Application of Statistical Design for the Production of Cellulase by Trichoderma reesei Using Mango Peel

Abstract

Optimization of the culture medium for cellulase production using Trichoderma reesei was carried out. The optimization of cellulase production using mango peel as substrate was performed with statistical methodology based on experimental designs. The screening of nine nutrients for their influence on cellulase production is achieved using Plackett-Burman design. Avicel, soybean cake flour, KH2PO4, and CoCl2 ·6H2O were selected based on their positive influence on cellulase production. The composition of the selected components was optimized using Response Surface Methodology (RSM). The optimum conditions are as follows: Avicel: 25.30 g/L, Soybean cake flour: 23.53 g/L, KH2PO4: 4.90 g/L, and CoCl2 ·6H2O: 0.95 g/L. These conditions are validated experimentally which revealed an enhanced Cellulase activity of 7.8 IU/mL.

Preparation and characterization of higher degree‐deacetylated chitosan‐coated magnetic adsorbent for the removal of chromium(VI) from its aqueous mixture

“…The predicted R 2 value of 0.9532 is in reasonable agreement with the adjusted R 2 of 0.9838 and the difference between the two is <0.2. Adequate precision measured value of 37.235 indicates an adequate signal and the model were navigated in the design space …”

Composition Optimization of the Nutrient Medium by Plakett-Berman Design for Increasing the Enzyme Activity of Cellulolytic Complex of Fennellia sp. 2806

“…Обговорення. Останнім часом метод Плакетта-Бермана отримав широке розповсюдження для оптимізації умов синтезу екстрацелюлярних гідролаз [21]. Більшість досліджень показали позитивні результати, проте цей метод використовували, головним чином, для підвищення целюлазної активності за одним з показників.…”

Biotechnological Potentialities and Valorization of Mango Peel Waste: A Review

“…Since mango peel is a rich source (10-15%) of pectin, a complex set of polysaccharides, pectinase treatment can be effective for release of fermentable sugars. Saravanan et al (2012) have suggested alkaline pretreatment to decrease the crystallinity of mango peel and for simultaneous removal of lignin and other inhibitors. For pretreatment, 2% NaOH was added and autoclaved at 121°C for 30 min and excess alkali was neutralized with phosphoric acid.…”

“…Mango peel at the level of 7% (w/v) has been used for carboxymethyl cellulose (CMCase) production using Paenibacillus polymyxa (Kumar et al 2012a). Mango peel has been used for the production of cellulase enzyme (Saravanan et al 2012) and a maximum cellulase activity of 7.8 IU/mL was reported under optimized conditions.…”