Application of solid phase extraction for profiling quinolizidine alkaloids and phenolic compounds in Lupinus albus

Stobiecki, Maciej; Wojtaszek, Przemysław; Gulewicz, Krzysztof

doi:10.1002/(sici)1099-1565(199707)8:4<153::aid-pca356>3.3.co;2-7

Cited by 17 publications

(17 citation statements)

References 18 publications

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“…Samples of plant extracts for LC analyses were prepared from 0.5 g FW pea tissue. The samples were purified and concentrated by solidphase extraction on cartridges containing a cation exchanger and RP C-18 silica gel (Alltech, Carnforth, England) used in tandem, according to the method of Stobiecki et al (1997).…”

Section: Isolation Of Phenolic Compoundsmentioning

confidence: 99%

Pea aphid infestation induces changes in flavonoids, antioxidative defence, soluble sugars and sugar transporter expression in leaves of pea seedlings

et al. 2015

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The perception of aphid infestation induces highly coordinated and sequential defensive reactions in plants at the cellular and molecular levels. The aim of the study was to explore kinetics of induced antioxidative defence responses in leaf cells of Pisum sativum L.cv. Cysterski upon infestation of the pea aphid Acyrthosiphon pisum at varying population sizes, including accumulation of flavonoids, changes of carbon metabolism, and expression of nuclear genes involved in sugar transport. Within the first 96 h, after A. pisum infestation, flavonoid accumulation and increased peroxidase activity were observed in leaves. The level of pisatin increased after 48 h of infestation and reached a maximum at 96 h. At this time point, a higher concentration of flavonols was observed in the infested tissue than in the control. Additionally, strong post-infestation accumulation of chalcone synthase (CHS) and isoflavone synthase (IFS) transcription products was also found. The levels of sucrose and fructose in 24-h leaves infested by 10, 20, and 30 aphids were significantly lower than in the control. Moreover, in leaves infested by 30 aphids, the reduced sucrose level observed up to 48 h was accompanied by a considerable increase in the expression level of the PsSUT1 gene encoding the sucrose transporter. In conclusion, A. pisum infestation on pea leads to stimulation of metabolic pathways associated with defence.

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Section: Isolation Of Phenolic Compoundsmentioning

confidence: 99%

Pea aphid infestation induces changes in flavonoids, antioxidative defence, soluble sugars and sugar transporter expression in leaves of pea seedlings

et al. 2015

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“…Natural products are extracted by conventional methods such as Soxhlet and room temperature solvent extraction, [1][2][3][4][5][6][7] or by ultrasound, [8][9][10][11][12][13][14] microwaves, [15][16][17][18] supercritical solvents [19][20][21][22][23][24][25][26][27][28][29][30] or other methods. [31][32][33][34] In this paper a new method is developed to extract alkaloids, which are among the most important groups of secondary metabolites.…”

Section: Introductionmentioning

confidence: 99%

New extraction technique for alkaloids

Djılanı¹,

Legseir²,

Soulimani³

et al. 2006

J. Braz. Chem. Soc.

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Um método de extração de produtos naturais foi desenvolvido. Comparado aos outros métodos, a nova técnica é rápida, mais eficiente e consome menos solvente. A extração de alcalóides de produtos naturais, como Hyoscyamus muticus, Datura stramonium e Ruta graveolens, consiste no uso de uma solução sonicada, contendo um surfactante como agente extrator. Os alcalóides são precipitados pelo reagente de Mayer, dissolvidos em uma solução alcalina, e então, extraídos com clorofórmio. Este artigo compara os resultados obtidos com outros métodos, mostrando claramente as vantagens do novo método.A method of extraction of natural products has been developed. Compared with existing methods, the new technique is rapid, more efficient and consumes less solvent. Extraction of alkaloids from natural products such as Hyoscyamus muticus, Datura stramonium and Ruta graveolens consists of the use of a sonicated solution containing a surfactant as extracting agent. The alkaloids are precipitated by Mayer reagent, dissolved in an alkaline solution, and then extracted with chloroform. This article compares the results obtained with other methods showing clearly the advantages of the new method.

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“…Prior to LC profiling of glycosylated flavonoids, the frozen soybean leaves (0.5 g) were homogenized in 80 % methanol (20 mL g -1 FW) and sonicated for 3 min using a Bransonic 5510 bath sonicator. The suspension was filtered through a Büchner funnel and concentrated under vacuum at 40 o C. The samples were concentrated by solid phase extraction (SPE) on cartridges containing a cation exchanger and RP C18 silica gel (Alltech, Carnforth, England) [5].…”

Section: Analysis Of Glycosylated Flavonoidsmentioning

confidence: 99%

Changes in glycosylated flavonoids in soybean (Glycine max (L.) Merr. cv. “Nam Dan”) leaves under aphid infestation

Thơm

Toan

Huyền

et al. 2018

JST

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Changes in contents of glycosylated flavonoids in leaves of soybean (Glycine max (L.) Merr. cv. “Nam Dan”) in the flowering stage under infestation of cowpea aphid (Aphis craccivora Koch) was investigated. A strong decrease in the level of glycosylated flavonoids, e.g., genistein 7-O-glucoside, 2’OH genistein 7-O-glucoside, 2’OH genistein malonyl glucoside, after 48-96 hours post-infestation (hpi) of aphid. Levels of these compounds in aphid-infested leaves were remarkably lower than those in controls. Whereas, genistein and total flavonoids in the aphid-infested leaves were induced to higher contents than that observed in control. Activity of important enzymes regarding biosynthesis of flavonoids such as phenylalanine ammonia-lyase (PAL, EC. 4.3.1.5), chalcone synthase (CHS, EC 2.3.1.74) and chalcone isomerase (CHI, EC 5.5.1.6) in the aphid-infested leaves strongly increased during experiemntal time. An increase in acitivity of those enzymes was proportional with the induced decrease of the glycosylated flavonoids and an accumulated increase of genistein and total flavonoids. These results indicate that, changes in the glycosylated flavonoids occured in soybean “Nam Dan” leaves infested by cowpea aphid may connect to a protective mechanism in this soybean cultivar in the flowering stage.

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Application of solid phase extraction for profiling quinolizidine alkaloids and phenolic compounds in Lupinus albus

Cited by 17 publications

References 18 publications

Pea aphid infestation induces changes in flavonoids, antioxidative defence, soluble sugars and sugar transporter expression in leaves of pea seedlings

Pea aphid infestation induces changes in flavonoids, antioxidative defence, soluble sugars and sugar transporter expression in leaves of pea seedlings

New extraction technique for alkaloids

Changes in glycosylated flavonoids in soybean (Glycine max (L.) Merr. cv. “Nam Dan”) leaves under aphid infestation

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