Application of sol-gel glass immunoadsorbers for the enrichment of polycyclic aromatic hydrocarbons (PAHs) from wet precipitation

Scharnweber, Tim; Knopp, Dietmar

doi:10.1002/(sici)1520-6521(2000)4:1<43::aid-fact5>3.0.co;2-i

Cited by 20 publications

(3 citation statements)

References 19 publications

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“…The preparation of immunoaffinity columns by using the sol-gel method offers several advantages compared to covalently binding the antibodies to a solid support material; for example, the synthesis is Because the entrapment of antibodies in the pores of the solgel glass is carried out under mild conditions, the affinity of the antibody to the antigen is largely retained. Sol-gel immunoaffinity columns have been shown to efficiently remove interfering matrix compounds from food (22,23) and environmental (24)(25)(26) as well as urine samples (27).…”

Section: Introductionmentioning

confidence: 99%

“…Because the entrapment of antibodies in the pores of the sol−gel glass is carried out under mild conditions, the affinity of the antibody to the antigen is largely retained. Sol−gel immunoaffinity columns have been shown to efficiently remove interfering matrix compounds from food ( , ) and environmental ( − ) as well as urine samples ().…”

Section: Introductionmentioning

confidence: 99%

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Determination of Bisphenol A in Canned Foods by Immunoaffinity Chromatography, HPLC, and Fluorescence Detection

Braunrath

Podlipna

Padlesak

et al. 2005

J. Agric. Food Chem.

128

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Bisphenol A (BPA) concentrations were determined in canned beverages, fruits, vegetables, and fat-containing foodstuffs bought in Austrian supermarkets. The analysis method consisted of sol-gel immunoaffinity chromatography followed by high-performance liquid chromatography with fluorescence detection. With one exception traces of BPA were detected in all samples. BPA recovery strongly depended on the food matrix, ranging from 27% in goulash to 103% in a lemon soft drink. The results obtained allow a more realistic picture of the BPA exposure caused by cans with an epoxy resin protective coating because--in contrast to several previous studies--only those fractions of the can contents that are actually consumed were analyzed. BPA concentrations ranging from 0.1 ng/mL (lemon soft drink) to 38 ng/g (ready-to eat soup from Thailand) were significantly lower than the European Union migration limit of 0.6 mg of BPA/kg of food.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Determination of Bisphenol A in Canned Foods by Immunoaffinity Chromatography, HPLC, and Fluorescence Detection

Braunrath

Podlipna

Padlesak

et al. 2005

J. Agric. Food Chem.

128

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show abstract

“…have been successfully encapsulated in glasses of silica or other metal oxides by sol-gel techniques (9). Sol-gel IS for some organic pollutants have also been synthesized (10)(11)(12)(13)(14)(15)(16), but only a few works report their application for selective trace enrichment from real environmental samples (17)(18)(19)(20)(21)(22).…”

Section: Introductionmentioning

confidence: 99%

Sol−Gel Immunosorbents Doped with Polyclonal Antibodies for the Selective Extraction of Malathion and Triazines from Aqueous Samples

Vera-Avila

Vazquez-Lira

Llasera

et al. 2005

Environ. Sci. Technol.

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Sol-gel immunosorbents (IS) prepared by encapsulation of polyclonal antibodies in silica were packed in cartridges and evaluated for selective immunoaffinity extraction (IAE) of malathion and triazines from aqueous samples. Encapsulated atrazine antibodies highly cross-reacted with simazine and propazine but did not recognize prometon and prometryn. No cross-reactivity of malathion antibodies was observed with the closely related metabolites oxomalathion and isomalathion. Mean IS binding capacities per milligram of entrapped antibody were 0.33 nmol of malathion and 0.47 nmol of atrazine (approximately 100 ng each). This capacity remained constant for at least 10 weeks, and the cartridge reusability was excellent (>60 IAE runs); also, high preconcentration factors were feasible because the breakthrough of analytes from IS cartridges did not occur up to the 250 mL sample volumes, provided that the capacity was not surpassed. Simple and rapid methods for determination of malathion or three triazines in surface water were developed using off-line IAE and HPLC-UV. The application to 50 mL dam water samples spiked at approximately 1 ng/mL of pesticides resulted in recoveries of approximately 90% and RSD < 5% (n=7). LODs for this sample volume (direct injection of IS eluates) were in the range of 0.15-0.50 ng/mL. Lower LODs (0.03-0.1 ng/mL) were achieved by online analysis of whole eluates previously loaded in RP precolumns.

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Enzyme and Immunoassays

Miller¹,

Knopp²

2001

Ullmann's Encyclopedia of Industrial Chemistry

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Application of sol-gel glass immunoadsorbers for the enrichment of polycyclic aromatic hydrocarbons (PAHs) from wet precipitation

Cited by 20 publications

References 19 publications

Determination of Bisphenol A in Canned Foods by Immunoaffinity Chromatography, HPLC, and Fluorescence Detection

Determination of Bisphenol A in Canned Foods by Immunoaffinity Chromatography, HPLC, and Fluorescence Detection

Sol−Gel Immunosorbents Doped with Polyclonal Antibodies for the Selective Extraction of Malathion and Triazines from Aqueous Samples

Enzyme and Immunoassays

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