Application of surface plasmon resonance biosensor for the detection of Candida albicans

Yodmongkol, Sirasa; Thaweboon, Sroisiri; Thaweboon, Boonyanit; Puttharugsa, Chokchai; Sutapun, Boonsong; Amarit, Ratthasart; Somboonkaew, Armote; Srikhirin, Toemsak

doi:10.7567/jjap.55.02be03

Cited by 12 publications

(5 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[53] Several factors can interfere in the C. albicans adhesion and biofilm formation, such as surface roughness, hydrophobicity, porosity, and surface energy. [54] All these factors are directly related with the surface properties of the material. Usually, low-energy hydrophobic surfaces are more prone to the biofilm formation, as well as rough surfaces that provides larger area for adhesion and proliferation of C. albicans.…”

Section: Discussionmentioning

confidence: 99%

Nanocomposites obtained by incorporation of silanized silver nanowires to improve mechanical properties and prevent fungal adhesion

Menezes¹,

Sampaio

Silva³

et al. 2021

Nano Select

View full text Add to dashboard Cite

In this work, the surfaces of silver nanowires (AgNWs) were modified by silanization process to improve the mechanical and antibiofilm properties of polymethylmethacrylate (PMMA) dental resins. A two-route methodology was used to silanize the AgNWs: first, a surface treatment using tetraethyl orthosilicate (TEOS) was performed to act as interface agent; and then, γmethacryloxypropyltrimethoxysilane (MPS) was used to insert functional groups compatible to the acrylic resin. PMMA/AgNWs composites were obtained using pristine (AgNW P ) and modified AgNWs (AgNW M ) at concentrations of 0.5 and 1.0 wt%. The mechanical (flexural and impact strength) and antibiofilm properties against Candida albicans was investigated. AgNW M showed an enhancement in the dispersion and adhesion within the PMMA matrix, which were responsible for improvements in the mechanical properties. Results of flexural strength showed significant improvements for the sample with incorporation of 0.5 wt% of AgNW M and no significative changes for the sample with AgNW P . In addition, a reduction of about 50% in the biofilm formation by C. albicans was observed for the samples with 0.5 and 1.0 wt% of AgNW M . The incorporation of silanized-AgNWs into PMMA dental resins can combine improvements in antimicrobial activity and in the flexural strength, being a potential antimicrobial biomaterial for dental applications.

show abstract

Section: Discussionmentioning

confidence: 99%

Nanocomposites obtained by incorporation of silanized silver nanowires to improve mechanical properties and prevent fungal adhesion

Menezes¹,

Sampaio

Silva³

et al. 2021

Nano Select

View full text Add to dashboard Cite

show abstract

“…While only a handful of works demonstrated microfluidic‐assisted identification of fungal species, there are numerous biosensing systems for fungal pathogens detection [ 127–130 ] which have not yet been coupled with microfluidics. For example, immunosensors such as antibody‐functionalized surface plasmon resonance (SPR) [ 129 ] and electrochemical impedance spectroscopy (EIS) [ 130 ] biosensors were employed for Candida cell detection. Thus, we envision that, in the future, the integration of these biosensors with available microfluidic devices [ 131 ] can be readily realized to advance the field.…”

Section: The Potential Of Microfluidics For Afstmentioning

confidence: 99%

“…This labeled proof-of-concept assay is only applicable for artificially derived GFP expressing yeasts; still, for wild-type strains, this approach could be potentially extended by employing fluorescently labeled antibodies or peptide nucleic acid-based fluorescence in situ hybridization. [125,126] While only a handful of works demonstrated microfluidicassisted identification of fungal species, there are numerous biosensing systems for fungal pathogens detection [127][128][129][130] which…”

Section: Step 2: Microfluidics For Fungal Pathogen Detection and Iden...mentioning

confidence: 99%

“…have not yet been coupled with microfluidics. For example, immunosensors such as antibody-functionalized surface plasmon resonance (SPR) [129] and electrochemical impedance spectroscopy (EIS) [130] biosensors were employed for Candida cell detection. Thus, we envision that, in the future, the integration of these biosensors with available microfluidic devices [131] can be readily realized to advance the field.…”

Section: Miniaturizationmentioning

confidence: 99%

See 1 more Smart Citation

Paving the Way to Overcome Antifungal Drug Resistance: Current Practices and Novel Developments for Rapid and Reliable Antifungal Susceptibility Testing

et al. 2021

View full text Add to dashboard Cite

treating IFI, rising resistance to these drugs is a cause of major concern. [1,3] For example, the globally emerging multidrug-resistant species Candida auris is now recognized by the Centers for Disease Control and Prevention (CDC) as an urgent threat. [4] Thus, its proper treatment is therefore crucial for both individual therapeutic outcomes and preventing its spread. [5] The increasing and undirected use of antifungals in medicine and agriculture is associated with the rising numbers of acquired resistance in fungi. [3] The readers are referred to the following excellent reviews on fungal pathogens [6,7] and antifungal resistance [3] for further reading. The latter, in combination with the challenges associated with developing novel antifungals, emphasizes the need for rapid disease detection and adequate antifungal therapy protocols. [3,8] Implementing such measures is part of a proper antimicrobial stewardship aimed at reducing the excessive usage of antimicrobial therapies in clinical settings by encouraging physicians to prescribe appropriate antimicrobials only when truly required. [9] Antimicrobial susceptibility testing (AST), specifically antifungal susceptibility testing (AFST), is employed to reveal the susceptibility or resistance of fungal pathogens to clinically relevant antifungals. [10,11] In these tests, pathogenic fungi are exposed to various concentrations of a panel of antifungals to determine the minimum inhibitory concentration (MIC) values, which are typically defined as the lowest drug concentration inhibiting the pathogen's growth. [12] MIC values allow physicians to predict the success of antifungal treatments. [13] As such, rapid AFST is an essential tool to improve antifungal therapy by choosing the correct and most effective antifungal drug in a timely manner. Early therapy initiation is also crucial for enhancing the therapeutic outcome of patients with invasive fungal infections, such as life-threatening bloodstream infections. [14] Clinical AFST is conducted according to standardized methods and protocols, published by the European Committee on Antimicrobial Susceptibility Testing (EUCAST) or the Clinical and Laboratory Standards Institute (CLSI). [12,15] Yet, as these gold-standard methods are laborious and typically require at least 24 h (and in many cases, several days) for completion, [12,16] significant research efforts are now being directed toward the development of more expedited phenotypic and molecular AFST techniques. [17,18] The past year has established the link between the COVID-19 pandemic and the global spread of severe fungal infections; thus, underscoring the critical need for rapid and realizable fungal disease diagnostics. While in recent years, health authorities, such as the Centers for Disease Control and Prevention, have reported the alarming emergence and spread of drug-resistant pathogenic fungi and warned against the devastating consequences, progress in the diagnosis and treatment of fungal infections is limited. Early diagnosis and patient-tailored t...

show abstract

“…Detection of Candida albicans, an opportunistic pathogenic yeast present in oral cavities, was detected using prism-based SPR imaging (83). The gold surface was functionalized with primary antibodies against C. albicans via amino coupling.…”

Section: Detection Of Bacteriamentioning

confidence: 99%

Advances in Surface Plasmon Resonance Imaging and Microscopy and Their Biological Applications

Bocková

Slabý

Špringer

et al. 2019

Annual Rev. Anal. Chem.

View full text Add to dashboard Cite

Surface plasmon resonance microscopy and imaging are optical methods that enable observation and quantification of interactions of nano- and microscale objects near a metal surface in a temporally and spatially resolved manner. This review describes the principles of surface plasmon resonance microscopy and imaging and discusses recent advances in these methods, in particular, in optical platforms and functional coatings. In addition, the biological applications of these methods are reviewed. These include the detection of a broad variety of analytes (nucleic acids, proteins, bacteria), the investigation of biological systems (bacteria and cells), and biomolecular interactions (drug–receptor, protein–protein, protein–DNA, protein–cell).

show abstract

Application of surface plasmon resonance biosensor for the detection of Candida albicans

Cited by 12 publications

References 30 publications

Nanocomposites obtained by incorporation of silanized silver nanowires to improve mechanical properties and prevent fungal adhesion

Nanocomposites obtained by incorporation of silanized silver nanowires to improve mechanical properties and prevent fungal adhesion

Paving the Way to Overcome Antifungal Drug Resistance: Current Practices and Novel Developments for Rapid and Reliable Antifungal Susceptibility Testing

Advances in Surface Plasmon Resonance Imaging and Microscopy and Their Biological Applications

Contact Info

Product

Resources

About