Application of solid-phase extraction for isolation of coumarins from wine samples

Abstract

Coumarins can be as a result wine storage and aging in wood drums and they can affect organoleptic characteristics of wine. The aim of this work was to determine the content of coumarins in wine samples originated from Slovak Tokaj wine region. The HPLC method with high specificity, accuracy, precision, and recovery was proposed. SPE sorbents of C18 type, styrene-divinylbenzene copolymer and molecularly imprinted polymers were compared for extraction of six coumarins, coumarin, aesculin, scoparone, scopoletin, 4-methylumbelliferone, and herniarin. Higher recoveries (above 89 %; except aesculin – recoveries higher from 68 %, RSDs less than 6 %) were obtained with selective polymeric sorbent laboratory prepared by molecularly imprinted technology. The results showed that content of coumarins in wine samples are in ng.mL−1 concentration levels and depend on the age and wine puttony.

The Application of Pipette-Tip and Magnetic Dummy-Template Molecularly Imprinted Solid-Phase Extraction Coupled with High-Performance Liquid Chromatography with Diode Array and Spectrofluorimetric Detection for the Determination of Coumarins in Cosmetic Samples

“…MIP-based extraction allows for a higher recovery for targeted compounds, e.g., bisphenol A, parabens, drugs, the antibacterial and antifungal agent triclosan, and others. The extraction of coumarins by MISPE was used in an analysis of wines and plant extracts [38,39].…”