Application of SPR biosensor for medical diagnostics of human hepatitis B virus (hHBV)

Chung, Jin; Kim, S.D.; Bernhardt, Rita; Pyun, Jae Chul

doi:10.1016/j.snb.2005.03.055

Cited by 158 publications

(77 citation statements)

References 22 publications

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“…Addition to enhance the absorption of biomelecule or enzyme, the SPR sensing surface will be modified by thin polymer layer [14,15]. Moreover, the covalent binding between antigen and antibody has also a powerful method for biosensors such as detection of metamphetamine, detection of low molecular weight domoic acid, medical diagnostics of human hepatitis B virus (hHBV), assay of the different poliovirus types [16][17][18][19][20]. Another important field is study an immobilization of tTGas relating to the Ca 2+ concentration, which is so important in human health [21].…”

Section: Surface Plasmon Resonance Sensors For Disease Diagnosis 2/3mentioning

confidence: 99%

Surface Plasmon Resonance Sensors for Disease Diagnosis

Nguyen¹

2017

IJBSBE

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Section: Surface Plasmon Resonance Sensors For Disease Diagnosis 2/3mentioning

confidence: 99%

Surface Plasmon Resonance Sensors for Disease Diagnosis

Nguyen¹

2017

IJBSBE

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“…After the modification of the electrode, E. coli was immobilized on the modified surface at a concentration of 1 × 10 9 cells/ml (OD 578 = 1.0) for 4 h and then washed three times with 10 mM HEPES buffer. Finally, the electrode was transferred onto the electrochemical cell for measurement [3,7,8]. The background buffer solution (10 mM HEPES) in the electrochemical cell was carefully bubbled with argon to remove the oxygen before measurement.…”

Section: Electrochemical Analysismentioning

confidence: 99%

“…In this work, E. coli cells with autodisplayed Adx were immobilized onto gold electrodes modified with a self-assembled monolayer of mercaptoundecanoic acid (MUA) for the determination of the redox potentials of the iron-sulfur cluster of the autodisplayed Adx [3,7,8]. The oxidation and reduction peaks were estimated from the repeated CV analysis and the iron-sulfur cluster of the autodisplayed Adx were observed to undergo reversible redox reactions via direct electron transfer to the MUA-modified gold electrode.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical analysis of autodisplayed adrenodoxin (Adx) on the outer membrane of E. coli

Yoo

Bong

Park

et al. 2015

Biochimica et Biophysica Acta (BBA) - Biomembranes

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In this work, adrenodoxin (Adx) was expressed on the outer membrane of E. coli by autodisplay and then the iron-sulfur cluster was incorporated into apo-Adx by an anaerobic reconstitution process. For the determination of the redox potentials of the iron-sulfur clusters of the autodisplayed Adx, E. coli cells with autodisplayed Adx were immobilized on a gold electrode modified with a self-assembled monolayer of mercaptoundecanoic acid (MUA). From the repeated cyclic voltammetry (CV) analysis, the E. coli (10mM HEPES buffer, pH7.0) with autodisplayed Adx showed significant changes in shape with an oxidation peak at +0.4V (vs. Ag/AgCl) and a reduction peak at -0.3V (vs. Ag/AgCl) after the reconstitution process for the incorporation of the iron-sulfur cluster. From the repeated CV analysis in the reduction and oxidation potential ranges, the iron-sulfur clusters of the autodisplayed Adx were observed to undergo reversible redox reactions via direct electron transfer to the MUA-modified gold electrode.

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“…The detection system involved an initial immunomagnetic bead-based separation step of 30 min and had a sensitivity of 10 6 CFU/ml in a total assay time of 2 h. A SAM-based SPR immunosensor was developed by Jyoung et al for the detection of V. cholerae O1 with a detection range of 10 5 to 10 9 cells/ml [62]. Detection of viruses using SPR-based biosensors have been reported by Chung et al [63], Vaisocherova et al [64]. Vaisocherova and coworkers developed an SPR biosensor for detecting antibodies against the Epstein-Barr virus.…”

Section: Surface Plasmon Resonance Biosensors Surface Plasmon Resonamentioning

confidence: 99%

Microbiological Detectors for Food Safety Applications

Alocilja

Pal

2008

Wiley Handbook of Science and Technology for Homeland Security

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The use of microorganisms as biological weapons has long been reported in history. The most recent was the deliberate release of B. anthracis spores through the postal system in the United States in October 2001, resulting in 22 cases of anthrax and five deaths. Furthermore, there are up to 33 million cases of human illness each year from microbial pathogens in the food supply in the United States alone, with an associated cost of $2‐4 billion in 2006. The complexity of the US food supply chain from cradle to grave provides numerous entry points and routes in which (inadvertent and intentional) contaminants and pathogens can be introduced into the nation's food system. Many of these pathogenic microorganisms have a very low infectious dose to cause fatality. The most vulnerable populations are the very young, the elderly, and immunocompromised individuals. Although highly sensitive, current methods of detection are laborious, require highly skilled personnel, and typically require 2–7 days to obtain conclusive results. Their results are not available in the time‐scale desired in food defense, food quality assurance, or clinical laboratory procedures, which have safety, cost, and quality implications. Rapid on‐site point‐of‐care detection methods for pathogens have hence become a necessity. Biosensors can play a major role in addressing the combined needs for near real‐time diagnosis, specificity, sensitivity, portability, electronic data transmission, field‐based, ease of use, and low cost requirements. This chapter presents biosensors for rapid microbial detection in complex food matrices of concern to homeland security and food safety.

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Application of SPR biosensor for medical diagnostics of human hepatitis B virus (hHBV)

Cited by 158 publications

References 22 publications

Surface Plasmon Resonance Sensors for Disease Diagnosis

Surface Plasmon Resonance Sensors for Disease Diagnosis

Electrochemical analysis of autodisplayed adrenodoxin (Adx) on the outer membrane of E. coli

Microbiological Detectors for Food Safety Applications

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