The development of antimicrobial materials and its use have shown an effective way to reduce the risks posed to human life by fungi, bacteria and other pathogenic microorganisms. This work presents preliminary results about the development of powder biocidal vitreous, applying the ionic exchange process between the sodium ions, present in the glass matrix, and ionic oligodynamic species, present in the ionic exchange medium. The magnitude of the oligodynamic effect was modified with the use of different metal ions, providing significant difference in antimicrobial performance. Three samples were prepared incorporating different ionic metal in its glass matrix during the ionic exchange process, Ag+, Zn2+ and Cu2+. These precursors of cations were used: AgNO3 for the silver ions, ZnSO4 for the zinc ions and Cu (NO3)2 for the cooper ions. Samples were submitted to microbiological analysis – Agar Diffusion Test for Escherichiacoli and Staphylococcusaureus bacterial species, and fungus species Candidaalbicans – and atomic absorption techniques. Results showed higher microbiological magnitude to the silver-glass, followed by the cooper-glass and zinc-glass.