Application of statistical experimental design for optimisation of bioinsecticides production by sporeless Bacillus thuringiensis strain on cheap medium

Khedher, Saoussen Ben; Jaoua, Samir; Zouari, Nabil

doi:10.1590/s1517-83822013000300043

Cited by 6 publications

(3 citation statements)

References 23 publications

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“…The P-value serves as a tool for checking the significance of each coefficient and also important in understanding the mutual interactions between the variables. Smaller is the P-value, more significant the corresponding coefficient 13 . The coefficients of X 2 , X 3 , and had remarkable effects on ribonuclease production, followed by X 1 , X 4 and.…”

Section: Optimization Of Significant Variables For Rnase Production B...mentioning

confidence: 99%

Response Surface Methodology (RSM) Approach for Improved Extracellular RNase Production by a Bacillus sp.

Jamwal,

Kumar,

Sharma

et al. 2017

J. Adv. Mircobiol.

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The extracellular ribonuclease activity of Bacillus sp. was successfully improved by optimization of nutritional and physical parameters in a set of non-statistical and statistical experiments. Plackett-Burman and Central Composite Design in Response Surface Methodology were used to build statistical models to screen out the significant variables and then study the effect of such variables on ribonuclease production. Four significant variables namely malt extract, yeast extract, ammonium molybdate and fermentation broth pH were selected via 2 4 -factorial Central Composite Design (CCD) for ribonuclease production Bacillus sp. The optimized values obtained by the statistical analysis showed that malt extract 1. 40 % (w/v), yeast extract 2.12 % (w/v), ammonium molybdate 0.56 % (w/v) and pH 8.81 affected maximum ribonuclease production by Bacillus sp. The ribonuclease production after optimization increased up to 1.83-fold with 83.52 % yield in comparison to the conventional strategy. Analysis of variance (ANOVA) revealed high coefficient of determination (R 2 ) of 0.9268 for the respective responses at significant level (p < 0.05).

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Section: Optimization Of Significant Variables For Rnase Production B...mentioning

confidence: 99%

Response Surface Methodology (RSM) Approach for Improved Extracellular RNase Production by a Bacillus sp.

Jamwal,

Kumar,

Sharma

et al. 2017

J. Adv. Mircobiol.

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“…Factorial design technique was widely used to improve delta-endotoxin production [17,18]. A factorial experimental design was used to study the delta-endotoxin and proteolytic enzymes produced by B. thuringiensis subsp.…”

Section: Experimental Design Of Delta-endotoxin and Protease Yieldsmentioning

confidence: 99%

Statistical Analysis of Cultural Parameters Influencing Delta-Endotoxins and Proteases Productions by Bacillus thuringiensis kurstaki

2015

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Bacillus thuringiensis is a spore-forming bacterium that produces delta-endotoxins, which are toxic to lepidopterans, dipterans and coleopterans. Concomitantly with production of delta-endotoxins, B. thuringiensis produces proteolytic enzymes during growth and sporulation, affecting yields of toxins production. In this work, an experimental factorial design was employed for the dual evaluation of effects of delta-endotoxins and proteolytic enzyme on activity of a B. thuringiensis subsp. kurstaki strain. Main effects and interactions of three factors (soybean meal, starch and aeration) under two conditions (0 and 7 g/l NaCl) were analysed using statistical techniques. Regression models, known as useful tool to build relationships between variables and subsequently to make predictions, were suggested to fit the experimental data. Yields ranged between 6.10-15.76 × 10 −7 µg and 18.18-30.30 × 10 −7 IU for delta-endotoxin and proteolytic enzymes per cell, respectively. When NaCl (7 g/l) was added to the medium, yields ranged from 15.51 to 38.06 for delta-endotoxins and from 9.56 to 48.73 for proteases. Starch, soybean meal and aeration improved the delta-endotoxins production. Interactions (starch × air) and (soybean meal × air) showed a negative effect on deltaendotoxin yield, while (starch × soybean meal) had no effect. The active function of aeration was enhanced when NaCl (7 g/l) was added, and effects of all interactions on deltaendotoxins yield had been nullified. Soybean meal and aer-B Karim Ennouri ation had negative effects on proteolytic activity, in contrast to starch, (starch × air) and (soybean × air), which improved proteolytic activity yields. The inhibitory role of soybean meal shifted to positive effect according to the presence of NaCl (7 g/l) in the culture medium.

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“…Bacillus thuringiensis, B. popilliae, Lysinibacillus sphaericus, Paenibacillus larva, etc.) and viruses of nuclear polyhedrosis and granulosis are actively used as active substances [3][4][5][6][7]. The advantage of viral and bacterial insecticides is their environmental friendliness and cumulative effect in agrocenoses [8][9].…”

Section: Introductionmentioning

confidence: 99%

Entomopathogenic activity of bacterial and viral strains from the bioresource collection “State Collection of Entomoacariphages and Microorganisms”

et al. 2021

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As a result of a series of experiments to study the entomopathogenic activity of bacteria and viruses from the “State Collection of Entomoacariphages and Microorganisms”, it was revealed that the large wax moth Galleria mellonella L. it is sensitive to new strains of bacteria and viruses. In the future, the strains can be used in the subsequent stages of screening of microorganisms that are promising as agents of biological pest control. The maximum biological efficacy on the fifth day against the tested insect was observed when using bacterial strains Bacillus spp. BZR 1159 (94.6%) and BZR 936 (95.0%) and a granulovirus strain of the codling moth (CpGV) BZR L-5 (100%). Larvae of G. mellonella L were susceptible to melanization in the process of infection with new bacterial and viral agents.

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Application of statistical experimental design for optimisation of bioinsecticides production by sporeless Bacillus thuringiensis strain on cheap medium

Cited by 6 publications

References 23 publications

Response Surface Methodology (RSM) Approach for Improved Extracellular RNase Production by a Bacillus sp.

Response Surface Methodology (RSM) Approach for Improved Extracellular RNase Production by a Bacillus sp.

Statistical Analysis of Cultural Parameters Influencing Delta-Endotoxins and Proteases Productions by Bacillus thuringiensis kurstaki

Entomopathogenic activity of bacterial and viral strains from the bioresource collection “State Collection of Entomoacariphages and Microorganisms”

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