Application of super-resolution microscopy in mitochondria-dynamic diseases

Zou, Weiwei; Yang, Li; Lu, Hedong; Li, Min; Ji, Dongmei; Slone, Jesse; Huang, Taosheng

doi:10.1016/j.addr.2023.115043

Cited by 7 publications

(1 citation statement)

References 91 publications

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“…All samples imaged using CLSM can also be imaged using SIM, a powerful tool for live cell super-resolution imaging, which offers several advantages over other techniques, including lower requirements for fluorescent dyes, higher temporal resolution, and greater utilization of fluorescent photons. − Therefore, SIM is particularly suitable for live cell super-resolution imaging . Over the past decade, SIM has been extensively applied in subcellular-level studies, including cellular physiology and cellular dynamics. − Currently, subcellular structural imaging mainly relies on fluorescent proteins, organic fluorescence probes, and quantum dots. − In fact, proteins are difficult to degrade in the body, and metal ions may also have unknown toxicity to the human body. Therefore, structurally clear and pure organic fluorescence probes have significant potential in SIM super-resolution imaging for clinical application.…”

Section: Introductionmentioning

confidence: 99%

Ultrabright Xanthene Fluorescence Probe for Mitochondrial Super-Resolution Imaging

Wu,

Zhang,

Sha

et al. 2024

Anal. Chem.

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Mitochondria are important organelles that provide energy for cellular physiological activities. Changes in their structures may indicate the occurrence of diseases, and the super-resolution imaging of mitochondria is of great significance. However, developing fluorescent probes for mitochondrial super-resolution visualization still remains challenging due to insufficient fluorescence brightness and poor stability. Herein, we rationally synthesized an ultrabright xanthene fluorescence probe Me-hNR for mitochondria-specific superresolution imaging using structured illumination microscopy (SIM). The rigid structure of Me-hNR provided its ultrahigh fluorescence quantum yield of up to 0.92 and ultrahigh brightness of up to 16,000. Occupying the para-position of the O atom in the xanthene skeleton by utilizing the smallest methyl group ensured its excellent stability. The study of the photophysical process indicated that Me-hNR mainly emitted fluorescence via radiative decay, and nonradiative decay and inter-system crossing were rare due to the slow nonradiative decay rate and large energy gap (ΔE st = 0.55 eV). Owing to these excellent merits, Me-hNR can specifically light up mitochondria at ultralow concentrations down to 5 nM. The unprecedented spatial resolution for mitochondria with an fwhm of 174 nm was also achieved. Therefore, this ultrabright xanthene fluorescence probe has great potential in visualizing the structural changes of mitochondria and revealing the pathogenesis of related diseases using SIM.

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