhancing lignin biosynthesis. © 2021 Society of Chemical Industry.

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Cooperative effects of three preservatives on physiological quality, endophytic bacterial community and volatile organic compounds of postharvest Codonopsis pilosula var. modesta roots

Wang,

Wen,

Tao

et al. 2024

J Sci Food Agric

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BACKGROUNDCodonopsis pilosula var. modesta (CPVM) is a famous medicinal and edible plant of Campanulaceae. However, fresh CPVM roots (FCPVR) are prone to softening, browning and spoilage after concentrated harvesting in the main production area of Gansu Province, China in autumn, which poses great challenges to their large‐scale storage and modern processing. In this study, effects of chitosan (CS), natamycin (NA) and modified atmosphere agent (MA) on the postharvest quality of FCPVR were first investigated. The roots after different treatments were stored at 4 °C and relative humidity of 75 ± 5% for 100 days. Their overall quality changes were evaluated from three perspectives: physiological quality, endophytic bacterial community and volatile organic compounds.RESULTSThe clustering heatmap and principal component analysis results indicated that CS (2 g kg−1), NA (0.5 g kg−1) and MA (5 g) had a synergistic effect on physiological quality. The roots in the CS + NA + MA group maintained better physiological state, effective components and antioxidant capacity throughout the storage process. On this basis, compared with room temperature storage, the relative abundance of the main spoilage bacterium Pseudomonas in the CS + NA + MA group roots decreased by 44% on the 100th day of storage. Furthermore, after CS + NA + MA composite treatment, the roots produced richer esters with fruit aroma during low‐temperature storage.CONCLUSIONSThe CS + NA + MA composite treatment could maintain the physiological quality and flavor of FCPVR, inhibit spoilage by microbial contamination and maintain the optimal quality during low‐temperature storage for up to 100 days. © 2024 Society of Chemical Industry.

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Application of sodium silicate retards apple softening by suppressing the activity of enzymes related to cell wall degradation

Cited by 43 publications

References 39 publications

Postharvest acibenzolar‐S‐methyl treatment maintains storage quality and retards softening of apple fruit

Postharvest acibenzolar‐S‐methyl treatment maintains storage quality and retards softening of apple fruit

Cell wall modification and lignin biosynthesis involved in disease resistance against Diaporthe citri in harvested pummelo fruit elicited by carvacrol

Cooperative effects of three preservatives on physiological quality, endophytic bacterial community and volatile organic compounds of postharvest Codonopsis pilosula var. modesta roots

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