Application of spectrophotometric, densitometric, and HPLC techniques as stability indicating methods for determination of Zaleplon in pharmaceutical preparations

Metwally, Fadia H; Abdelkawy, Mohamed; Abdelwahab, Nada S.

doi:10.1016/j.saa.2007.01.024

Cited by 16 publications

(11 citation statements)

References 17 publications

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“…The amounts of compounds are determined by comparing the peak area of the unknown band to a standard curve from reference materials chromatographed under the same condition [18]. The developed TLC-densitometric method depends on the difference in R f values of AB (R f =0.21) and PA (R f =0.31) (Figure 3).…”

Section: Resultsmentioning

confidence: 99%

Validated Chromatographic Methods for Simultaneous Determination of Amlodipine Besylate and Perindopril Arginine in Binary Mixtures and in Pharmaceutical Dosage Form

Ali¹,

Abdelwahab²

2012

J Chromat Separation Techniq

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Two accurate, selective and precise chromatographic methods, namely TLC-densitometric method & RP-HPLC method & were developed and validated for the simultaneous determination of Amlodipine besylate and Perindopril Arginine in their binary mixtures.The developed TLC-densitometric method depends on separation and quantitation of the studied drugs on silica gel 60F 254 TLC plates. Chloroform:methanol:deionized water:glacial acetic acid:triethylamine (10:7:5:0.3:0.2, by volume) was used as developing system and the separated bands were scanned at 208 nm. Linear relationship was obtained in the range 1-10 µg for both drugs.The developed RP-HPLC depends on quantitative chromatographic separation of the studied drugs on a C18 column using phosphate Buffer:acetonitrile (60:40, v/v), pH=4.6 as a mobile phase delivered at constant flow rate of 1 mL.min -1 with UV detection at 210 nm. Calibration curves for Amlodipine besylate and Perindopril Arginine were constructed over the concentration range of 1-50 µg.mL -1 for both drugs.The proposed methods were successfully applied for determination of the studied drugs in their bulk powder and in pharmaceutical formulations. The proposed methods were statistically compared to each other using student's t test and F-test and no significant difference was found between them.

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Section: Resultsmentioning

confidence: 99%

Validated Chromatographic Methods for Simultaneous Determination of Amlodipine Besylate and Perindopril Arginine in Binary Mixtures and in Pharmaceutical Dosage Form

Ali¹,

Abdelwahab²

2012

J Chromat Separation Techniq

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“…Nanoparticles were separated by ultracentrifugation (Sigma Laborzentrifugen GmbH., Germany) at 20,000 rpm for half an hour. The concentration of free Za in the collected supernatant was determined by a reported high-pressure liquid chromatography (HPLC) method with some modifications 25 . The HPLC system consists of autosampler (Waters ® 717), controller (Waters ® 600) and tunable absorbance UV detector (Waters ® 486).…”

Section: Entrapment Efficiencymentioning

confidence: 99%

Development and Optimization of Zaleplon Loaded Eudragit® Nanoparticles for Oral Delivery: In-Vitro and in-Vivo Evaluation

El‐Gizawy¹,

Essa²,

Abosalha³

2019

Int Res J Pharm

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Polymeric nanoparticles (PNPs) have a great potential as drug delivery systems regarding their small size, high physicochemical stability, and sustainedrelease properties. Zaleplon (Za) is a model BCS class II drug with sedative and hypnotic actions. Zaleplon loaded polymeric nanoparticles (Za-NPs) were prepared from Eudragit ® RS100 using a single emulsion solvent evaporation technique. Full factorial design (2 3) was applied to optimize the physicochemical characterization of Za-NPs. The three independent variables were the amount of polymer, the concentration of PVA and the volume of organic phase. The effects of these variables on particle size (PS), zeta potential (ZP), entrapment efficiency (%EE) and in vitro drug release were investigated. In-vivo study was conducted in rats to evaluate the pharmacological effects of the selected Za-NPs formulations. The designed formulations of Za-NPs had a spherical shape with a size range of 146 to 250 nm and a positive zeta potential in the range of 17.7 to 39.3 mv. The % EE of Za-NPs formulations were in the range of 66.3 to 87.6%. Differential thermal analysis (DTA) showed no interaction between Zaleplon and other excipients meanwhile a change in drug crystallinity was observed. Za-NPs showed a significant increase in the sedative effects in vivo compared with the free drug. Stability studies of the selected lyophilized Za-NPs formulations indicated a high stability after 3 months.

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“…In modern analytical laboratory, there is always a need for significant stability indicating methods of analysis [1,2]. An ideal stability indicating method quantifies a drug and resolves its degradation products [3].…”

Section: -Introductionmentioning

confidence: 99%

“…The method of Kaiser[22] was used for the selection of optimum wavelength set which assured the best sensitivity for the determination. A series of sensitivity matrices, K, was created for each binary mixture and for every pair of preselected wavelengths: A1,2 and m B1,2 are the slopes of the components A and B at the two selected wavelengths(1,2).The determinants of these matrices were ϭϭ calculated and the wavelength set selected for which the highest matrix determinant value was obtained.…”

mentioning

confidence: 99%

A comparative study between three stability indicating spectrophotometric methods for the determination of diatrizoate sodium in presence of its cytotoxic degradation product based on two-wavelength selection

Riad

El-Rahman

Fawaz

et al. 2015

Spectrochimica Acta Part A: Molecular and Biomolecular Spectros

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Application of spectrophotometric, densitometric, and HPLC techniques as stability indicating methods for determination of Zaleplon in pharmaceutical preparations

Cited by 16 publications

References 17 publications

Validated Chromatographic Methods for Simultaneous Determination of Amlodipine Besylate and Perindopril Arginine in Binary Mixtures and in Pharmaceutical Dosage Form

Validated Chromatographic Methods for Simultaneous Determination of Amlodipine Besylate and Perindopril Arginine in Binary Mixtures and in Pharmaceutical Dosage Form

Development and Optimization of Zaleplon Loaded Eudragit® Nanoparticles for Oral Delivery: In-Vitro and in-Vivo Evaluation

A comparative study between three stability indicating spectrophotometric methods for the determination of diatrizoate sodium in presence of its cytotoxic degradation product based on two-wavelength selection

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