Application of surrogate broodstock technology in aquaculture

Yoshizaki, Goro; Yazawa, Ryosuke

doi:10.1007/s12562-019-01299-y

Cited by 87 publications

(37 citation statements)

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“…The cryopreservation of less differentiated cells such as spermatogonia, oogonia and primordial germ cells presents an alternative biobanking method for species in which it may be difficult to acquire mature gametes such as spermatozoa. Successful cryopreservation of rainbowfish gonadal tissue provides a readily available method for the preservation of a reproductive and genetic resource for rainbowfish and other Australian fish species 26 . The viability of spermatogonia was highest following cryopreservation with 1.3 M DMSO and 0.1 M trehalose in 1.5% BSA and a mixed salt solution.…”

Section: Discussionmentioning

confidence: 99%

Cryopreservation of testicular tissue from Murray River Rainbowfish, Melanotaenia fluviatilis

Rivers

Daly

Jones³

et al. 2020

Sci Rep

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Globally, fish populations are in decline from overfishing, habitat destruction and poor water quality. Recent mass fish deaths in Australia’s Murray–Darling Basin highlight the need for improved conservation methods for endangered fish species. Cryopreservation of testicular tissue allows storage of early sperm precursor cells for use in generating new individuals via surrogacy. We describe successful isolation and cryopreservation of spermatogonia in an Australian rainbowfish. Testis histology showed rainbowfish spermatogonia are large (> 10 μm) and stain positive for Vasa, an early germ line-specific protein. Using size-based flow cytometry, testis cell suspensions were sorted through “A” (> 9 μm) and “B” gates (2–5 μm); the A gate produced significantly more Vasa-positive cells (45.0% ± 15.2%) than the “B” gate (0.0% ± 0.0%) and an unsorted control (22.9% ± 9.5%, p < 0.0001). The most successful cryoprotectant for “large cell” (> 9 μm) viability (72.6% ± 10.5%) comprised 1.3 M DMSO, 0.1 M trehalose and 1.5% BSA; cell viability was similar to fresh controls (78.8% ± 10.5%) and significantly better than other cryoprotectants (p < 0.0006). We have developed a protocol to cryopreserve rainbowfish testicular tissue and recover an enriched population of viable spermatogonia. This is the first step in developing a biobank of reproductive tissues for this family, and other Australian fish species, in the Australian Frozen Zoo.

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Section: Discussionmentioning

confidence: 99%

Cryopreservation of testicular tissue from Murray River Rainbowfish, Melanotaenia fluviatilis

Rivers

Daly

Jones³

et al. 2020

Sci Rep

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“…These GSCs can be isolated and transplanted into the body of surrogate host by various methods. Host's genital ridge is in turn colonized by transplanted GSCs with subsequent proliferation and production of donor-derived gametes [9][10][11]. Of the utmost importance, GSCs have a high level of sexual plasticity.…”

Section: Introductionmentioning

confidence: 99%

Production of common carp donor-derived offspring from goldfish surrogate broodstock

Franěk

Kašpar

Gela

et al. 2020

Preprint

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Background: Common carp is the fourth most-produced species in worldwide aquaculture. Significant efforts are invested in breeding and preservation of genetic integrity of this important species. However, maintaining carp gene bank in situ can be considered as demanding due to its big body size. Recent progress in reproductive biotechnologies in fish allows improving some unfavourable characteristics of a target species using surrogate reproduction. Germ stem cells (gamete precursors) from one species are transplanted into different surrogate species with small body size. After maturation, surrogates are producing donor-derived progeny. Efficient protocols for cryopreservation of carp male and female germ stem cells have been developed lately. Thus, the next logical goal was to assess the potential of goldfish surrogate to produce donor-derived gametes of common carp after intraperitoneal transplantation of testicular cells. Results: High transplantation success was achieved when 44% of the surviving goldfish produced pure donor-derived gametes of common carp. More importantly, both viable eggs and sperm giving rise to pure common carp progeny were produced, witnessing sustainability of the presented method. Donor-derived identity of the offspring was confirmed by genotyping and typical phenotype corresponding to the donor species. Reproductive performance of chimeras was similar to goldfish controls. Assessment of gamete characteristics showed that the size of donor-derived eggs is between control carp and goldfish eggs. Interestingly, flagellum length in donor-derived spermatozoa was comparable to common carp flagellum and significantly shorter than goldfish flagellum. Conclusions: In this study, we succeeded in the production of pure common carp progeny from surrogate goldfish recipients transplanted intraperitoneally by testicular germ cells. Here we reported production of viable eggs between most distant species up to date. Good reproductive performance of goldfish germline chimeras gives a promising prospect for further analysis about the long-term reproductive performance of surrogates, recovery of cryopreserved germ cells or production of monosex stocks. Presented technology is ready to ease needs for carp breeds preservation and their recovery using many times smaller goldfish surrogates.

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“…We previously succeeded in establishing a germ cell transplantation system in various teleosts (Farlora et al, ; Hamasaki et al, ; Li, Fujii, Naito, & Yoshizaki, ; Morita et al, ; Morita et al, ; Octavera & Yoshizaki, ; Okutsu, Shikina, Kanno, Takeuchi, & Yoshizaki, ; Okutsu, Suzuki, Takeuchi, Takeuchi, & Yoshizaki, ; Seki et al, ; Takeuchi, Yoshizaki, & Takeuchi, ; Yoshikawa et al, ; Yoshizaki & Yazawa, ). In this transplantation system, A spermatogonia (ASGs) or oogonia isolated from donor gonads were transplanted into the peritoneal cavity of newly hatched larvae that did not yet have a mature immune system.…”

Section: Introductionmentioning

confidence: 99%

“…The development and maturation of donor ASGs and oogonia in the recipient's gonads are most likely regulated by somatic environments created by the recipient (Okutsu et al, ; Yoshizaki et al, ). Therefore, if ASGs and oogonia of donor fish with a long generation time, such as Chinook salmon and sockeye salmon, are transplanted into rainbow trout, which mature in a short period, donor gametes are expected to be produced in 1–2 years (Yoshizaki & Yazawa, ). In addition, functional gametes can be produced from a recipient receiving ASGs and oogonia that were retrieved from either cryopreserved gonads or frozen whole fish (Kobayashi, Takeuchi, Takeuchi, & Yoshizaki, ; Lee et al, ; Lee, Iwasaki, & Yoshizaki, ; Lee, Seki, Katayama, & Yoshizaki, ; Yoshizaki & Lee, ).…”

Section: Introductionmentioning

confidence: 99%

“…transplanted into rainbow trout, which mature in a short period, donor gametes are expected to be produced in 1-2 years (Yoshizaki & Yazawa, 2019). In addition, functional gametes can be produced from a recipient receiving ASGs and oogonia that were retrieved from either cryopreserved gonads or frozen whole fish (Kobayashi, Takeuchi, Takeuchi, & Yoshizaki, 2007;Lee et al, 2013;Lee, Iwasaki, & Yoshizaki, 2016;Lee, Seki, Katayama, & Yoshizaki, 2015;Yoshizaki & Lee, 2018).…”

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confidence: 99%

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Enrichment of transplantable germ cells in salmonids using a novel monoclonal antibody by magnetic‐activated cell sorting

Ichida

Hayashi

Miwa

et al. 2019

Molecular Reproduction Devel

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In the fish germ cell transplantation system, only type A spermatogonia (ASGs) and oogonia are known to be incorporated into the recipient genital ridges, where they undergo gametogenesis. Therefore, high colonization efficiency can be achieved by enriching undifferentiated germ cells out of whole testicular cells. In this study, we used magnetic‐activated cell sorting (MACS) for enriching undifferentiated germ cells of rainbow trout using a monoclonal antibody that recognizes a specific antigen located on the germ cell membrane. We screened the antibodies to be used for MACS by performing immunohistochemistry on rainbow trout gonads. Two antibodies, nos. 172 and 189, showed strong signals for ASGs and oogonia. Next, we performed MACS with antibody no. 172 using gonadal cells isolated from vasa‐gfp rainbow trout showing GFP in undifferentiated germ cells. We found that GFP‐positive cells are highly enriched in antibody no. 172‐positive fractions. Finally, to examine the transplantability of MACS‐enriched cells, we intraperitoneally transplanted sorted or unsorted cells into recipient larvae. We observed that transplantability of sorted cells, particularly ovarian cells, were significantly higher than that of unsorted cells. Therefore, MACS with antibody no. 172 could enrich ASGs and oogonia and become a powerful tool to improve transplantation efficiency in salmonids.

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Application of surrogate broodstock technology in aquaculture

Cited by 87 publications

References 50 publications

Cryopreservation of testicular tissue from Murray River Rainbowfish, Melanotaenia fluviatilis

Cryopreservation of testicular tissue from Murray River Rainbowfish, Melanotaenia fluviatilis

Production of common carp donor-derived offspring from goldfish surrogate broodstock

Enrichment of transplantable germ cells in salmonids using a novel monoclonal antibody by magnetic‐activated cell sorting

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